6-O-capro-β-CD (MW = 1813 g/mol) and poly-β-CD-C6 (MW = 3178 g/mol) seen in Figure 9 were synthetized, purified, and characterized in the Institute for Chemical Research (CSIC-University of Sevilla, Spain) as previously reported [9 (link),25 (link),50 (link)].
(S)-(+)-Camptothecin (95% HPLC powder, MW: 348.35 g/mol) was purchased from Sigma-Aldrich, USA. Chitosan (Protasan UP G-113; MW < 200 kDa) was purchased from Novamatrix, Norway. Dialysis cellulose tubing membrane for in vitro release studies (average flat width 25 mm, MWCO: 14,000 Da) was obtained from Sigma-Aldrich, USA. Cell culture studies were performed on CT26 mouse (ATCC® CRL-2638™) and HT29 human colon carcinoma cell line (ATCC® HTB-38™) both purchased from American Type Culture Collection (ATCC, USA). Dulbecco’s modified Eagle medium (DMEM, D5796, Sigma-Aldrich, USA), supplemented with 10% (v/v) fetal bovine serum (FBS, F7524, Sigma-Aldrich, USA) and 1% penicillin/streptomycin (P4333, Sigma-Aldrich, USA) was used for 2D and 3D cell culture studies. Ultrapure water was obtained from a Millipore Simplicity 185 Ultrapure Water System (Millipore, France). All other chemicals were purchased from Sigma-Aldrich and were of analytical purity. Release kinetic analyses were performed using an add-in program, DDSolver 1.0 [73 (link)].
(S)-(+)-Camptothecin (95% HPLC powder, MW: 348.35 g/mol) was purchased from Sigma-Aldrich, USA. Chitosan (Protasan UP G-113; MW < 200 kDa) was purchased from Novamatrix, Norway. Dialysis cellulose tubing membrane for in vitro release studies (average flat width 25 mm, MWCO: 14,000 Da) was obtained from Sigma-Aldrich, USA. Cell culture studies were performed on CT26 mouse (ATCC® CRL-2638™) and HT29 human colon carcinoma cell line (ATCC® HTB-38™) both purchased from American Type Culture Collection (ATCC, USA). Dulbecco’s modified Eagle medium (DMEM, D5796, Sigma-Aldrich, USA), supplemented with 10% (v/v) fetal bovine serum (FBS, F7524, Sigma-Aldrich, USA) and 1% penicillin/streptomycin (P4333, Sigma-Aldrich, USA) was used for 2D and 3D cell culture studies. Ultrapure water was obtained from a Millipore Simplicity 185 Ultrapure Water System (Millipore, France). All other chemicals were purchased from Sigma-Aldrich and were of analytical purity. Release kinetic analyses were performed using an add-in program, DDSolver 1.0 [73 (link)].