Edta coated microtainer tube

Manufactured by BD

Sourced in United States

EDTA-coated microtainer tubes are small containers used for collecting and storing blood samples. The tubes are pre-coated with EDTA (Ethylenediaminetetraacetic acid), an anticoagulant that helps prevent the blood from clotting during the collection and storage process.

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Lab products found in correlation

Procyte dx hematology analyzer, by IDEXX (2 mentions) Hematrue, by Heska (2 mentions) Hemavet 950 hematology system, by Drew Scientific (2 mentions) Gel separator, by BD (1 mentions) Anti cd41 antibody, by BD (1 mentions) Element ht5, by Heska (1 mentions) Wright giemsa, by Merck Group (1 mentions) Axiophot, by Zeiss (1 mentions) Hematrue veterinary hematology analyzer, by Heska (1 mentions) Microhematocrit capillary tube, by Thermo Fisher Scientific (1 mentions) Hemavet 950fs analyzer, by Drew Scientific (1 mentions) 2 methylbutane, by Thermo Fisher Scientific (1 mentions) Bm 698, by Boston BioProducts (1 mentions) Spectramax m3 microplate reader, by Molecular Devices (1 mentions) Konelab 20xt, by Thermo Fisher Scientific (1 mentions) Element ht5 veterinary hematology analyzer, by Heska (1 mentions) Microtainer serum separator tube, by BD (1 mentions)

Spelling variants of this product

EDTA tubes (290 citations) BD Microtainer (240 citations) Microtainer tubes (239 citations) EDTA-coated tubes (89 citations) EDTA Microtainer tubes (12 citations) Microtainer EDTA tubes (8 citations) EDTA microtainers (7 citations) EDTA-coated microtainers (5 citations)

19 protocols using edta coated microtainer tube

Comprehensive Blood Biomarker Analysis

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The blood was isolated and immediately collected in microcontainer tubes coated with clotting activators containing a gel separator (BD Biosciences, Plymouth, United Kingdom). Blood was allowed to clot for at least 30 min before serum separation by centrifugation at 3,000 rpm for 15 min. The biochemical analysis was performed using the automatic biochemical analyzer (Chemray 240) to measure albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine, urine protein, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (TC), and total cholesterol (TG) levels. For routine blood tests, the blood was isolated and immediately collected in EDTA-coated microtainer tubes (BD Biosciences). Blood cell compositions were analyzed using a ProCyte Dx Hematology Analyzer (IDEXX, Westbrook, ME, United States) following the manufacturer’s instructions.

He L., Lu H., Ji X., Chu J., Qin X., Chen M., Weinstein L.S., Gao J., Yang J., Zhang Q., Zhang C, & Zhang W. (2022). Stimulatory G-Protein α Subunit Modulates Endothelial Cell Permeability Through Regulation of Plasmalemma Vesicle-Associated Protein. Frontiers in Pharmacology, 13, 941064.

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Longitudinal Blood Profile Monitoring in Mice

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A total of 61 mice from two of the colonies (32 from the University of Nebraska Medical Center, and 29 from Sanford Research) were analyzed for complete blood counts (CBC’s) at 1, 3, 6, and 12 months-of-age. Submandibular blood collection was performed as previously described [23 (link)]. Briefly, mice were restrained, and a lancet was used to puncture the submandibular vein for blood collection into EDTA-coated microtainer tubes (BD, Franklin Lakes, NJ). All samples were analyzed on the day of collection. CBC’s were performed using a Scil Vet ABC Hematology Analyzer^® following the manufacturer’s suggested protocol with the mouse species card (Scil Vet, Gurnee, IL).

Timm D., Cain J.T., Geraets R.D., White K.A., Koh S.Y., Kielian T., Pearce D.A., Hastings M.L, & Weimer J.M. (2018). Searching for novel biomarkers using a mouse model of CLN3-Batten disease. PLoS ONE, 13(8), e0201470.

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Anti-CD41 Antibody-Induced Thrombocytopenia

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Mice were intraperitoneally injected with anti-CD41 antibody (BD Bioscience) either daily at 0.1 mg/kg body weight in PBS or on day 0 and 3 only each at 0.5 mg/kg body weight. Control mice were intraperitoneally injected with 400 μL PBS in parallel. Platelet counts were analyzed on a HemaTrue veterinary hematology analyzer (Heska Corporation) with 50 μL whole blood collected from the retro-orbital venous plexus into EDTA-coated microtainer tubes (BD Bioscience)

Yang J., Zhang Q., Li P., Dong T, & Wu M.X. (2016). Low-level light treatment ameliorates immune thrombocytopenia. Scientific Reports, 6, 38238.

