Total superoxide dismutase t sod

DMY (purity > 98%) was purchased from Shanghai Yuanye Bio-technology Co., Ltd. (Shanghai, China). Isopropyl myristate, polyoxyethylene hydrogenated castor oil, and diethylene glycol monoethyl ether were provided by Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Cycloheximide (CYC) was obtained from Chengdu Huaxia Chemical Reagent Co., Ltd. (Chengdu, China). Total superoxide dismutase (T-SOD) and malondialdehyde (MDA) kits were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other chemicals were of analytical or high-performance liquid chromatography (HPLC) grade.

All the solvents used for extract were of analytical grade (Baishi Chemical Co. Ltd., Tianjin, P. R. China), and water was double distilled. Metformin hydrochloride tablets were purchased from Harbin Tongyitang Pharmaceutical Co., Ltd (China). Antibodies to GRP78, ß-actin, phospho-IER1α, phosphor-PERK, phospho-e1F2α, XBP1s, IER1α, PERK, and e1F2α were obtained from Cell Signaling Technology (Danvers, MA, USA). The secondary antibody was purchased from Boster Biological Technology Co., Ltd (China). Electrophoresis reagents, including Bis-Tris gels, running buffer, and poly (vinylidene fluoride) (PVDF) membrane, were obtained from Invitrogen (Carlsbad, CA, USA). ECL was bought from GE Healthcare (UK). Kit of low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), blood plasma free fatty acid (FFA), total cholesterol (TC), triacylglyceride (TG), fructosamine, hexokinase (HK), glycogen, catalase (CAT), total Superoxide Dismutase (T-SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) were purchased from Nanjing Jiancheng Bioengineering Institute (China). Pierce TMT® Mass Tagging Kits and Reagents were purchased from Thermo Science Pierce (Rockford, USA).

RSV (98%) was bought from Xian SenZhuo Technology Co., Ltd. (Xian, China). Acetic acid (AC) was bought from Wuhan Jiangbei Chemical Reagent Co., Ltd. (Wuhan, China). Indomethacin was bought from Shanxi SanJin Pharmaceutical Co., Ltd. (Shanxi, China). Xylene was bought from Hubei Shashi Chemical Reagent Co., Ltd. (Hubei, China). Dexamethasone (DXM) was bought from Tianjin Pharmaceutical Group Co., Ltd. (Tianjin, China). Carrageen was bought from the Sigma Company (USA); nitrogen monoxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), total superoxide dismutase (T-SOD), prostaglandin E2 (PEG₂), and total protein (TP) kits were all bought from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).