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Ccr7 4b12

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CCR7 (4B12) is a mouse monoclonal antibody that recognizes the CCR7 chemokine receptor. CCR7 is a G-protein-coupled receptor that plays a role in the homing and recirculation of T cells and dendritic cells.

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Lab products found in correlation

Sa bv605, by BioLegend (2 mentions) Cd45 30 f11, by Cytek Biosciences (2 mentions) Sa 711, by BioLegend (2 mentions) Facscanto 2, by BD (2 mentions) Facsaria fusion, by BD (2 mentions) Cd103 2e7, by Thermo Fisher Scientific (2 mentions) Ab1225, by Merck Group (2 mentions) Nxt flow cytometer, by FlowJo (1 mentions)

3 protocols using ccr7 4b12

1

Quantitative Analysis of Immune Cell Markers

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Cells (107 cells/ml) were incubated with MNPs (150 µg Fe/ml) for 2 h in standard conditions, washed with PBS and stained with fluorochrome-conjugated Abs against cell surface markers. For Jurkat cells, the following anti-human antibodies were used: anti-CD62L (DREGC 56, Coulter), -CD11a (25.3.1, Immunotech), -CD45 (KC56 (T-200), Beckamn C.) and -CD44 (G44-26, Pharmingen). For murine primary T cells, the following anti-mouse antibodies from Biolegend were used: anti-CD44 (IM7), -CD62L (Mel-14), -CD11a (M17/4), -CCR7 (4B12), -CD4 (RMA-5) and -CD8 (53-6.7). CCR7 labelling was performed at 37 °C for 30 min with biotinylated primary antibody followed by secondary staining with PE-conjugated streptavidin. Data were acquired on a FC500 flow cytometer or in Attune Nxt flow cytometer and analysed with FlowJo software.
Sanz-Ortega L., Rojas J.M., Marcos A., Portilla Y., Stein J.V, & Barber D.F. (2019). T cells loaded with magnetic nanoparticles are retained in peripheral lymph nodes by the application of a magnetic field. Journal of Nanobiotechnology, 17, 14.
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2

Multicolor Flow Cytometry Panel

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Flow cytometry and cell sorting were completed on a FACS CantoII or FACS Aria Fusion instrument (BD) and analyzed using FlowJo analysis software (Tree Star). Staining was performed at 4ºC in the presence of Fc block (2.4G2) in magnetic-activated cell-sorting (MACS) buffer (PBS + .5% BSA + 2mM EDTA). The following antibodies were used; from BD Biosciences: CD117 (2B8), CD135 (A2F10.1), Ly6C (AL-21), MHCI (AF6–88.5), CD4 (RM4–5), CD8α (53–6.7), CD8β (53–5.8), CD11b (M1/70), B220 (RA3–6B2), CD64 (X54–5/7.1), CD19 (1D3), CD95 (Jo2), CD3 (145–2C11), CD45 (30-F11); from Tonbo Biosciences: MHCII (M5/114.15.2), CD44 (IM7), CD45.1 (A20), CD45.2 (104), CD11c (N418); from Biolegend: SA-BV605, SA-711, CCR7 (4B12), CD103 (2E7), XCR1 (ZET), CD115 (AFS98), Ter119 (Ter-119), Ly6G (1A8), TCRβ (H57–597), CD3 (145–2C11), CD8 (53–6.7), CD4 (RMA4–5), CD44 (IM7), CD40 (1C10), CD16/32 (93); from eBiosciences: TCRVα2 (B20.1), CD45.1 (A20), CD90.1 (HIS51), CD90.2 (53–2.1), F4/80 (BM8); from Invitrogen: CD172α (P84), CD45 (30F11); from Millipore/sigma: rabbit anti-ovalbumin (AB1225).
Ferris S.T., Durai V., Wu R., Theisen D.J., Ward J.P., Bern M.D., Davidson JT I.V., Bagadia P., Liu T., Briseño C.G., Li L., Gillanders W.E., Wu G.F., Yokoyama W.M., Murphy T.L., Schreiber R.D, & Murphy K.M. (2020). cDC1 prime and are licensed by CD4 T cells to induce anti-tumour immunity. Nature, 584(7822), 624-629.
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3

Multicolor Flow Cytometry Panel

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Flow cytometry and cell sorting were completed on a FACS CantoII or FACS Aria Fusion instrument (BD) and analyzed using FlowJo analysis software (Tree Star). Staining was performed at 4ºC in the presence of Fc block (2.4G2) in magnetic-activated cell-sorting (MACS) buffer (PBS + .5% BSA + 2mM EDTA). The following antibodies were used; from BD Biosciences: CD117 (2B8), CD135 (A2F10.1), Ly6C (AL-21), MHCI (AF6–88.5), CD4 (RM4–5), CD8α (53–6.7), CD8β (53–5.8), CD11b (M1/70), B220 (RA3–6B2), CD64 (X54–5/7.1), CD19 (1D3), CD95 (Jo2), CD3 (145–2C11), CD45 (30-F11); from Tonbo Biosciences: MHCII (M5/114.15.2), CD44 (IM7), CD45.1 (A20), CD45.2 (104), CD11c (N418); from Biolegend: SA-BV605, SA-711, CCR7 (4B12), CD103 (2E7), XCR1 (ZET), CD115 (AFS98), Ter119 (Ter-119), Ly6G (1A8), TCRβ (H57–597), CD3 (145–2C11), CD8 (53–6.7), CD4 (RMA4–5), CD44 (IM7), CD40 (1C10), CD16/32 (93); from eBiosciences: TCRVα2 (B20.1), CD45.1 (A20), CD90.1 (HIS51), CD90.2 (53–2.1), F4/80 (BM8); from Invitrogen: CD172α (P84), CD45 (30F11); from Millipore/sigma: rabbit anti-ovalbumin (AB1225).
Ferris S.T., Durai V., Wu R., Theisen D.J., Ward J.P., Bern M.D., Davidson JT I.V., Bagadia P., Liu T., Briseño C.G., Li L., Gillanders W.E., Wu G.F., Yokoyama W.M., Murphy T.L., Schreiber R.D, & Murphy K.M. (2020). cDC1 prime and are licensed by CD4 T cells to induce anti-tumour immunity. Nature, 584(7822), 624-629.
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