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Quantification of E/Z-4-hydroxytamoxifen in Plasma

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Liquid chromatography with tandem mass spectrometry (LC/MS-MS) was performed on a Waters Xevo TQ-XS mass spectrometer system, as described previously.²⁰¹ (link) Standards were prepared using CD1 mouse plasma spiked with E/Z-4-hydroxytamoxifen-d5 internal standard (Cayman Chemicals, 34232). Peripheral blood was obtained from adult mice through retroorbital blood draws and stored in EDTA-coated Microtainer tubes (BD Biosciences, 365974). Blood samples were centrifuged for 2000g for 15 minutes at 4 °C to separate the plasma (supernatant), which was aliquoted and frozen at -20 °C before mass spectrometric analysis.

Fowler J.L., Zheng S.L., Nguyen A., Chen A., Xiong X., Chai T., Chen J.Y., Karigane D., Banuelos A.M., Niizuma K., Kayamori K., Nishimura T., Cromer M.K., Gonzalez-Perez D., Mason C., Liu D.D., Yilmaz L., Miquerol L., Porteus M.H., Luca V.C., Majeti R., Nakauchi H., Red-Horse K., Weissman I.L., Ang L.T, & Loh K.M. (2024). Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells. Developmental Cell, 59(9), 1110-1131.e22.

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Collecting and Analyzing Mouse Blood

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Peripheral blood was collected via tail vein in EDTA-coated Microtainer tubes (BD) and kept on ice. CBCs were analyzed on an Element HT5 (HESKA) instrument.

Goldstein J.M., Valido A., Lewandowski J.P., Walker R.G., Mills M.J., Messemer K.A., Besseling P., Lee K.H., Wattrus S.J., Cho M., Lee R.T, & Wagers A.J. (2019). Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Scientific Reports, 9, 18613.

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Hematological and Histological Analysis

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Blood smears and complete blood cell counts (CBC) were evaluated for pathologies. For CBC, blood was collected via tail vein into EDTA-coated microtainer tubes (BD Bioscience) and analyzed on a HemaTrue veterinary hematology analyzer (Heska Corporation). Serum AST was measured using a DRI-CHEM analyzer (Heska). Peripheral blood smears were stained with Wright-Giemsa (Sigma-Aldrich). Formalin fixed tissues from lung, spleen and kidney were embedded, cut and stained with hematoxylin and eosin (H&E) for histological analysis. Microscopic analysis was conducted with a Zeiss Axiophot and images were captured using Picture Frame 2.3 software.

Ramsey H, & Wu M.X. (2014). Mitochondrial anti-oxidant protects IEX-1 deficient mice from organ damage during endotoxemia. International immunopharmacology, 23(2), 658-663.

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Blood Collection for GBM Analysis

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Blood from GL26 GBM bearing mice was taken from the submandibular vein and transferred to EDTA coated microtainer tubes (BD Biosciences) or serum separation tubes (Biotang). Samples in the serum separation tubes were left at room temperature for 20 min to allow for blood coagulation before centrifugation at 2000 rpm (400 × g). Complete blood cell counts and serum chemistry for each sample were determined by in vivo animal core at the University of Michigan.

Gregory J.V., Kadiyala P., Doherty R., Cadena M., Habeel S., Ruoslahti E., Lowenstein P.R., Castro M.G, & Lahann J. (2020). Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy. Nature Communications, 11, 5687.

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Peripheral Blood Collection and Analysis

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Peripheral blood was obtained by retro-orbital bleeding using heparinized micro-hematocrit capillary tubes (Fisherbrand), and collected in EDTA-coated Microtainer tubes (BD). Complete blood counts were assessed by VetScan HM5 instrumentation. Unpaired two-tailed Student’s t-test was used for statistical analysis. *p<0.05, **p<0.01, ***p<0.001.

Yu W., Goncalves K.A., Kishikawa H., Li S., Sun G., Yang H., Vanli N., Wu Y., Jiang Y., Hu M.G., Friedel R.H, & Hu G.F. (2017). Plexin-B2 mediates physiologic and pathologic functions of angiogenin. Cell, 171(4), 849-864.e25.

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Comprehensive Blood Cell Analysis

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Peripheral blood from the femoral vein was collected in EDTA-coated Microtainer Tubes (BD, Heidelberg, Germany). Blood counts were measured using the ProCyte Dx Hematology Analyzer (IDEXX Laboratories, Westbrook, Maine) and the Sysmex TX2000 blood analyzer (Sysmex, Kobe, Japan). Blood smears were stained with Wright-Giemsa according to standard protocols. Differential cell count analysis was obtained by the CellaVision DM 1200 (CellaVision AB, Lund, Sweden).

Schmidt K., Zhang Q., Tasdogan A., Petzold A., Dahl A., Arneth B.M., Slany R., Fehling H.J., Kranz A., Stewart A.F, & Anastassiadis K. (2018). The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice. eLife, 7, e27157.

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Blood Collection and Platelet Count

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Blood was collected via tail vein into EDTA-coated microtainer tubes (BD Bioscience) and platelet counts were attained on a HemaTrue veterinary hematology analyzer (Heska Corporation; Loveland, CO).

Ramsey H., Zhang Q, & Wu M.X. (2014). Mitoquinone restores platelet production in irradiation-induced thrombocytopenia. Platelets, 26(5), 459-466.

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