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Alpha 300 raman spectrometer

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The Alpha 300 Raman spectrometer is a high-performance laboratory instrument designed for advanced materials analysis. It utilizes Raman spectroscopy, a powerful technique that provides detailed information about the chemical composition and molecular structure of samples. The Alpha 300 is capable of acquiring high-resolution Raman spectra, enabling users to obtain precise data about the materials under investigation.

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Lab products found in correlation

Tecnai t20 transmission electron microscope, by Thermo Fisher Scientific (1 mentions) D8 advance xrd, by Bruker (1 mentions) Jsm 6301f, by JEOL (1 mentions) Molybdenum iv oxide, by Thermo Fisher Scientific (1 mentions) Eclipse lv100nd microscope, by Nikon (1 mentions) Project plus software, by WITec (1 mentions)

8 protocols using alpha 300 raman spectrometer

1

Raman Analysis of TEM Grid Samples

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Raman spectra of the TEM grid samples
were obtained using a confocal WITec alpha 300 Raman spectrometer.
The spectra were recorded with a 532 nm laser excitation light and
an integration time of 2 s after each preparation step (i.e., air-annealed, nitridated, and TiOxNy–Ir samples) and finally after EC-STAT
analysis. The air-annealed form of the sample was extremely sensitive
to laser light excitation, demanding a low laser power of 0.6 mW and
also a diminished number of scans [from 100 (a) to 20 (b,c) scans
in Figure S1]. These spectra are background
corrected (due to fluorescence), while spectra of others are shown,
as measured in insets in Figure S2. For
measurements of other samples (nitridated, TiOxNy–Ir and after EC-STAT),
we used a protocol that can serve as a sample stability estimation.
Namely, we measured single Raman spectra sequentially at a certain
site using increasing laser powers of 0.6, 1.4, 3.4, 7.3, and 13.5
mW. Each sample on the TEM grid was examined at 3 sites. At higher
laser powers, the samples suffered degradation, but the extent of
degradation can be taken as a measure for the stability of the sample.
The results are shown in the Supporting Information in Figure S2.
Bele M., Podboršek G.K., Lončar A., Jovanovič P., Hrnjić A., Marinko Ž., Kovač J., Surca A.K., Kamšek A.R., Dražić G., Hodnik N, & Suhadolnik L. (2023). “Nano Lab” Advanced Characterization Platform for Studying Electrocatalytic Iridium Nanoparticles Dispersed on TiOxNy Supports Prepared on Ti Transmission Electron Microscopy Grids. ACS Applied Nano Materials, 6(12), 10421-10430.
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2

Multimodal Characterization of BN Nanoconjugates

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Attenuated total
reflectance Fourier-transform infrared (ATR–FTIR) spectra were
recorded on a Bruker’s Alpha spectrometer, utilizing a diamond
crystal as the refractive element. The spectra were acquired by averaging
256 scans at a resolution of 4 cm–1. Powder X-ray
diffraction (PXRD) was performed using a Bruker XRD D8 ADVANCE with
Cu Kα1 radiation. Data were collected with 2θ from 20
to 70°. Raman spectra were obtained using a WITec alpha300 Raman
spectrometer equipped with a 100× objective lens. A 532 nm wavelength
laser and a 600 g/mm grating were employed for excitation. Spectra
were acquired in the range of 500–3000 cm–1 with a 0.5 s integration time. The surface chemistry was analyzed
using an X-ray photoelectron spectroscopy (XPS) instrument (PHI VersaProbe
III). Monochromatized Al Kα X-ray (1486.6 eV) was employed to
collect the spectra. Spectra were obtained using CasaXPS software.
Dynamic light scattering (DLS) (Zetasizer Nano ZS, Japan) was used
to study the size distribution and zeta potential of BN nanoconjugates.
The shape and size of BN nanoconjugates was examined by scanning electron
microscopy (SEM, JEOL JSM 6301F) under an acceleration voltage of
5 kV. Transmission electron microscopy (TEM) was carried out using
a FEI Tecnai T20 transmission electron microscope under an acceleration
voltage of 200 kV.
Zhang J., Neupane N., Dahal P.R., Rahimi S., Cao Z., Pandit S, & Mijakovic I. (2023). Antibiotic-Loaded Boron Nitride Nanoconjugate with Strong Performance against Planktonic Bacteria and Biofilms. ACS Applied Bio Materials, 6(8), 3131-3142.
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3

