Lc 20a pump

Manufactured by Shimadzu

Sourced in Japan

The Shimadzu LC-20A pumps are high-performance liquid chromatography (HPLC) pumps designed to deliver accurate and precise solvent flow for a variety of analytical applications. These pumps feature a compact design, easy-to-use interface, and reliable performance to support your analytical needs.

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LC-20A (357 citations) LC-20A module pumps (2 citations)

6 protocols using lc 20a pump

HPLC-MS/MS Analysis of Bioactive Compounds

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HPLC was performed on an instrument equipped with an SIL-82A autosampler, LC-20A pump, and SPD-20A detector (Shimadzu, Japan). The following chromatographic conditions were used: Kinetex C18 column (50 mm × 2.1 mm, 2.6 µm); mobile phase A, acetonitrile, and mobile phase B, 0.025% formic acid in water; column temperature, 35 °C; flow rate, 0.3 mL/min; and injection volume, 3 µL. The elution condition was as follows: 10–30% A at 0–5.5 min, 30–60% A at 5.5–9 min, and 60–90% A at 9–15 min.
MS analysis was performed by AB SCIEX Triple TOF 5600+ system (Framingham, MA, USA). Following MS conditions were used: ESI ion source, the scan range of 50–1200 Da, and scanning in positive and negative ion mode. The optimal MS parameter settings were as follows: ion spray voltage floating (ISVF), 4500 V; ion source gas 1(GS1), 55 psi; ion source gas 2(GS1), 55 psi; curtain gas (CUR), 35 psi; collision energy (CE), 45 V; temperature, 600 °C. The results were analyzed using Peak View software to extract information, such as fragment ions. The sample information was also compared with standard materials and literature for further identification.

He Y., Zheng H., Zhong L., Zhong N., Wen G., Wang L, & Zhang Y. (2021). Identification of Active Ingredients of Huangqi Guizhi Wuwu Decoction for Promoting Nerve Function Recovery After Ischemic Stroke Using HT22 Live-Cell-Based Affinity Chromatography Combined with HPLC-MS/MS. Drug Design, Development and Therapy, 15, 5165-5178.

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HPLC Analysis of Phytochemical Compounds

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The HPLC experiment was performed according to a previous study with some modifications [27 (link)]. The standard phytochemical compounds, including gallic acid, catechin, chlorogenic acid, caffeic acid, rutin, ferulic acid, myricetin, resveratrol, quercetin, kaempferol, and eugenol (Sigma-Aldrich, MO, USA) were used as standard compounds. The SAEE and standard compounds were dissolved in methanol (Merck, Darmstadt, Germany) at a concentration of 1 mg/mL each. Then, 100 μL of each sample was passed through a 0.20 μm particle size filter before injection into a C18 reversed-phase column (GL Sciences, Tokyo, Japan) using auto-injection (SIL-20A, Shimadzu, Tokyo, Japan). Acetonitrile (A) (Merck, Darmstadt, Germany) and 1% acetic acid (B) (RCI Labscan, BKK, Thailand) were used as the mobile phase at a flow rate of 1 mL/min using an LC-20A pump (Shimadzu, Tokyo, Japan). The mobile phase was run using gradient elution: 65% solvent B (0–15 min); 60% solvent B (15–28 min); 40% solvent B (28–40 min). The eluted compounds were detected using a UV detector (SPD-M20A, Shimadzu, Tokyo, Japan) at 280 nm. Peak areas of these compounds were compared with the standard compounds. The phytochemicals in the SAEE were calculated as µg/g dry weight.

Smanthong N., Tavichakorntrakool R., Tippayawat P., Lulitanond A., Pinlaor P., Daduang J., Sae-ung N., Chaveerach A., Phetcharaburanin J, & Boonsiri P. (2022). Anti-Proteus Activity, Anti-Struvite Crystal, and Phytochemical Analysis of Sida acuta Burm. F. Ethanolic Leaf Extract. Molecules, 27(3), 1092.

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Quantification of Anticancer Drugs by HPLC-MS/MS

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Drugs were quantified by a Shimadzu HPLC system coupled to a 3200 QTRAP mass spectrometer (Applied Biosystems, Grand Island, NY). The HPLC system consisted of two Shimadzu LC-20A pumps, a DGU-20A5 degasser, and a Shimadzu SIL-20AC HT autosampler. The mass spectrometer was equipped with an electrospray ionization (ESI) TurboIonSpray source. The system was operated with Analyst software, version 1.5.2 (ABSciex, Framingham, MA).
Chromatographic separation of drugs was achieved using a Synergi column (100 × 2.0 mm; 4- μm particle size) with an inline C8 guard column (4.0 × 2.0 mm) (Phenomenex, Torrance, CA). An ammonium acetate buffer/reagent alcohol gradient was used to separate components. Analytes were monitored using multiple-reaction monitoring for positive ions. The following ion transitions were monitored: gemcitabine, m/z 264.066→112.000; paclitaxel, m/z 854.266→286.200; a stable labeled isotope (C₈¹³CH₁₂ClF₂N¹⁵N₂O₄) (m/z 267.067→115.100) was used as an internal standard for gemcitabine; docetaxel (m/z 830.312→549.3) was used as an internal standard for paclitaxel.

