2300 stat plus

Manufactured by Yellow Springs Instruments

Sourced in United States

The 2300 Stat Plus is a compact and versatile lab instrument designed for electrochemical measurements. It provides precise control and monitoring of electrochemical parameters, enabling accurate data collection and analysis.

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Lab products found in correlation

Ezhcp 20k, by Merck Group (1 mentions) Synchron system, by Beckman Coulter (1 mentions) Ezhiasf 14k, by Merck Group (1 mentions) Nefa hr 2, by Fujifilm (1 mentions) Enzyme linked immunosorbent assay, by R&D Systems (1 mentions) K3 edta, by Sarstedt (1 mentions) Dca vantage analyzer, by Siemens (1 mentions) Reflotron plus, by Roche (1 mentions)

Close spelling variants of this product

YSI 2300 STAT PLUS Enzymatic Electrode-YSI analyzer YSI 2300 STAT Plus analyzer YSI 2300 Stat Plus YSI 2300 Stat Plus-D YSI 2300 STAT Plus Glucose and Lactate Analyzer Model 2300 Stat Plus YSI 2300 Stat Plus Glucose Analyzer YSI model 2300 Stat Plus YSI 2300 STAT Plus Glucose & Lactate Analyzer

8 protocols using 2300 stat plus

Whole-blood Glucose and Lactate Measurement

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Whole-blood glucose and lactate levels in the whole blood were measured using an automated glucose-lactate analyzer (2300 Stat Plus, Yellow Springs Instruments, USA).

Soya M., Matsui T., Shima T., Jesmin S., Omi N, & Soya H. (2018). Hyper-hippocampal glycogen induced by glycogen loading with exhaustive exercise. Scientific Reports, 8, 1285.

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Characterization of Metabolic Responses

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Blood samples were separated into plasma or serum at the time of collection and stored at −80°C until analysis. Plasma glucose was measured by the glucose oxidase method (2300STAT Plus, Yellow Springs Instruments, Yellow Springs, OH). Serum insulin and C-peptide were measured using commercial ELISA kits (#EZHIASF-14K and #EZHCP-20K, respectively, Millipore, St. Louis, MO). Serum triglycerides, total cholesterol, and HDL-cholesterol were measured at the Clinical Chemistry Laboratory of the Oklahoma Veterans Administration Hospital (Oklahoma City) using validated enzymatic assays (Synchron Systems, Beckman Coulter, Brea, CA). Serum non-esterified fatty acids (NEFA) were measured in with an enzymatic colorimetric assay (NEFA-HR2, Wako Chemicals, Richmond, VA). All assays were performed with appropriate standards and quality controls according to manufacturer’s directions.
The fasting concentrations of each analyte was calculated as the average of two blood samples collected at 10 and 2 minutes prior to meal ingestion. The concentrations of glucose, insulin, C-peptide, and NEFA from 0–180 minutes after the meal were used to calculate the total area under the curve (AUC) using the trapezoidal method, and the oral glucose insulin sensitivity index (15 ).

Short K.R., Pratt L.V, & Teague A.M. (2018). A single exercise session increases insulin sensitivity in normal weight and overweight/obese adolescents. Pediatric diabetes.

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Breast Milk Composition Analysis

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Based on their discretion, mothers chose the breast they felt could provide the most complete milk expression (though the right was encouraged). Furthermore, the mother was encouraged to completely empty the entire contents of a single breast for the analyses of breast milk insulin, glucose, leptin, IL-6 and TNF-α using an electric breast pump (Medela, Inc.) provided by the investigative team. Milk fat was separated from the aqueous phase by centrifugation with the resulting skimmed milk assayed using commercially available immunoassay kits for insulin, leptin, IL-6 and TNF-α as described by our group previously (7 (link)). Glucose was measured using the glucose oxidase method (2300 STAT Plus, Yellow Springs Instruments).

Fields D.A., George B., Williams M., Whitaker K., Allison D.B., Teague A, & Demerath E.W. (2017). Associations between human breast milk hormones and adipocytokines and infant growth and body composition in the first six-months of life. Pediatric obesity, 12(Suppl 1), 78-85.

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Automated Glucose-Lactate Analysis

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Blood glucose and lactate levels were measured using an automated glucose-lactate analyzer (2300 Stat Plus, Yellow Springs Instruments, United States).

Matsui T., Liu Y.F., Soya M., Shima T, & Soya H. (2019). Tyrosine as a Mechanistic-Based Biomarker for Brain Glycogen Decrease and Supercompensation With Endurance Exercise in Rats: A Metabolomics Study of Plasma. Frontiers in Neuroscience, 13, 200.

