Mrc 5

Manufactured by European Collection of Authenticated Cell Cultures

Sourced in United Kingdom

The MRC-5 is a cell line derived from normal human fetal lung tissue. It is a well-established substrate for the propagation of various viruses and is commonly used in the development and production of vaccines.

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Lab products found in correlation

Peo1 10032308, by European Collection of Authenticated Cell Cultures (1 mentions) Thoma hemocytometer, by Marienfeld (1 mentions) Mrc 5 ccl 171, by American Type Culture Collection (1 mentions) Skov3, by European Collection of Authenticated Cell Cultures (1 mentions) Skov3 htb 77, by American Type Culture Collection (1 mentions) Nuli 1, by American Type Culture Collection (1 mentions) Panc 1, by European Collection of Authenticated Cell Cultures (1 mentions) Saos2, by European Collection of Authenticated Cell Cultures (1 mentions) Molt 4, by European Collection of Authenticated Cell Cultures (1 mentions) Mcf 7, by European Collection of Authenticated Cell Cultures (1 mentions) A2780, by European Collection of Authenticated Cell Cultures (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions)

4 protocols using mrc 5

Culturing Human Ovarian Cancer and Fibroblast Cells

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Two human ovarian cancer cell lines (SKOV3 and PEO1) and a normal human fibroblast MRC-5 cell line were used. The SKOV3 (HTB-77) and MRC-5 (CCL-171) cells were purchased from the American Type Culture Collection (ATCC). The PEO1 (10032308) cells were obtained from the European Collection of Authenticated Cell Cultures (ECACC).
The SKOV3 cells were cultured in McCoy’s 5A medium, the PEO1 cells in RPMI + GlutaMAX medium, and the MRC-5 cells in DMEM + GlutaMAX. All the media contained 10% heat-inactivated FBS and 1% v/v penicillin/streptomycin solution. The cells were cultured at 37 °C under 5% carbon dioxide and 95% humidity. They were passaged after reaching about 85% confluence. The Trypan Blue exclusion test was used for the evaluation of cell viability. A Thoma hemocytometer (Superior Marienfeld, Lauda-Königshofen, Germany) was used for cell counting.

Furdak P., Pieńkowska N., Kapusta I., Bartosz G, & Sadowska-Bartosz I. (2023). Comparison of Antioxidant and Antiproliferative Effects of Various Forms of Garlic and Ramsons. Molecules, 28(18), 6512.

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Culturing MRC-5 and NuLi-1 Cell Lines

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MRC-5 (human lung fibroblast cell line) cells were obtained from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK) and NuLi-1 (human airway epithelial cell line) cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The cells were cultured following the manufacturer’s protocol. These cell line cultures have intrinsic alterations compared to cells in humans and to cells in patients with an ongoing pathogenesis.

Arellano-Orden E., Calero Acuña C., Sánchez-López V., López Ramírez C., Otero-Candelera R., Marín-Hinojosa C, & López Campos J. (2022). Cellular mechanisms involved in the pathogenesis of airway remodeling in chronic lung disease. European Clinical Respiratory Journal, 9(1), 2097377.

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Characterization of Human Cell Lines

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Selected human tumour and non-tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and MRC-5 (non-tumour lung fibroblasts) were purchased from European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK) and cultured according to the provider's culture method guidelines. All cell lines were maintained at 37 °C in a humidified 5% carbon dioxide and 95% air incubator. The cells in low passage number (non-tumour primary cell line MRC-5 was used for a maximum of 10 passages and cancer cell lines were used for a maximum of 20 passages) and in an exponential growth phase were used for this study.

Ritomská A., Koutova D., Křoustková J., Královec K., Muthná D., Kuneš J., Nováková L., Havelek R, & Cahlíková L. (2023). Design of semisynthetic derivatives of the Amaryllidaceae alkaloid ambelline and exploration of their in vitro cytotoxic activities. Saudi Pharmaceutical Journal : SPJ, 31(8), 101684.

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Primary Lung Fibroblast Culture

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Primary normal human fibroblasts from the lung, MRC-5, were obtained from the European Collection of Authenticated Cell Cultures (ECACC, UK). MRC-5 cells were cultured in Basal Medium Eagle (BME) (SH30157.01, HyClone) supplemented with 10% fetal bovine serum (FBS) (S1820-500, Biowest) and penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO₂.

Ojima M., Ito A., Usami N., Ohara M., Suzuki K, & Kai M. (2021). Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams. Scientific Reports, 11, 7001.

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