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Glutathione peroxidase gpx

Manufactured by Cayman Chemical
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Glutathione peroxidase (GPx) is an enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to their corresponding alcohols. It plays a crucial role in the cellular defense against oxidative stress by converting harmful peroxides into less reactive species.

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Lab products found in correlation

Malondialdehyde mda, by Abcam (2 mentions) Tnf α, by R&D Systems (2 mentions) Advia 120, by Bayer (2 mentions) Alanine transaminase alt, by Merck Group (1 mentions) Aspartate aminotransferase ast, by Merck Group (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Mlb00c, by R&D Systems (1 mentions) Mta00b, by R&D Systems (1 mentions) Il 1β, by R&D Systems (1 mentions)

Close spelling variants of this product

Glutathione peroxidase (GPx) assay kit Glutathione peroxidase

4 protocols using glutathione peroxidase gpx

1

Immune and Biochemical Markers Evaluation

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Blood samples were collected into non-heparinized and K3-EDTA vacuum
tubes (Becton Dickinson Vacutainer Systems) to determine numbers of immune
cells, including leukocytes, lymphocytes, and monocytes, after 3 and 4 weeks.
Immune cell counts were determined using an automatic blood analyzer (ADVIA120;
Bayer, Leverkusen, Germany). ELISA was used to quantify serum concentrations of
tumor necrosis factor (TNF)-α (R&D Systems,
Minneapolis, MN, USA), alanine transaminase (ALT; Sigma-Aldrich, St. Louis, MO,
USA), aspartate aminotransferase (AST; Sigma-Aldrich, St. Louis, MO, USA),
superoxide dismutase (SOD; Cohesion Biosciences, USA), malondialdehyde (MDA;
Abcam, Cambridge, UK), and glutathione peroxidase (GPx; Cayman Chemical, Ann
Arbor, MI, USA).
Kang K.S., Shin S, & Lee S.I. (2020). N-acetylcysteine modulates cyclophosphamide-induced immunosuppression, liver injury, and oxidative stress in miniature pigs. Journal of Animal Science and Technology, 62(3), 348-355.
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2

Blood Sample Analysis Protocol

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At the end of the experiment (21st day), blood samples were collected and analysed according to our standard protocol50 (link). Briefly, blood samples were collected from all pigs via jugular venipuncture 6 h after the challenge into a non-heparinised K3EDTA vacuum tube (Becton Dickinson Vacutainer Systems, Franklin Lakes, NJ, USA) to obtain serum and whole blood. Leukocyte, lymphocyte, and monocyte counts were determined using an automatic blood analyser (ADVIA 120; Bayer, Leverkusen, Germany).
The whole blood samples were subsequently centrifuged at 3,000 × g for 15 min at 4 °C, and the serum was harvested. Thereafter, the samples were frozen and stored at −20 °C until further analysis. The levels of serum TNF-α (R&D Systems, Minneapolis, MN, USA), IL-6 (R&D Systems), superoxide dismutase (SOD) (Cohesion Biosciences, London, UK), glutathione peroxidase (GPx) (Cayman Chemical, Ann Arbor, MI, USA), and malondialdehyde (MDA) (Abcam, Cambridge, UK) were determined using an ELISA kit.
Lee S.I, & Kang K.S. (2017). Function of capric acid in cyclophosphamide-induced intestinal inflammation, oxidative stress, and barrier function in pigs. Scientific Reports, 7, 16530.
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3

Enzymatic Activities in Surgical Granulation Tissue

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On day 20 after surgery, the granulation tissues (200 mg) were homogenized in Tris buffer. The tissue homogenates were centrifuged at 6,000 rpm for 20 minutes at 4°C and the supernatant was employed for further assessment of the enzymatic activities. Commercial kits (Cayman, Ann Arbor, MI, USA) were applied to measure the activity of catalase (CAT) (Item No 707002; Cayman), glutathione peroxidase (GPx) (Item No 703102; Cayman), and superoxide dismutase (SOD) (Item No 706002; Cayman) in tissue homogenates, according to the vendor’s protocol.
Rouhollahi E., Moghadamtousi S.Z., Hajiaghaalipour F., Zahedifard M., Tayeby F., Awang K., Abdulla M.A, & Mohamed Z. (2015). Curcuma purpurascens BI. rhizome accelerates rat excisional wound healing: involvement of Hsp70/Bax proteins, antioxidant defense, and angiogenesis activity. Drug Design, Development and Therapy, 9, 5805-5813.
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4

Humerus Biomarker Quantification Protocol

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The humerus of each mouse was prepared by first gently removing adhering tissue with a scalpel. The humerus was then placed in 500 µL of PBS and homogenized for fifteen seconds. The homogenate was centrifuged at 14,000× g at 4 °C for ten minutes and the supernatant was collected and stored at −80 °C until used for biomarker assays.
Commercial kits were used according to manufacturers’ instructions to measure bone homogenate biomarkers. C-terminal telopeptide of type I collagen (CTX) was measured with an enzyme-linked immunosorbent assay (ELISA) kit (MBS453660, MyBioSource, Inc., San Diego, CA, USA) to determine bone resorption. Glutathione peroxidase (GPx) (#703102, Cayman Chemical, Ann Arbor, MI, USA), and catalase (CAT) (Cayman, #707002) were measured to determine the antioxidant capacity. Tumor necrosis factor α (TNF-α) (R&D Systems, MTA00B) and interleukin-1 beta (IL-1β) (R&D Systems, MLB00C) were measured with ELISA kits to determine inflammation.
Sakaki J., Melough M., Lee S.G., Kalinowski J., Koo S.I., Lee S.K, & Chun O.K. (2018). Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice. Nutrients, 10(11), 1671.
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