Fluorinert fc 3283

On days 0, 9, 11, and 15, mice were brought to a surgical plane of anesthesia with isoflurane then transcardially perfused with phosphate-buffered solution (PBS) followed by 4% paraformaldehyde (PFA) in 0.1 M PBS. Brains were extracted from the cranial vault and post-fixed in PFA. Imaged brains were placed in a custom-built holder immersed in Fluorinert (FC-3283, 3M, St. Paul, MN, USA) and imaged with a 4.7-T Agilent MRI system with a 3.5-cm diameter quadrature volume RF coil. Multi-slice, diffusion-weighted, spin echo images were used to acquire 10 non-diffusion weighted images (b = 0 s∙mm⁻²) and 75 diffusion-weighted images (25: b = 800 s∙mm⁻², 50: b = 2,000 s∙mm⁻²), using non-colinear diffusion-weighting directions. Other imaging parameters: TE/TR = 24.17/2000-ms, FOV = 30 × 30 mm², matrix = 192 × 192 reconstructed to 256 × 256 for an isotropic voxel size of 0.25-mm over two signal averages. All animals were used in subsequent analyses.