Droplet size and PDI were measured via dynamic light scattering analysis using a Zetasizer Nano-ZS model (Malvern Instruments Nordic AB, Skallestad, Norway). The measurement was performed at a temperature of 25°C and a scattering angle of 90° (Wang et al., 2019a (link)).
Zetasizer nanozs model
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer nanoZS model is a versatile laboratory instrument designed for the measurement of particle size, zeta potential, and molecular weight. It utilizes dynamic light scattering (DLS) and laser Doppler velocimetry (LDV) technologies to provide accurate and reliable data on the characteristics of nanoscale and submicron particles, molecules, and emulsions.
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Lab products found in correlation
Methylene blue, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Avantor (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Formaldehyde, by Avantor (1 mentions)
Methyl orange, by Merck Group (1 mentions)
Analyst 100 spectrophotometer, by PerkinElmer (1 mentions)
Spectraa 55 aas, by Agilent Technologies (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Dts1070, by Malvern Panalytical (1 mentions)
Spectrum one ftir spectrometer, by PerkinElmer (1 mentions)
8 protocols using zetasizer nanozs model
1
Dynamic Light Scattering Analysis of Droplet Size
2
Synthesis and Characterization of Polyethylenimine Nanoparticles
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Polyethylenimine (25000 Da) was purchased from BASF, phosphorous acid, formaldehyde, hydrochloric acid and sodium hydroxide were obtained from VWR and dyes (methylene blue, MB, and methyl orange, MO) from Sigma-Aldrich. Dialysis tubings were bought from Roth. Absorbance was measured by using a UV–visible Perkin-Elmer Analyst 100 spectrophotometer. The microwave used was a Prolabo Synthewave. A Varian SpectrAA 55 AAS was used to detect potential traces of iron in the supernatants after collecting nanoparticles. Zeta potential measurements were determined by DLS analysis using a Malvern Zetasizer nanoZS model.
3
Anti-VEGF Conjugation to LpAB-FeNP
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Anti-VEGF conjugation to LpAB-FeNP was performed using the EDC/NHS chemistry method [44 ]. Anti-VEGF was activated using 50.0 mM EDC and 12.5 mM NHS and then introduced to LpAB-FeNP solution. Anti-VEGF:EDC:NHS (1:2:2; v:v:v) was mixed in PBS and incubated at 250 rpm in an orbital shaker for 15 min. After that, the LpAB-FeNP/anti-VEGF conjugate was washed three times with PBS buffer using 50-kDa centrifugal filters at 4000 rpm. Dynamic light scattering technique (DLS, Malvern Zetasizer Nano ZS model) was used to measure the size distributions and zeta potential of LpAB-FeNP and LpAB-FeNP/anti-VEGF.
4
Evaluating Lipid-Based Formulation Characteristics
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The globule size of formulations was determined by a Zetasizer Nano ZS model (Malvern Instruments) and the zeta potential was determined by laser Doppler anemometry using the Malvern Zetasizer. All the SEDDS were diluted with TDW to an appropriate concentration before determining the zeta potential. The measurements were carried out in the fully automatic mode. Each sample was analyzed thrice43 (link).
5
Dynamic Light Scattering Analysis
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The DLS measurements have been performed on a Malvern Zetasizer nanoZS model equipped with a backscattering mode on the solutions containing the particles using the intensity profile. The sizes given in the article correspond to the Z average measurements.
6
Zeta Potential Measurement of Peptide-Bearing Nanoparticles
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The zeta potential values of the plain and peptide-bearing nanoparticles were also found with the Malvern Zetasizer Nano-ZS (Model ZEN3600) instrument by performing ELS. Each ELS sample was prepared by diluting 100 μl of the nanoparticle stock solution with 900 μl of 1X phosphate-buffered saline (PBS), as was done for the DLS samples. These dilutions were carefully pipetted into folded capillary zeta cells (Malvern part number DTS1070) to prevent bubble formation. These cells were loaded into the warmed Zetasizer one at a time by matching the arrow on the cell to the arrow on the instrument and closing the device's lid. The Malvern Zetasizer Nano-ZS software was used to take the zeta potential measurements. The program uses the absorption and refractive index of the silica core material ((Ri)Si = 1.459, A = 0.035) and the refractive index, dielectric constant, and viscosity of the PBS dispersant ((Ri)PBS = 1.330, K = 79, μ = 0.8882 cP) as well as its internal measurements to determine the zeta potential. The instrument reports three zeta potential measurements that correspond to the three trials the instrument automatically performs when run. The software displays the average zeta potential found over the 100 measurements the device takes during each trial.
7
Characterization of Clay Mineral Nanostructure
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Samples were analyzed using a PerkinElmer Spectrum One FT-IR Spectrometer. The unit was operated in a reflectance mode, with the scanning range between 550 cm -1 and 4000 cm -1 . Thermogravimetric analyses (TGA) of the samples were performed using a TGA Q50 model (TA according to Bragg's law. Crystallite domain size, corresponding to the (001) reflections, was calculated from the broadening of (001) XRD peak using the Scherrer equation:
Here, D001 is the mean crystallite domain size along the (001) direction; k is a dimensionless shape factor (k = 0.8λ)ν is the wavelength ( = 0.154 nm)ν is the line broading at full-width at halfmaximum (FWHM), in radiansν and is the diffraction angle. From the crystallite domain size and the d-spacing of the clay layers, the number of clay platelets per average stack (n), i.e. the average number of clay platelets stacked with high crystalline order, was calculated according to the equation 27 :
The zeta potential values of Na-MMT and of OMMT, dispersed in distilled water or in aqueous solutions of the hydrolyzed Remazol Black B dye (4 mg of the clay in 10 mL of water or the aqueous dye solution), were obtained using a Malvern Zetasizer-Nano ZS model (Malvern Instruments Ltd, UK).
Here, D001 is the mean crystallite domain size along the (001) direction; k is a dimensionless shape factor (k = 0.8λ)ν is the wavelength ( = 0.154 nm)ν is the line broading at full-width at halfmaximum (FWHM), in radiansν and is the diffraction angle. From the crystallite domain size and the d-spacing of the clay layers, the number of clay platelets per average stack (n), i.e. the average number of clay platelets stacked with high crystalline order, was calculated according to the equation 27 :
The zeta potential values of Na-MMT and of OMMT, dispersed in distilled water or in aqueous solutions of the hydrolyzed Remazol Black B dye (4 mg of the clay in 10 mL of water or the aqueous dye solution), were obtained using a Malvern Zetasizer-Nano ZS model (Malvern Instruments Ltd, UK).
8
Particle Size Analysis of Liposomes
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The blank and EF-loaded liposomes were subjected to measurement of particle size and PDI by a laser diffraction particle size analyzer (Zeta-sizer, Nano ZS model; Malvern Instruments UK, Milford, MA). Each sample was measured in triplicate and Z average (d. nm) and the poly-dispersity index (PDI) was determined.
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