APCVD Growth of Single-Layer MoS2

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Witec alpha 300 Raman spectrometer was used for acquiring all Raman and PL spectra with a grating of 1800 lines per mm and a 50× objective (0.7 NA) with a spot size of ∼1 μm. The resolution of the Raman spectrometer was 1.3 cm−1 and the applied power was 3.5 mW. For all the spectra, the excitation wavelength was 532 nm. The Lorentzian function was used for fitting the Raman plots. To measure the thickness of the flakes Park systems NX10 AFM was used, with a tip radius less than 10 nm, force constant of 42 N m−1, and 330 kHz frequency. The AFM micrographs were recorded in non-contact mode. The optical micrographs were recorded by using a Nikon Eclipse LV100ND microscope.
Single layer MoS2 was grown over 290 nm SiO2/Si in a two-zone atmospheric pressure CVD (APCVD). Sulphur (Sigma Aldrich, 99.98%) and molybdenum(iv) oxide (Alfa Aesar, 99%) was used as a precursor. 4 mg MoO3 was kept in an alumina crucible in the middle of zone one (825 °C) and 315 mg S was kept 35 cm upstream in zone two (285 °C). 100 SCCM Ar was used as a carrier gas and also for purging (15 min). The SiO2/Si was cleaned in acetone, isopropyl alcohol, and deionized water before loading into the growth chamber. Growth was carried out for 45 min in the presence of Ar atmosphere.
Basu N., Kumar R., Manikandan D., Ghosh Dastidar M., Hedge P., Nayak P.K, & Bhallamudi V.P. (2023). Strain relaxation in monolayer MoS2 over flexible substrate. RSC Advances, 13(24), 16241-16247.
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4

Ru/RGO Nanocomposite Characterization

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The morphological analysis of the as-obtained
nanocomposite was
performed using a scanning electron microscope (SEM; JEOL JSM-7610F
FEG-SEM) with EDS and HR-TEM (Titan TEM 300 kV) with the fast Fourier
transform pattern. A PANalytical Empyrean at 40 kV and 40 mA with
Cu Kα radiation and a Witec Alpha 300 Raman spectrometer (laser
wavelength of 532 nm) were used to investigate the sample crystallinity
and structure. The specific surface area and pore size distributions
of the as-obtained nanocomposites were determined using Quantachrome
Autosorb 06 at −196 °C under N2. The sample
was degassed at 250 °C for 4 h to remove atmospheric impurities.
The XPS ESCALab MKII was used to analyze the chemical composition
and chemical states of the Ru/RGO nanocomposite.
Bharath G, & Banat F. (2021). High-Grade Biofuel Synthesis from Paired Electrohydrogenation and Electrooxidation of Furfural Using Symmetric Ru/Reduced Graphene Oxide Electrodes. ACS Applied Materials & Interfaces, 13(21), 24643-24653.
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5

Raman Imaging of Blastocyst Lipids

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Raman imaging was performed with a WITec alpha300 Raman spectrometer. At the time of analysis, the embryos were thawed, fixed in 4% paraformaldehyde for 20 min, washed first in 0.4% PBS-PVP, and then, placed on a calcium fluoride slide in drops of 300 µL of 0.4% PBS-PVP. The blastocysts were measured with a laser line of 532 nm, an immersive objective (60×), and 600-grooves∙mm−1 grating. Step sizes equal to 3 µm and integration time of 0.5 s were applied. Raman images were generated for lipids (2,830 to 2,900 cm−1). Data preprocessing and analysis were performed with WITec Project Plus software. Preprocessing included cosmic ray spike removal and background subtraction (third-order polynomial). K-Means Clustering (KMC) analysis enabled the spectra to be grouped into classes, each of which represented the main blastocyst biochemical composition. Additionally, averaged spectra of each class were generated showing their characteristic spectral profile.
Arena R., Bisogno S., Gąsior Ł., Rudnicka J., Bernhardt L., Haaf T., Zacchini F., Bochenek M., Fic K., Bik E., Barańska M., Bodzoń-Kułakowska A., Suder P., Depciuch J., Gurgul A., Polański Z, & Ptak G.E. (2021). Lipid droplets in mammalian eggs are utilized during embryonic diapause. Proceedings of the National Academy of Sciences of the United States of America, 118(10), e2018362118.
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6

