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Fluorinert fc 3283

Manufactured by 3M
Sourced in United States

Fluorinert (FC-3283) is a liquid fluorochemical product manufactured by 3M. It is a clear, colorless, and odorless liquid. Fluorinert (FC-3283) is designed for use as a heat transfer fluid in specialized laboratory equipment.

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2 protocols using fluorinert fc 3283

1

In Vivo Diffusion MRI of Mouse Brains

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On days 0, 9, 11, and 15, mice were brought to a surgical plane of anesthesia with isoflurane then transcardially perfused with phosphate-buffered solution (PBS) followed by 4% paraformaldehyde (PFA) in 0.1 M PBS. Brains were extracted from the cranial vault and post-fixed in PFA. Imaged brains were placed in a custom-built holder immersed in Fluorinert (FC-3283, 3M, St. Paul, MN, USA) and imaged with a 4.7-T Agilent MRI system with a 3.5-cm diameter quadrature volume RF coil. Multi-slice, diffusion-weighted, spin echo images were used to acquire 10 non-diffusion weighted images (b = 0 s∙mm−2) and 75 diffusion-weighted images (25: b = 800 s∙mm−2, 50: b = 2,000 s∙mm−2), using non-colinear diffusion-weighting directions. Other imaging parameters: TE/TR = 24.17/2000-ms, FOV = 30 × 30 mm2, matrix = 192 × 192 reconstructed to 256 × 256 for an isotropic voxel size of 0.25-mm over two signal averages. All animals were used in subsequent analyses.
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2

Structural Effects of Status Epilepticus in Rats

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Ex vivo imaging methods were used in male rats to examine structural effects of SE induced by IP KA at P15 (n=4), P25 (n=5), P35 (n=4), P70 (n=2) compared to normal controls (n=3). At ~P270 these rats were deeply anesthetized with pentobarbital 60 mg/kg IP and were transcardially perfused with fresh 4% paraformaldehyde (PFA). The fixed brains were removed and stored in 4% PFA for at least 1 month to standardize fixation, then were rinsed in 0.9% saline for 48 hours to minimize attenuating effects of fixative on the MRI signal. The brains were placed in a custom built holder and immersed in Fluorinert (FC-3283, 3M, St. Paul, MN). The sample holder was designed to hold six brains for high throughput imaging and positioning of each brain consistently within the spatial frame of the scanner.
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