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Sevencompact ph ion s220

Manufactured by Mettler Toledo
Sourced in Switzerland, United States

The SevenCompact pH/Ion S220 is a compact and versatile pH/ion meter from Mettler Toledo. It features a large, easy-to-read display and intuitive user interface. The device is capable of measuring pH, mV, and ion concentration with high accuracy and precision.

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14 protocols using sevencompact ph ion s220

1

Reconstitution and Characterization of SPIONs

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To reconstitute the SPIONs from the electrospun products and reference dry products (i.e., oven-dried and lyophilized products), 10 mL of purified water was added to the sample (~10 mg) in a 20 mL glass vial and mixed well by 3 min vortex mixing. The dispersion was visually inspected to confirm the reconstitution. Next, the hydrodynamic size and zeta potential of SPIONs were evaluated, as described in Section 2.3. The short-term physical stability of the SPION dispersions after reconstitution was evaluated by measuring the hydrodynamic size and zeta potential of SPIONs every 30 min in the first hour after the reconstitution. The pH value of the reconstituted SPION dispersions was measured with the pH meter SevenCompactTM pH/Ion S220 equipped with InLab® Expert Pro-ISM electrode (Mettler-Toledo; Greifensee, Switzerland). All reconstitution experiments were performed in triplicates, and the results are expressed as an average with the corresponding standard deviation.
Additionally, a drop of the reconstituted SPION dispersion was placed on a carbon-coated copper TEM grid, air-dried at room temperature, and evaluated by transmission electron microscopy (TEM; Jem 2100; Jeol, Akishima, Japan). TEM imaging was performed using an accelerating voltage of 200 kV.
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2

Broiler Growth and Gizzard pH

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Body weight and feed intake were recorded weekly on a pen basis until the end of the 5th week. Body weight gain and feed consumption were determined weekly, and feed conversion ratio was calculated using the weekly weight gains and feed consumption values.
At 35 D of age, pH of the gizzards from12 birds per treatment group were measured using a digital pH meter (Mettler Toledo, SevenCompactTM pH/Ion S220, Greifensee, Switzerland).
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3

Reconstitution and Characterization of Electrospun MNPs

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After the drying of MNP dispersions via electrospinning, the reconstitution of MNPs was performed as follows. The dry product (~10 mg) was weighed into 20 mL glass vial and 10 mL of 0.9 % (w/v) NaCl solution was added. The sample in the glass vial was mixed well by a vortex mixer for 3 min to achieve the reconstitution, which was confirmed by a visual inspection of the sample. After the reconstitution, the samples were diluted to the final concentration of 0.1 mg MNPs/mL with 0.9 % (w/v) NaCl solution, and average hydrodynamic size and zeta potential were determined (Zetasizer Ultra, Malvern Panalytical Ltd.; Worcestershire, UK). The hydrodynamic size, size distribution, and zeta potential of MNPs were expressed relative to values measured fot MNPs in the initial dispersions diluted with 0.9 % (w/v) NaCl solution.
To assess the short-term physical stability of the MNP dispersion after reconstitution, the measurements of hydrodynamic size and zeta potential were performed every 15 min in the first hour after the reconstitution. The pH value of the dispersions right after the reconstitution of MNPs was measured with pH meter SevenCompact TM pH/Ion S220 equipped with InLab® Expert Pro-ISM electrode (Mettler-Toledo; Greifensee, Switzerland). All experiments were performed in triplicates.
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4

Optimizing EV Zeta Potential via PBS

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To study the effect of PBS concentration on the ZP
of EVs, 20 μL of the JAr EVs was suspended in a final volume
of 200 μL of PBS diluted in MQ water in a ratio of 1:1000 to
obtain an initial concentration in the range of 109 particles/mL.
ZetaView NTA was used to measure the hydrodynamic size profile and
concentration of JAr EVs suspended in 1:10 and 1:100 diluted PBS/MQ.
Using such PBS dilutions (1, 0.1, and 0.01 mM), samples with diverse
EV concentrations, ranging from 106 to 108 EVs/mL,
were prepared and incubated at 4 °C for 3 h on a BIOSAN Multi
Bio RS-24 programmable rotator (BioSan, Riga, Latvia) set at 30 rpm.
After the incubation, the pH of the suspension medium was adjusted
to 6.9 with a pH meter (SevenCompactTM pH/Ion S220, Mettler-Toledo
AG, Schwerzenbach, Switzerland) by addition of 0.1 M HCl/NaOH. The
ZP of JAr EV samples was measured using ZetaView software. The experiments
were performed on 3 days, and on each day, triplicates of ZP measurements
were taken for individual samples with different concentrations of
JAr EVs.
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5

Physicochemical Characterization of Ecuadorian Fruits

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Weight, length, and diameter were determined according to the methods described by the Ecuadorian Technical Standards 2427 [12 ]. The pH of the fruits was analyzed using a pH meter, SevenCompact pH/Ion S220 (Mettler Toledo, Greifensee, Switzerland), following the method described by the Association of Official Analytical Chemists (AOAC method 981.12) [13 ]. The method used to determine the moisture of the fruits included drying to constant weight in a vacuum oven at 70 °C and 100 mmHg pressure until a high level of water evaporation (max. 6 h) (AOAC method 950.27) was reached [13 ]. The results were expressed in percentage (g H2O 100 g−1 of sample). Soluble solids were assayed using a Portable Brix Refractometer VBR90S (Boeco, Germany) (AOAC method 931.12) [13 ]. The results were expressed as °Brix. The titratable acidity was determined by the method suggested by AOAC (method 942.15) [13 ], performing titration with NaOH 0.1 N. The results were expressed in % of citric acid. Every assay was conducted in triplicate.
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6

pH Measurement of TPN Admixture

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For the evaluation of pH, a Mettler Toledo Seven Compact pH/ion S220® pH-meter was used. The pH was measured by dipping the electrode directly into the TPN admixture, at room temperature. The pH of each sample was measured in triplicate.
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7

