Western blot analysis was performed as previously described protocol (Jeon et al., 2021 (link)). The primary antibodies used were anti-HMGB-1 (Abcam, Cambridge, MA, United States), anti-β-actin (Sigma-Aldrich), anti-KSHV ORF 50 (Bioss, Woburn, MA, United States), anti-HHV ORF 45 (Thermo Fisher Scientific), and anti-KSHV K8.1 antibodies (Santa Cruz, Santa Cruz, CA, United States). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Bethyl; Montgomery, TX, United States) and HRP-conjugated goat anti-rabbit IgG (Bethyl) were used as secondary antibodies. The antibody-reacted membranes were visualized using Amersham ImageQuant 800.
Anti kshv orf 50
Manufactured by Bioss Antibodies
Sourced in United States
Anti-KSHV ORF 50 is a laboratory product designed for research purposes. It is an antibody that specifically targets the Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 50 protein. This product can be used in various research applications to study the biology and characteristics of KSHV.
Automatically generated - may contain errors
Lab products found in correlation
Anti hmgb1, by Abcam (2 mentions)
Anti β actin, by Merck Group (1 mentions)
Imagequant 800, by Cytiva (1 mentions)
Hrp conjugated goat anti rabbit igg, by Fortis Life Sciences (1 mentions)
Hrp conjugated goat anti mouse igg, by Fortis Life Sciences (1 mentions)
Amersham imagequant, by Cytiva (1 mentions)
Alexa fluor 568 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Eclipse e400 microscope, by Nikon (1 mentions)
2 protocols using anti kshv orf 50
1
Western Blot Analysis of HMGB-1, KSHV, and HHV
2
Immunofluorescence Assay for HMGB1, KSHV Proteins
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Immunofluorescence assay (IFA) was performed as previously described (Lee M. S. et al., 2014 (link); Kang et al., 2021a (link)). The primary antibodies used were anti-HMGB-1 (Abcam), anti-KSHV ORF50 (Bioss), anti-KSHV K8.1 (Santa Cruz), and anti-LNA (Abcam) antibodies. The secondary antibodies used were Alexa Fluor 568-conjugated anti-mouse IgG (Thermo Fisher Scientific) and Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific) antibodies. A concentration of 500 ng/mL of 49,6-diamidino-2-phenylindole (DAPI) was used to stain nucleic acids. The stained samples were observed under a Nikon Eclipse E400 microscope (Nikon, Tokyo, Japan) under the same conditions.
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