Fmoc protected α amino acids

Manufactured by Chem-Impex

Fmoc protected α-amino acids are commonly used as building blocks in solid-phase peptide synthesis. They provide a temporary protecting group for the amino group of amino acids, allowing for the selective coupling of amino acids during the peptide synthesis process.

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Lab products found in correlation

Rink amide resin, by Chem-Impex (6 mentions) Lyophilizer, by Labconco (2 mentions) Breeze 2 hplc system, by Waters Corporation (1 mentions) 4700 proteomics analyzer, by Thermo Fisher Scientific (1 mentions) Inova 400, by Agilent Technologies (1 mentions) Breeze 2 hplc system, by Labconco (1 mentions)

6 protocols using fmoc protected α amino acids

Fmoc-protected α-amino acid peptide synthesis

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Fmoc–protected α-amino acids and Rink amide resin used for γ-AApeptide synthesis were purchased from Chem-Impex International, Inc. All chemicals and solvents used were purchased from Aldrich or Fisher and were used without further purification.

Bolarinwa O., Li C., Khadka N., Li Q., Wang Y., Pan J, & Cai J. (2020). γ-AApeptides–based Small Molecule Ligands That Disaggregate Human Islet Amyloid Polypeptide. Scientific Reports, 10, 95.

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Solid-Phase Synthesis of Sulfono-γ-AApeptides

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All reagents and solvents were purchased from Fisher or Aldrich and used without further purification. Fmoc protected α-amino acids and Rink-amide resin (0.6 mmol/g, 200‒400 mesh) were purchased from Chem-Impex International, Inc. The sulfono-γ-AApeptide building block synthesized on solid support Rink-amide resin as previously reported^{31 (link),32 (link)}. Full details of the chemical synthesis and purification are given in the Supplementary Methods.

Teng P., Zheng M., Cerrato D.C., Shi Y., Zhou M., Xue S., Jiang W., Wojtas L., Ming L.J., Hu Y, & Cai J. (2021). The folding propensity of α/sulfono-γ-AA peptidic foldamers with both left- and right-handedness. Communications Chemistry, 4, 58.

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Solid-Phase Peptide Synthesis and Purification

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All reagents and solvents were purchased from Fisher or Aldrich and used without further purification. Fmoc protected α-amino acids and Rink-amide resin (0.6 mmol/g, 200-400 mesh)
were purchased from Chem-Impex International, Inc. Solid-phase syntheses of the compounds were carried out in the peptide synthesis vessel on a Burrell Wrist-Action shaker. The products were analyzed and purified on a Waters Breeze 2 HPLC system installed with both analytic module
(1 mL/min) and preparative module (16 mL/min), by employing a method using 5-100% linear gradient of solvent B (0.1% TFA in acetonitrile) in solvent A (0.1% TFA in water) over 40 min, followed by 100% solvent B over 10 min. The desired fractions were collected and lyophilized on a Labconco lyophilizer. High-resolution MS was conducted on an Agilent 6540 LC/QTOF system.
1 HNMR spectra were acquired with the WET solvent suppression at Varian 600 MHz.

Teng P., Zheng M., Cerrato D.C., Shi Y., Zhou M., Xue S., Jiang W., Wojtas Ł., Ming L., Hu Y., & Li Q. (2020). The Folding Propensity of α/Sulfono-γ-AA Peptidic Foldamers with Both Left- and Right-Handedness.

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Solid-Phase Peptide Synthesis Protocol

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Rink-amide resin (0.64 mmol g⁻¹, 200–400 mesh) and Fmoc protected α-amino acids were purchased from Chem-impex Int’l Inc. Solvents, coupling reagents, and other chemicals of reagent grade were purchased from either Fisher Scientific or Sigma-Aldrich. Column chromatography was carried out with silica gel (200–300 mesh). All compounds were synthesized by using solid-phase peptide synthesis. The compound was synthesized in a peptide reaction vessel clamped on a Burrell wrist-action shaker and purified on a High-Performance Liquid Chromatography (HPLC) system with both analytical and preparative functions. The final product’s molecular weight was confirmed by using a Bruker AutoFlex MALDI-TOF mass spectrometer, and the desired final pure products were dried on a Labcono lyophilizer.

Wei L., Gao R., Wang M., Wang Y., Shi Y., Gu M, & Cai J. (2021). Dimeric lipo-α/sulfono-γ-AA hybrid peptides as broad-spectrum antibiotic agents. Biomaterials science, 9(9), 3410-3424.

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Synthesis and Purification of Heterogeneous Peptides

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All Fmoc-protected α-amino acids and Rink-amide resin (0.7 mmol/g, 200–400 mesh) were purchased from Chem-Impex International, Inc. Other solvents and reagents were purchased from either Sigma-Aldrich or Fisher Scientific and used without further purification. Solid-phase synthesis of 1:1 α/sulfono-γ-AA peptides were carried out in the peptide synthesis vessel on a Burrell Wrist-Action shaker. The sequences were purified on a Waters Breeze 2 HPLC system, and desired fractions were collected and lyophilized on a Labcono lyophilizer. The molecular weight of the heterogeneous peptides was obtained on an Applied Biosystems 4700 Proteomics Analyzer.

She F., Nimmagadda A., Teng P., Su M., Zuo X, & Cai J. (2016). Helical 1:1 α/Sulfono-γ-AA Heterogeneous Peptides with Antibacterial Activity. Biomacromolecules, 17(5), 1854-1859.

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Solid-Phase Peptide Synthesis Protocol

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All Fmoc protected α-amino acids and Rink-amide resin (0.7 mmol/g, 200–400 mesh) were purchased from Chem-Impex International, Inc. Solvents and other reagents were purchased from either Sigma-Aldrich or Fisher Scientific and were used without further purification. Solid-phase synthesis of the compounds was carried out in the peptide synthesis vessel on a Burrell wrist-action shaker. The products were purified on a Waters Breeze 2 HPLC system, and lyophilized on a Labconco lyophilizer. The purity of the compounds was determined to be >95% by analytical HPLC (1 mL/min flow, 5–100% linear gradient of solvent B (0.1% TFA in acetonitrile) in A (0.1% TFA in water) over 40 min was used). The NMR spectra were obtained on a Varian Inova 400 instrument.

Teng P., Huo D., Nimmagadda A., Wu J., She F., Su M., Lin X., Yan J., Cao A., Xi C., Hu Y, & Cai J. (2016). Small Antimicrobial Agents Based on Acylated Reduced Amide Scaffold. Journal of medicinal chemistry, 59(17), 7877-7887.

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