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Blood Sampling in Sprint Exercise

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Venous blood samples were taken in a standing position, at baseline in ambient conditions and immediately prior to and immediately following every sprint bout in the heat, resulting in seven individual samples (Baseline, Pre-exercise, Post Set 1, Post FSINT 1, Post Set 2, Post FSINT 2, Post Set 3) at which times participants were stationary. Whole blood was initially analyzed for lactate and glucose (Yellow Springs Instrument, 2300 STAT plus, Yellow Springs Instruments Inc., USA) and then aliquots were dispensed into K₃-EDTA, LH and serum tubes (Sarstedt Ltd, Leicester, United Kingdom). The aliquots were then centrifuged at 4000 g for 10 min at 4°C. After centrifuging the supernatant was removed and then frozen at −80°C until the analyses were performed.
Changes in blood, plasma and red cell volume were calculated from the mean hemoglobin concentration (cyanomethaemoglobin method, Cecil Instruments, Cambridge, United Kingdom, measured in duplicate) and the mean haematocrit (Micocentrifugation, Hawksley, Sussex, United Kingdom, in triplicate) using the standard equations (Dill and Costill, 1974 (link)).
Serum concentrations of cortisol and plasma prolactin were determined via enzyme-linked immunosorbent assays (R&D Systems, Abingdon, United Kingdom). The intra-assay coefficient of variation for the cortisol and prolactin ELISAs were 5.4 and 7.7% respectively.

Sunderland C., Stevens R., Everson B, & Tyler C.J. (2015). Neck-cooling improves repeated sprint performance in the heat. Frontiers in Physiology, 6, 314.

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Breast Milk Metabolic Biomarkers Analysis

Cited 3 times

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Analyses of human milk samples were conducted by the University of Oklahoma Health Sciences Centre Metabolic Research Program Laboratory. Milk fat was separated from the aqueous portion by centrifugation in preparation to assay samples of skimmed milk. Glucose was measured using the glucose oxidase method (2300 STAT Plus, Yellow Springs Instruments, Yellow Springs, OH). Insulin, leptin, adiponectin, CRP, and IL-6 were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s protocol, as previously reported (Fields and Demerath 2012 (link); Whitaker et al. 2017 (link); Sadr Dadres et al. 2019 (link)). Interassay variability was 5.6% for glucose, 6.2% for insulin, 5.1% for leptin, 5.9% for adiponectin, 5.5% for CRP, and 12.8% for IL-6. Intra-assay variability was 10.2% for glucose, 5.1% for insulin, 5.6% for leptin, 2.0% for adiponectin, 4.7% for CRP, and 9.1% for IL-6. Breast milk analyte concentrations of glucose (mg/dl), insulin (pg/ml), leptin (pg/ml), adiponectin (ng/ml), CRP (ng/ml), and IL-6 (pg/ml) were recorded at 1- and 3-month lactation. Adiponectin measurements in the 3-month samples were limited to a subset of 125 participants due to cost constraints.

Eckart E.K., Peck J.D., Kharbanda E.O., Nagel E.M., Fields D.A, & Demerath E.W. (2021). Infant sex differences in human milk intake and composition from 1- to 3-month post-delivery in a healthy United States cohort. Annals of human biology, 48(6), 455-465.

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Evaluating Insulin Sensitivity Using iHOMA2

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Venous blood samples were collected in the morning following an overnight fast. Glycated hemoglobin (HbA1c) was measured on whole blood at the time of collection using a Siemens DCA Vantage analyzer (Tarrytown, NY). After centrifugation, aliquots of plasma and serum were stored at -80°C until analysis. Plasma glucose was measured by the glucose oxidase method (2300STAT Plus, Yellow Springs Instruments, Yellow Springs, OH). Serum insulin was measured using a chemiluminescent enzyme-linked immunosorbent assay (ELISA) from ALPCO (#80-INSHU-CH10, Salem, NH). Insulin sensitivity was calculated using glucose and insulin concentrations with the revised integrated homeostatic model of assessment (iHOMA2) [29 (link)].

Short K.R., Chadwick J.Q., Cannady T.K., Branam D.E., Wharton D.F., Tullier M.A., Thompson D.M, & Copeland K.C. (2018). Using financial incentives to promote physical activity in American Indian adolescents: A randomized controlled trial. PLoS ONE, 13(6), e0198390.

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Blood Lactate and Creatine Kinase Analysis

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To determine blood lactate and CK concentrations, aliquots of blood were obtained from a finger-prick sample. Blood lactate was directly analysed using an automated analyser (2300 STAT plus, Yellow Springs Instruments Inc., Ohio, USA) in duplicate. A 32 µl sample of blood was pipetted onto a CK Test Strip (Reflotron® Plus, Roche Diagnostics, UK) and analysed in duplicate using a commercially available Reflotron CK Assay (Reflotron® Plus, Roche Diagnostics, UK). Self-ratings of perceived quadriceps muscle soreness were assessed using a 10 point scale ranging from 1 (not sore) to 10 (very, very sore) (35) while the participants stood.

Anderson D., Nunn J, & Tyler C.J. (2018). Effect of Cold (14° C) vs. Ice (5° C) Water Immersion on Recovery From Intermittent Running Exercise. Journal of strength and conditioning research, 32(3).

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