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Anti icos

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Anti-ICOS is a laboratory reagent that specifically binds to the ICOS (Inducible T-Cell COStimulator) molecule. ICOS is a cell surface protein expressed on activated T cells and plays a role in T cell activation and function. Anti-ICOS can be used in various research applications to study ICOS-mediated signaling and T cell biology.

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Lab products found in correlation

Anti cd3 anti cd28 beads, by Thermo Fisher Scientific (1 mentions) Anti ctla4, by BioXCell (1 mentions) Interleukin 7 (il 7), by BioXCell (1 mentions) Ctla4 ig, by Bristol-Myers Squibb (1 mentions) Mouse igg2a, by BioXCell (1 mentions) Ifn γ, by BioXCell (1 mentions) Cd40l, by BioXCell (1 mentions) Anti cd86, by BioXCell (1 mentions) Anti pd 1, by BioXCell (1 mentions) Mouse igg1, by BioXCell (1 mentions) Rat igg2a, by BioXCell (1 mentions) Ctla4 ig, by BioXCell (1 mentions)

Spelling variants of this product

Anti-ICOS-L (4 citations) Anti-mouse ICOS (7E.17G9) (2 citations)

3 protocols using anti icos

1

Regulatory T Cell-Mediated Suppression of CD4+ T Cell Proliferation

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Tregs and CD4+ Tconv cells were purified from the lung tumor and blood specimens (3 × 103/well). CD4+ Tconv cells were labeled with CTV (life technologies, France, working concentration: 10 µM), and then cultured with anti-CD3/anti-CD28 beads (1 bead/10 cells, Invitrogen) and without any exogenous cytokine in 96 well round bottom plates in complete RPMI 1640 with 10% AB+ heat-inactivated human serum for 72 h at 37 °C. Autologous Tregs were co-cultured with CD4+ Tconv cells at different ratios (0:1, 1:1 and 2:1). CD4+ Tconv proliferation was measured on Day 3 by analyzing CTV dilution by flow cytometry. For some experiments, cells were cultured with neutralizing antibodies against anti-GITR (clone DT5D3, Miltenyi Biotec), anti-CTLA-4 (clone BN13, BioXCell), anti-ICOS (clone 314.8 kindly provided by Dr. D. Olive), anti-PD-1 (clone J116, BioXCell), anti-TIGIT (clone MBSA43, Affymetrix), and anti-Tim-3 (clone F38-2E2, Affymetrix), (working concentration at 10 μg/mL).
Devi-Marulkar P., Fastenackels S., Karapentiantz P., Goc J., Germain C., Kaplon H., Knockaert S., Olive D., Panouillot M., Validire P., Damotte D., Alifano M., Murris J., Katsahian S., Lawand M, & Dieu-Nosjean M.C. (2022). Regulatory T cells infiltrate the tumor-induced tertiary lymphoïd structures and are associated with poor clinical outcome in NSCLC. Communications Biology, 5, 1416.
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2

Antibody Modulation of Immune Checkpoints

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C57BL/6 mice were injected with following mAbs as indicated in figure legends: 250 μg/dose mouse IgG1 (Clone: MOPC-21; BioXcell), mouse IgG2a (Clone: C1.18.4), Rat-IgG2a (Clone: 2A3; BioXcell), of human CD28 domain specific antibody (100 μg/dose; Bristol-Myers Squibb), 250 μg/dose of anti-CD80 (Clone: 1G10; BioXcell), anti-CD86 (Clone: GL-1; BioXcell) CTLA4-Ig (Abatacept, Bristol-Myers Squibb), Anti-ICOS (Clone: 7E.17G9; BioXcell), Anti-CTLA4 (Clone UC10-4F10-11; Bio X Cell); Anti-PD1(Clone: 29F.1A12; BioXcell), TIM-3(Clone: RMT3.23; BioXcell), TIGIT (Clone:1G9; BioXcell), BTLA (Clone:6A6, BioXcell), VISTA(Clone: 13F3; BioXcell), CD70(Clone: FR70; BioXcell), CD48(Clone: HM48.1; BioXcell), TNF-α (Clone: XT3.11; BioXcell), IFNγ (Clone: XMG1.2; BioXcell), IFNAR (Clone:MAR1–5A3; BioXcell), IFNγR (Clone:GR20), IL-7 (Clone:M25; BioXcell), IL-7Rα (Clone:A7R34; BioXcell), CD40 (50 μg/dose; Clone:FGK4.5; BioXcell, CD40L (MR-1; BioXcell) Clone:, GITR (Clone:DTA-1; BioXcell), OX40 (Clone:OX-86; BioXcell), 4-1BB (Clone:LOB12.3; BioXcell), and Anti-MHC-I (500 μg/dose; Clone: M1/42; BioXcell).
Panda A.K., Kim Y, & Shevach E.M. (2022). Control of Memory Phenotype T Lymphocyte Homeostasis: Role of Costimulation. Journal of immunology (Baltimore, Md. : 1950), 208(4), 851-860.
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3

Allogeneic Skin Transplant with Immunomodulation

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Full-thickness tail and ear skins were transplanted onto the dorsal thorax of recipient mice and secured with adhesive bandages as previously described (Trambley et al., 1999). In some experiments, mice were treated with 250 μg/dose of CTLA-4 Ig (BioXCell) and anti-ICOS (BioXCell) via i.p. injection on day 0, 2, 4, and 6.
Cohen G.S., Kallarakal M.A., Jayaraman S., Ibukun F.I., Tong K.P., Orzolek L.D., Benjamin Larman H, & Krummey S.M. (2023). Transplantation elicits a clonally diverse CD8+ T cell response that is comprised of potent CD43+ effectors. Cell reports, 42(8), 112993.
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