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Rabbit anti human fibronectin

Manufactured by Merck Group
Sourced in Germany, United States

Rabbit anti-human fibronectin is a laboratory reagent used to detect and quantify the presence of human fibronectin in biological samples. Fibronectin is a large extracellular glycoprotein involved in cell adhesion and migration processes. This product uses a rabbit-derived antibody that specifically binds to human fibronectin, allowing for its identification and measurement in various experimental and diagnostic applications.

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4 protocols using rabbit anti human fibronectin

1

Antibodies for Western Blot and Immunofluorescence Analysis

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Mouse monoclonal anti-human Hsp90α/β (cat no.: sc-13119) and goat polyclonal anti-human Hsp90α/β (cat no: sc-1055) primary antibodies were from Santa Cruz Biotechnology (USA). Mouse anti-human fibronectin (cat no.: F0916), rabbit anti-human fibronectin (cat no.: F3648), rabbit anti-human actin (cat no.: A2103) primary antibodies and goat anti-mouse IgG-HRP (cat no.: A2304) secondary antibody were from Sigma Aldrich (Germany). Rabbit monoclonal anti-human LRP1 (ab92544), mouse monoclonal [TV.1] anti-human fibronectin (ab194395), rabbit polyclonal anti-human histone H3 (ab1791) primary antibodies and donkey anti-rabbit IgG-HRP (ab16284) secondary antibody were from Abcam (UK). Donkey anti-mouse Dylight® 488 (ab96875), donkey anti-rabbit Dylight® 555 (ab96892) and donkey anti-goat Dylight® 650 (ab96934) secondary antibodies were also from Abcam (UK). Mouse monoclonal anti-human LRP1 blocking antibody (GTX79843) was from GeneTex (USA). Alexa Fluor-488 conjugated donkey anti-mouse IgG (cat no.: A21202), Alexa Fluor-546 conjugated donkey anti-rabbit IgG (cat no.: A10040), Alexa Fluor-660 conjugated donkey anti-goat IgG (cat no.: A21082) were from Invitrogen (UK).
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2

Antibody Characterization for Cellular Signaling

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Rabbit anti-human Smad2/3 and phospho-Smad3 antibodies were obtained from Sigma-Aldrich. Rabbit anti-human/mouse/rat LC3β II was obtained from Cell Signaling (Danvers, USA).
Collagen 1α2 (goat anti-human), GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (mouse anti-human), and rabbit anti-human fibronectin were obtained from Sigma-Aldrich. Secondary antibodies, horseradish peroxidase and fluorochrome conjugated, were obtained from Bioworld Technology Inc., Louis Park, MN, USA.
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3

Immunohistochemical Markers of Fibrosis

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The primary antibodies that were used for immunohistochemistry and immunoblotting included rabbit anti-p-c-fms/CSF-1R(Tyr723) and nitrotyrosine (marker of oxidative stress), goat anti-CSF-1 and human connective tissue growth factor (CTGF) from Santa Cruz Biotechnology; rat anti-F4/80 (a marker of macrophage/dendritic cell) and Ly6G (Gr-1, a marker of neutrophils) from AbD Serotec; rabbit anti-HMGB1 from Cell Signaling Technology; mouse anti-Kim-1 (a marker of renal tubular injury, MAB1817) and mannose receptor (MR, CD206) from R&D Systems, mouse anti-α-smooth muscle actin (α-SMA, a marker of myofibroblasts) and rabbit anti-human fibronectin from Sigma-Aldrich.
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4

Immunohistochemical Markers of Fibrosis

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The primary antibodies that were used for immunohistochemistry and immunoblotting included rabbit anti-p-c-fms/CSF-1R(Tyr723) and nitrotyrosine (marker of oxidative stress), goat anti-CSF-1 and human connective tissue growth factor (CTGF) from Santa Cruz Biotechnology; rat anti-F4/80 (a marker of macrophage/dendritic cell) and Ly6G (Gr-1, a marker of neutrophils) from AbD Serotec; rabbit anti-HMGB1 from Cell Signaling Technology; mouse anti-Kim-1 (a marker of renal tubular injury, MAB1817) and mannose receptor (MR, CD206) from R&D Systems, mouse anti-α-smooth muscle actin (α-SMA, a marker of myofibroblasts) and rabbit anti-human fibronectin from Sigma-Aldrich.
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