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S 520 scanning microscope

Manufactured by Hitachi
Sourced in Germany

The S-520 scanning microscope is a tool designed for high-resolution imaging of samples. It utilizes an electron beam to scan the surface of a specimen, producing detailed images that can reveal the fine structure and topography of the sample.

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2 protocols using s 520 scanning microscope

1

Scanning Electron Microscopy of Hydrated Cement

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Prior to analysis by SEM,
the samples were pretreated. The 28-day
finely hydrated cement samples were dispersed onto ultrasmooth carbon
tape and then sputter-coated with a gold–palladium alloy under
a flowing argon atmosphere. Once the samples were ready, their morphology
was captured visually under a Hitachi S-520 scanning microscope with
20 kV acceleration voltage.
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2

Cell Culture and Characterization Protocol

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5% CO2 incubators at 37 degrees (Forma, USA), a disposable 10 mL syringe (Shanghai Misawa Medical Industry Co., Ltd.), Petri dishes, 24 and 6-well plate (Falcon, USA), centrifuge tubes (Falcon, USA), a centrifuge (Biofuge, Germany), low-temperature (refrigerated) high-speed centrifuges (Biofuge, Germany), an inverted phase contrast microscope (Olympus, Japan), precision balances (Mettler Toledo, Switzerland), an ultra-clean workbench (Shanghai Medical Device Company), adjustable volume pipettes (Eppendorf, USA), a vortex (Shanghai Medical Device Company), a thermostatic (temperature controlled) shaker (Taicang Huamei Biochemical Instrument Factory), an electrophoresis system (Tianneng Technology Co., Ltd., Shanghai), a thermal cycler (Biometra, USA), a laser scanning confocal microscope (Heidelberg, Germany), a s-520 scanning microscope (Hitachi, Japan), and a transmission electron microscope (Philips, Netherlands).
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