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Airtight chamber

Manufactured by Embrient Inc
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The Airtight Chamber is a laboratory equipment designed to provide a controlled and sealed environment. Its core function is to maintain an enclosed space with precise control over air pressure and gas composition. This chamber is suitable for various scientific and industrial applications that require a regulated atmospheric condition.

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Lab products found in correlation

L glucose, by Thermo Fisher Scientific (1 mentions) Oxymini fibre optic oxygen meter, by World Precision Instruments (1 mentions) Pc12 cells, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Airtight modular incubator chamber

5 protocols using airtight chamber

1

Hypoxic Exposure of Pulmonary Smooth Muscle

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Pulmonary arterial smooth muscle cells were exposed to hypoxia ex vivo by placing cells in an airtight chamber (Billups‐Rothenberg, Del Mar, CA) gassed with 4% O2; 5% CO2 or 1% O2; 5% CO2. The chamber was flushed prior to beginning exposure and again at 48 h. The chamber and the nonhypoxic controls (20% O2; 5% CO2) were placed in an incubator at 37°C for 60 h. Initial experiments were performed using a hand‐held oxygen monitor (Model 5577; Hudson RCI) to assure that the chamber was able to sustain the desired level of hypoxia for a minimum of 48 h.
Walker J., Undem C., Yun X., Lade J., Jiang H, & Shimoda L.A. (2016). Role of Rho kinase and Na+/H+ exchange in hypoxia‐induced pulmonary arterial smooth muscle cell proliferation and migration. Physiological Reports, 4(6), e12702.
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2

Oxygen-Glucose Deprivation Neuronal Injury Model

Cited 1 time
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Cells in control group were washed with phosphate buffered saline (PBS) and incubated in neurobasal medium in a humidified atmosphere of 95% air and 5% CO2 at 37°C. The exposure of cells to OGD was performed as we described before (Wu, et al., 2010 (link)). Briefly, neurobasal-A medium that did not contain L-glucose (GIBCO) was bubbled with 100% N2 for 30 min. Cells were washed with PBS once and 2 ml/well of the neurobasal-A medium was added to the cells. These plates were immediately placed in an air-tight chamber (Billups-Rothenberg, Inc., Del Mar, CA) gassed with 100% N2 for 10 min. The oxygen content in the outlet of the chamber was monitored with a DatexTM infrared analyzer (Capnomac, Helsinki, Finland) and reached 2% at 3 – 5 min after the onset of gassing. After closure of the inlet and outlet of the chamber, the chamber was kept at 37°C for 1 h. After confirming that the oxygen content in the chamber was still lower than 2% at the end of the OGD period, the chamber was opened and glucose, B-27 supplement and L-glutamine (500 μM) was added to make the final glucose concentration in the buffer at 4.5 g/l. The plates were kept for 6 h (Western blotting) or 20 h (Western blotting and LDH release assay) in a humidified atmosphere of 95% air and 5% CO2 at 37°C.
Cheng A., Lu Y., Huang Q, & Zuo Z. (2019). Attenuating oxygen-glucose deprivation-caused autophagosome accumulation may be involved in sevoflurane postconditioning-induced protection in human neuron-like cells. European journal of pharmacology, 849, 84-95.
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3

Enzymatic Hypoxia Induction Protocol

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Hypoxia was induced by enzymatic oxygen depletion using 4 U/ml glucose oxidase and 120 U/ml catalase as previously described [18 (link)]. For this study, the concentration of hypoxia-inducing enzymes was optimised after measuring oxygen levels in cell culture medium using the OxyMini fibre optic oxygen meter (World Precision Instruments, USA, Sarasota) and OxyMicro Software v.00 04/2003. After hypoxia induction, cell culture plates were put in an airtight chamber (Billups-Rothenberg, Del Mar, CA, USA), flushed with nitrogen until the oxygen concentration was < 1% and kept in the incubator at 37 °C for 24 h.
Smit K.F., Oei G.T., Konkel M., Augustijn Q.J., Hollmann M.W., Preckel B., Patel H.H, & Weber N.C. (2019). Plasma from Volunteers Breathing Helium Reduces Hypoxia-Induced Cell Damage in Human Endothelial Cells—Mechanisms of Remote Protection Against Hypoxia by Helium. Cardiovascular Drugs and Therapy, 33(3), 297-306.
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4

Hypoxia Induction in PC12 Cells

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The PC12 cells used in this study were purchased from the American Type Culture Collection (ATCC). The cells were maintained in DMEM with 10% foetal bovine serum (HyClone) at 37°C in a 5% CO2 incubator. PC12 cells were incubated in sugar‐free medium and then placed in an airtight chamber (Billups Rothenberg) flushed with a mixed gas of 95% N2/5% CO2 for 15 min. The chamber was kept at 37°C for 1 h. Control PC12 cells were incubated with sugar‐free medium and placed in the incubator. The cells are restored to their normal culture conditions after the hypoxia is over.
Ma X., Zhang M., Yan R., Wu H., Yang B, & Miao Z. (2021). β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia. Journal of Cellular and Molecular Medicine, 25(24), 11300-11309.
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5

Cortical Cell Hypoxia-Reoxygenation Culture

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On the 7th day of culturing primary cortical cells in vitro, the normal medium was discarded, and a sugar-free and serum-free Dulbecco's Modified Eagle Medium (DMEM) was added after washing three times in PBS. The plate was then placed in an air-tight chamber (Billups-Rothenberg, USA), flushed with an anaerobic gas mixture (95% N 2 and 5% CO 2 ) to remove oxygen, and then incubated at 37 °C for 90 min. The DMEM was discarded, replaced with a neurobasal medium containing 2% B-27 supplement, 0.5 mM glutamine, and 50 U/mL penicillin/streptomycin, and then the plates were placed back in the incubator (37 °C, 5% CO 2 ) for culture and reoxygenation for 24 h (Wu et al., 2018) .
Wang B., Ma L., Liu L., Qin J., Li T., Bu K., Li Z., Lu H., Song X., Cao Y., Cui J., Wang Q., Yuan S., Liu X, & Guo L. (2022). Receptor-Interacting Protein 3/Calmodulin-Dependent Kinase II/Proline-Rich Tyrosine Kinase 2 Pathway is Involved in Programmed Cell Death in a Mouse Model of Brain Ischaemic Stroke. Neuroscience, 506.
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