Primary antibodies of cGMP-dependent protein kinase 1 (cGK I), cGK II, proliferating cell nuclear antigen (PCNA), MAPK phosphatase-1 (MKP-1), p-Erk1/2, p-p38, p21Cip1, and p27Kip1 were obtained from Santa Cruz Biotechnology (San Diego, CA, USA). Anti-mouse secondary antibody conjugated with Alexa Fluor® 647, 488 and ProLong Gold antifade reagent with 4’, 6-diamidino-2-phenylindole (DAPI) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). A71915 and Rp-8-Br-cGMPS (hereafter indicated as Rp) were purchased, respectively, from Bachem (Torrance, CA, USA) and EMD Calbiochem (San Diego, CA, USA). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). A creatinine kit was purchased from BioAssay System (Hayward, CA, USA). A microalbumin assay kit was purchased from Bethyl Laboratories (Montgomery, TX, USA). RNase-free DNase was obtained from Qiagen (Valencia, CA, USA). A multiplex kit for mouse cytokine assay was purchased from Millipore (Billerica, MA, USA). A cGK activity assay kit was purchased from MBL International (Woburn, MA, USA). A cGMP assay kit was purchased from Assay Designs (Ann Arbor, MI, USA). All other chemicals were of reagent grade.
Creatinine kit
Manufactured by BioAssay Systems
Sourced in United States
The Creatinine Kit is a laboratory product designed for the quantitative determination of creatinine levels in biological samples. It provides a reliable and efficient method for measuring creatinine concentrations.
Automatically generated - may contain errors
Lab products found in correlation
Prolong gold antifade reagent with 4 6 diamidino 2 phenylindole dapi, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Mkp 1, by Santa Cruz Biotechnology (2 mentions)
Rnase free dnase, by Qiagen (2 mentions)
Microalbumin assay kit, by Fortis Life Sciences (2 mentions)
Multiplex kit for mouse cytokine assay, by Merck Group (2 mentions)
A71915, by Bachem (2 mentions)
Rp 8 br cgmps, by Bachem (2 mentions)
P21cip1, by Santa Cruz Biotechnology (2 mentions)
P erk1 2, by Santa Cruz Biotechnology (2 mentions)
P27kip1, by Santa Cruz Biotechnology (2 mentions)
Cgk activity assay kit, by MBL Life Science (2 mentions)
Dnase 1, by Merck Group (1 mentions)
Collagenase 4, by Thermo Fisher Scientific (1 mentions)
Gallios flow cytometer, by Beckman Coulter (1 mentions)
3 protocols using creatinine kit
1
Investigating cGMP-dependent Signaling Pathways
2
Molecular Profiling of Cellular Signaling
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Primary antibodies of cGMP‐dependent protein kinase 1 (cGK I), cGK II, proliferating cell nuclear antigen (PCNA), MAPK phosphatase‐1 (MKP‐1), p‐Erk1/2, p‐p38, p21Cip1, and p27Kip1 were obtained from Santa Cruz Biotechnology (San Diego, CA, USA). Anti‐mouse secondary antibody conjugated with Alexa Fluor 647, 488 and ProLong Gold antifade reagent with 4′, 6‐diamidino‐2‐phenylindole (DAPI) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). A71915 and Rp‐8‐Br‐cGMPS (hereafter indicated as Rp) were purchased, respectively, from Bachem (Torrance, CA, USA) and EMD Calbiochem (San Diego, CA, USA). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). A creatinine kit was purchased from BioAssay System (Hayward, CA, USA). A microalbumin assay kit was purchased from Bethyl Laboratories (Montgomery, TX, USA). RNase‐free DNase was obtained from Qiagen (Valencia, CA, USA). A multiplex kit for mouse cytokine assay was purchased from Millipore (Billerica, MA, USA). A cGK activity assay kit was purchased from MBL International (Woburn, MA, USA). A cGMP assay kit was purchased from Assay Designs (Ann Arbor, MI, USA). All other chemicals were of reagent grade.
3
Measuring Immune Cells in Kidney Samples
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Commercially available enzyme-linked immunosorbent assays (R&D Systems; Millipore, Temecula, CA) were used to measure plasma levels (n = 7–8/group) of sFlt-1 and sEng. Plasma creatinine was measured with the creatinine kit from BioAssay Systems.
Fresh kidneys (n = 5/group) were minced with a razor blade, followed by digestion with 0.125 U DNaseI (Sigma) and 1 mg/mL collagenase IV (Invitrogen) for 45 min at 37 °C. After centrifugation, the single cell suspension was layered over a Ficoll-Hypaque gradient to yield the cells of interest [15 ]. T regulatory cells were identified with anti-mouse transcription factor forkhead box P3 (FOXP3) conjugated to allophycocyanin (RnD Systems), and T helper 17 cells were identified with anti-mouse retinoic orphan receptor-gamma (RORγ) conjugated to peridinin-chlorophyll-protein (BD Pharmingen) as previously described [15 ]. All cells were analyzed using a Beckman Coulter Gallios Flow Cytometer, which acquired 25,000 events per sample.
Fresh kidneys (n = 5/group) were minced with a razor blade, followed by digestion with 0.125 U DNaseI (Sigma) and 1 mg/mL collagenase IV (Invitrogen) for 45 min at 37 °C. After centrifugation, the single cell suspension was layered over a Ficoll-Hypaque gradient to yield the cells of interest [15 ]. T regulatory cells were identified with anti-mouse transcription factor forkhead box P3 (FOXP3) conjugated to allophycocyanin (RnD Systems), and T helper 17 cells were identified with anti-mouse retinoic orphan receptor-gamma (RORγ) conjugated to peridinin-chlorophyll-protein (BD Pharmingen) as previously described [15 ]. All cells were analyzed using a Beckman Coulter Gallios Flow Cytometer, which acquired 25,000 events per sample.
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