Raman Imaging of Microparticle Phases

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Raman imaging was used for the evaluation of the spatial distribution of hydrophilic and lipophilic phases in the microparticles. A WITec Alpha 300 Raman spectrometer was used to generate Raman spectra of the samples in the range of 10–3600 cm−1, with a spectral resolution of 3 cm−1. Raman spectrometer was connected with a confocal microscope, equipped with TrueSurface attachment and EMCCD detector for ultra-fast and sensitive imaging. The samples were irradiated with a focused laser beam at a power of 15 mW. The laser was emitting at the wavelength of 532 nm. The b-MPs were mapped with a spatial resolution of 5 µm. The measuring step was set to 1.67 µm, the accumulation time of a single spectrum was 0.5 s. Before Raman mapping, Raman spectra for all used raw materials were recorded in order to identify key marker bands for the identification of excipients in the investigated samples. No special sample preparation was needed and the applied Raman mapping technique was non-destructive for the samples. The collected Raman mapping data was analyzed by means of the Project Four 4.1 Plus software. The analysis based on the calculation of the integral value (area under the band) for the selected bands as well as chemometric analysis (k-means cluster analysis) were performed in order to describe a distribution of hydrophilic and lipophilic phase in the investigated- MPs.
Bertoni S., Albertini B., Ronowicz-Pilarczyk J., Calonghi N, & Passerini N. (2021). Solvent-Free Fabrication of Biphasic Lipid-Based Microparticles with Tunable Structure. Pharmaceutics, 14(1), 54.
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7

SERS Detection of DMMP Vapor

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SERS experiments for detecting DMMP in gas phase were conducted in a gas cell (2.7x10 -2 cm 3 ), where a gas stream, 10ml STP/min, containing 2.5 ppmV (14 mg/m 3 ) of DMMP in nitrogen was fed continuously. DMMP vapours were generated using a calibrated permeation tube as described in our previous work [9] (link). Two Raman equipments were used: the benchtop equipment described above (Alpha 300 Raman spectrometer, WITec) and a portable Raman BWTEK i-Raman pro system (6 cm -1 spectral resolution). In the latter case, an excitation wavelength of 785nm with a power laser of 280 mW (0.035 mW/µm 2 ), 1s of integration time and averaging 100 spectra were used for all the measurements. A schematic drawing together with a picture of the set-up with the portable Raman and the gas cell for measurements are presented in Fig. 1.
Lafuente M., Sanz D., Urbiztondo M., Santamaría J., Pina M.P, & Mallada R. (2020). Gas phase detection of chemical warfare agents CWAs with portable Raman. Journal of hazardous materials, 384.
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8

SERS Characterization and Analytical Enhancement

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An Alpha 300 Raman spectrometer of WITec was used with a confocal optical microscope (480nm as lateral spatial resolution, 2 cm -1 spectral resolution). Raman-SERS spectra were collected in backscattering geometry. Excitation of the samples was carried out with lasers 532nm, 633nm and 785 nm at room temperature and applying a irradiance of 0.13 mW/µm 2 , 0.67 mW/µm 2 and 0.88 mW/µm 2 , respectively.
The analytical enhancement factor (AEF) [14] (link), of the different substrates, was calculated according to equation (1). R6G was selected as probe molecule monitoring its C-C stretching mode displaced at 1512 cm -1 .
(
Where C Raman and C SERS are the R6G concentration in the Raman measurements and SERS conditions, respectively. And I Raman and I SERS are the normalized intensity values (cts•mW -1 •s -1 ) of the 1512 cm -1 band for normal Raman and SERS measurements, respectively.
A droplet of 2µL aqueous solution R6G 10 µM was deposited on the SERS substrate and dried under ambient conditions. The R6G spectrum of the SERS substrate was measured in ten different points of the dried droplet and the intensity of the peak at 1512 cm -1 was averaged. The normal Raman R6G spectrum was measured focusing the laser beam, 633 nm and 785 nm, in the aqueous solution R6G 1 mM. For the 532 nm laser line, the R6G droplet was dried before acquisition to avoid fluorescence effects.
Lafuente M., Sanz D., Urbiztondo M., Santamaría J., Pina M.P, & Mallada R. (2020). Gas phase detection of chemical warfare agents CWAs with portable Raman. Journal of hazardous materials, 384.
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