Human embryonic kidney (HEK) 293T cells were seeded in a 75 cm2 cell culture flask (4 × 106 cells per culture dish) with DMEM containing 10% FBS. The expression vector and lentivirus packaging mixture were transfected into HEK 293T cells with Hillymax (Dojindo, Kumamoto, Japan) and opti-MEM medium (Thermo Fisher Scientific, MA, USA). After transfection for 5 h, the primary mixture was replaced with DMEM medium with 1.0 g/L glucose and 5% FBS, and the supernatant was collected at 48 h after transfection. The collected supernatant containing viral vector was filtered by 0.2 μm-pore size syringe filter and stored at -80°C.
Hillymax
Manufactured by Dojindo Laboratories
Sourced in Japan
Hillymax is a laboratory equipment product manufactured by Dojindo Laboratories. It is designed to perform core functions related to laboratory operations, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using hillymax
1
Lentiviral Vector Production in HEK293T Cells
2
Lentivirus Production in HEK 293T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human embryonic kidney (HEK) 293T cells were seeded in a 75 cm2 cell
culture flask with DMEM containing 10% FBS without antibiotics. The expression
vector and lentivirus packaging vector mixture were transfected into HEK 293T
cells with Hillymax (Dojindo, Kumamoto, Japan) and DMEM (Thermo Fisher
Scientific, MA, USA). After transfection for 5 hours, the primary media with
vectors were replaced with antibiotic-free DMEM containing 1.0 g/L glucose, 5%
FBS. The supernatant containing lentivirus was collected at 48 hours after
transfection, filtered through a 0.4 μm-pore size syringe filter, and stored at
−80°C.
culture flask with DMEM containing 10% FBS without antibiotics. The expression
vector and lentivirus packaging vector mixture were transfected into HEK 293T
cells with Hillymax (Dojindo, Kumamoto, Japan) and DMEM (Thermo Fisher
Scientific, MA, USA). After transfection for 5 hours, the primary media with
vectors were replaced with antibiotic-free DMEM containing 1.0 g/L glucose, 5%
FBS. The supernatant containing lentivirus was collected at 48 hours after
transfection, filtered through a 0.4 μm-pore size syringe filter, and stored at
−80°C.
3
Lentiviral Vector Production in HEK293T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using Hillymax (Dojindo, Japan), human embryonic kidney (HEK) 293T cells were transfected with the cloned expression vector and lentivirus packaging vectors. Following 5 h of incubation, the vector-containing media were replaced with media containing 5% FBS, without antibiotics. The virus was acquired approximately 48 h later, passed through a 0.4 μm syringe filter, and maintained at -80°C until use.
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