Biomass Composition and Biogas Analysis

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The neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) were determined using an ANKOM 2000 I, fiber Analyzer (ANKOM Technology Corporation, NY 14502, USA).
The dry matter content (TS) was analyzed with a moisture analyzer Ohaus MB 45. The volatile solids (VS) were analyzed as loss on ignition at 550 °C. The pH of the samples before and after pretreatment, hydrolysis, and fermentation was measured using a pH meter, model SevenCompact pH/Ion S220 from Mettler-Toledo AG (Schwerzenbach, Switzerland).
Glucose, glycerol, acetic acid, and ethanol were quantified by HPLC (LC-2030C Plus, Shimadzu, Kyoto, Japan) equipped with a refractive index detector (RID-20A, Shimadzu, Kyoto, Japan) using a Rezex ROA Organic Acid column (Phenomenex, Torrance, CA, USA) column at 45 °C, and isocratic elution at 0.6 mL/min of 5 mmol/L H2SO4.
The quantification of methane in the produced biogas was done by gas chromatography (CP-4900 Micro-GC, from Varian Inc., Palo Alto, USA).
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8

Physicochemical Stability of PN Admixtures

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Physical stability studies of PN admixtures included a visual examination and determination of pH, osmolarity, MDD of lipid emulsion, and zeta potential. All measurements were performed in triplicate for PN admixtures and MPM–PN admixture samples and expressed as mean ± standard deviation.
According to the European Pharmacopoeia [23 ], all samples were visually assessed against a black-and-white contrast background by two observers for lack of visible particles and color changing. The evaluation of pH was performed using pH-meter Seven Compact pH/ion S220 (Mettler Toledo, Greifensee, Switzerland). The MDD of lipid emulsion and zeta potential of PN admixtures were measured using Zetasizer Nano ZS (Malvern Instruments, Malvern, UK) at 25 °C according to US Pharmacopoeia [20 ]. The osmolarity was measured at room temperature using an 800CL TridentMed® osmometer (Trident Med s.c., Warsaw, Poland).
Samples for particle size and zeta potential measurement were prepared following the same procedure, where 1 mL of MPM–PN admixture samples was diluted 10 times with water for injection. Then, 1 mL of diluted sample was transferred to a measurement cell for particle size and zeta potential determination, using DLS and laser Doppler electrophoresis (LDE) methods, respectively.
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9

Analysis of Organic Compounds in Food Samples

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Ten g of sample was homogenized with 90 mL of distilled water and the pH was measured using a digital glass pH meter (Mettler Toledo, SevenCompact™ pH Ion S220, Switzerland). For determination of TTA, sample homogenate was titrated with 0.1 N NaOH to a final pH of 8.5. TTA was expressed as the amount (mL) of 0.1 M NaOH needed to achieve the pH of 8.5.
Maltose, sucrose, glucose, fructose, ethanol, lactic and acetic acids were determined in the extracts through an HPLC system consisting of a refractive index detector (RID-10A) for sugar and ethanol analyses and a UV/Vis detector (SPD-20A) monitored at 210 nm for organic acid analyses as reported previously (Boyaci-Gunduz & Erten, 2020 (link)). Extraction was done according to the method of Paramithiotis et al. (2006) (link). An Aminex HPX-87H column (300 × 7.8 mm, Bio-Rad, Hercules, CA, USA) was used for chromatographic separation. HPLC analyses were carried out in duplicate.
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10

HPLC-Amperometric Detection System

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The HPLC was coupled, in
sequence, to a flow cell obtained from Metrohm UK, Runcorn, U.K. (impinging
jet flow cell; product code: DRP-FLWCL-TEF-71306; 3.3 × 6.0 ×
3.3 cm3, flow chamber volume = 8 μL), housing the
SPE to give the HPLC-AD system. The connection between the DAD and
the flow cell was achieved via PTFE tubing (230 × 1.6 mm2, i.d. 0.3 mm, internal volume: 16.25 μL). The SPEs
utilized in this part of the study were fabricated in-house, as previously
described,42 (link),51 (link) and consisted of a 3.1 mm diameter
graphite working electrode, a graphite counter electrode, and a Ag/AgCl
reference electrode. Amperometric measurements were carried out using
an EmStat 3 potentiostat/galvanostat (PalmSens BV, The Netherlands)
and controlled by PSTrace (version 4.4) software (PalmSens, The Netherlands).
All of the amperometric measurements were carried out using the following
parameters: (i) potential (E, +1.0 V); (ii) equilibration
time (tequibriation, 10.0 s); (iii) data
interval (tinterval, 0.08 s); (iv) current
range (100 nA to 1 mA); and (iv) total run time (trun, 5000 s). A new SPE was used for each experiment performed.
Buffer pH measurements were made using a “SevenCompact pH/Ion
S220” (Mettler-Toledo AG, Switzerland) pH meter.
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