Mu Q., Yu J., Griffin J.I., Wu Y., Zhu L., McConnachie L.A, & Ho R.J. (2020). Novel drug combination nanoparticles exhibit enhanced plasma exposure and dose-responsive effects on eliminating breast cancer lung metastasis. PLoS ONE, 15(3), e0228557.

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Phytochemical Fingerprinting of Medicinal Plants

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The analysis was performed by high-performance liquid chromatography (HPLC Prominence, LC-20A pumps, diode array detector DAD-UV SPD-M20A, Shimadzu, Shimadzu Corporation, Brazil) coupled to an Electrospray Ionization Mass Spectrometer (ESI-MS) with Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) from Bruker Corporation, MA, USA. Samples were injected onto a Shim-pack HR-ODS column (150 × 2.1 mm, 3 μm, Shimadzu Corporation, Brazil). Gradient elution was performed with 0.1% acetic acid/water (solvent A) and methanol (solvent B) at a constant flow rate of 0.2 ml/min. The elution gradient was 5–20% B over 30 min, 20% B isocratic 30–40 min, 20–95% B for 12 min, and re-equilibration of the column with 5% B until 64 min, using a flow rate of 200 μl/min. Spectra were recorded in negative and positive ionization mode between m/z 50 and 1200. Phenolic acids and flavonoids were also detected on silica gel 60 on HPTLC (High-performance thin-layer chromatography) plate 3 × 7 cm using ethyl acetate: dichloromethane: acetic acid: water (10:2.5:2:1, v/v) as the mobile phase. After drying, the plates were sprayed with the natural products reagent/polyethylene glycol (NP/PEG). The chromatograms were observed at 365 nm, and fluorescence bands were recorded and photographed.

Fernandes D.C., Martins B.P., Silva G.P., Fonseca E.N., Santos S.V., Velozo L.S., Gayer C.R., Sabino K.C, & Coelho M.G. (2021). Echinodorus macrophyllus fraction with a high level of flavonoid inhibits peripheral and central mechanisms of nociception. Journal of Traditional and Complementary Medicine, 12(2), 123-130.

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Quantification of Venetoclax and Zanubrutinib

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An extraction protocol was established to quantify concentrations of venetoclax and zanubrutinib in both nanoparticle-bound and free forms. Briefly, the drugs were solubilized by diluting the sample with ethyl acetate, which extracted them from either the DcNP complex, mouse plasma, or both. Following centrifugation, the supernatants were dried with nitrogen gas and then reconstituted in acetonitrile. Extracted drug solutions were then loaded onto a Shimadzu HPLC system coupled to a 3200 QTRAP mass spectrometer (Applied Biosystems, Grand Island, NY, USA). The HPLC system consisted of two Shimadzu LC-20A pumps, a DGU-20A5 degasser, and a Shimadzu SIL-20 AC HT autosampler. A Synergi Polar-RP column (100 × 2.0 mm) with a C₈ guard column (4.0 × 2.0 mm) (Phenomenex, Torrance, CA, USA) was used for separations. Mobile phase A used water with 20 mM ammonium acetate and B used acetonitrile. The separations were done at room temperature with a flow rate of 0.55 mL/min. The mass spectrometer was equipped with an electrospray ionization (ESI) TurboIonSpray source, and the system was operated using Analyst software, version 1.5.2 (ABSciex, Framingham, MA, USA). Drug concentrations in various samples were calculated with standard curves prepared from normal mouse plasma containing known drug concentrations.

Griffin J., Wu Y., Mu Q., Li X, & Ho R.J. (2023). Design and Characterization of a Novel Venetoclax-Zanubrutinib Nano-Combination for Enhancing Leukemic Cell Uptake and Long-Acting Plasma Exposure. Pharmaceutics, 15(3), 1016.

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Quantification of Curcumin in DPIs

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To quantify the curcumin content, a certain amount of curcumin-DPIs was dissolved in ethanol and mixed by ultrasonication for 10 min. The solution was diluted to a suitable concentration with ethanol and filtered through a 0.45-μm filter membrane. The subsequent filtrate was assayed by high-performance liquid chromatography (HPLC) to determine the concentration of curcumin. The HPLC system consisted of two LC-20A pumps and an SPD-20A UV/VIS detector (Shimadzu, Kyoto, Japan). The column used was Thermo C18 (250 × 4.6 mm). Acetonitrile/water (containing 0.05 % acetic acid glacial) (60:40, mL/mL) was used as the mobile phase with a flow rate of 1 mL/min. The wavelength was set at 428 nm.

Hu L., Kong D., Hu Q., Gao N, & Pang S. (2015). Evaluation of High-Performance Curcumin Nanocrystals for Pulmonary Drug Delivery Both In Vitro and In Vivo. Nanoscale Research Letters, 10, 381.

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