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Tnf α

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TNF-α is a recombinant human tumor necrosis factor alpha (TNF-α) protein. TNF-α is a cytokine involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types as well.

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Lab products found in correlation

Ifn γ, by Mesoscale (3 mentions) Interleukin 6 (il 6), by Mesoscale (3 mentions) Il 1β, by Mesoscale (2 mentions) Interleukin 2 (il 2), by Mesoscale (2 mentions) Mcp 1, by Mesoscale (2 mentions) Il 12p70, by Mesoscale (2 mentions) Gm csf, by Mesoscale (1 mentions) Complex 2, by Abcam (1 mentions) Complex 1, by Abcam (1 mentions) Kc gro, by Mesoscale (1 mentions) Il 10, by Mesoscale (1 mentions)

Close spelling variants of this product

U-PLEX combo kit against TNF-α

9 protocols using tnf α

1

Multiplex Cytokine Profiling

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We performed multiplex assays to measure IL-1β, IL-2, IL-6, TNFα, and IFN-γ purchased from Meso Scale Discovery. All experimental operations were in accordance with standard protocols. R2s of the standard curves for each plate were greater than 0.999.
Bremner J.D., Gurel N.Z., Jiao Y., Wittbrodt M.T., Levantsevych O.M., Huang M., Jung H., Shandhi M.H., Beckwith J., Herring I., Rapaport M.H., Murrah N., Driggers E., Ko Y.A., Alkhalaf M.L., Soudan M., Song J., Ku B.S., Shallenberger L., Hankus A.N., Nye J.A., Park J., Vaccarino V., Shah A.J., Inan O.T, & Pearce B.D. (2020). Transcutaneous vagal nerve stimulation blocks stress-induced activation of Interleukin-6 and interferon-γ in posttraumatic stress disorder: A double-blind, randomized, sham-controlled trial. Brain, Behavior, & Immunity - Health, 9, 100138.
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2

Multiplex Assay for Biomarker CSF Levels

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Meso Scale Discovery (MSD) multiplex assay was used to determine CSF levels for TNF-α (Cat. No. K15049G, MSD, Rockville, MD, USA).
Contreras J.A., Aslanyan V., Sweeney M.D., Sanders L.M., Sagare A.P., Zlokovic B.V., Toga A.W., Han S.D., Morris J.C., Fagan A., Massoumzadeh P., Benzinger T.L, & Pa J. (2019). Functional connectivity among brain regions affected in Alzheimer’s disease is associated with CSF TNF-α in APOE4 carriers. Neurobiology of aging, 86, 112-122.
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3

Multiplex Inflammatory Biomarker Profiling

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A broad panel of inflammatory biomarkers was measured by a multiplex high-sensitivity assay, including interleukin-1β (IL-1β), IL-6, IL-8, IL-10, IL-12, interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) (Meso Scale Discovery, Maryland, USA). Serum C-reactive protein (CRP) was measured by immunoturbidometry (Cobas, Roche Diagnostic, Mannheim, Germany).
Habbab K.M., D’Aiuto F., Habbab M.A, & Porter S.R. (2019). Molecular markers relevant to myocardial injury following dental extraction in patients with and without coronary artery disease. BDJ Open, 5, 9.
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4

Measuring Neuroinflammatory Markers in ART-Naïve Individuals

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We selected monocyte and macrophage activation markers, as well as specific inflammatory markers that have previously been associated with NCI in ART-naïve individuals35 (link)36 (link)37 (link). Levels of selected monocyte activation and inflammatory markers were measured in CSF and blood plasma by enzyme-linked immunosorbent assay (ELISA): sCD163 (Trillium Diagnostics, Brewer, ME, USA); or by electrochemiluminescence multiplex assay: MCP-1, IL-8, IL-6 and TNF-α, (Meso Scale Diagnostics, Rockville, MD, USA), according to the manufacturer’s procedures.
Oliveira M.F., Murrel B., Pérez-Santiago J., Vargas M., Ellis R.J., Letendre S., Grant I., Smith D.M., Woods S.P, & Gianella S. (2015). Circulating HIV DNA Correlates With Neurocognitive Impairment in Older HIV-infected Adults on Suppressive ART. Scientific Reports, 5, 17094.
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5

Multiplex Cytokine and Chemokine Analysis

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U-Plex 10 Assay Kits for analysis of IL-1b, IL-6, IL-10, IL-12p70, TNF-α, VEGFa, IP10, MCP1, TARC, and MDC were purchased from Meso Scale Diagnostics (MSD, USA). The assay was performed as described by the manufacturer. ELISA kits to measure MMP1 (DY901B), MIP1-a (DY270), MIP1-b (DY271), and IL-2 (DY202) were obtained from R&D Systems. The supernatants were tested according to the manufacturer's instructions in 96-well half area plates using 50 μL sample/test.
Tosiek M.J., Groesser K., Pekcec A., Zwirek M., Murugesan G, & Borges E. (2022). Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response. Journal of Immunology Research, 2022, 9926305.
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6

Multiplex Serum Biomarker Analysis

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Paired pre- and end of treatment (week 24) cryopreserved serum samples were tested by enzyme-linked immunosorbent assays (ELISA) in multiplex for tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, IL-2, IL-8, IL-10, IL-12p70, interferon gamma (IFNγ), and GM-CSF (MesoScale Discovery, Gaithersburg, MD). IL-7, osteopontin and tissue inhibitor of metalloproteinases-1 (TIMP1) were quantitated using single-plex assays, while TGF-β isoforms 1, 2 and 3 were quantitated using a triplex assay (MesoScale Discovery). Cleaved cytokeratin-18 levels were quantitated by ELISA (M30 Apoptosense, Peviva, West Chester, OH).
Meissner E.G., McLaughlin M., Matthews L., Gharib A.M., Wood B.J., Levy E., Sinkus R., Virtaneva K., Sturdevant D., Martens C., Porcella S.F., Goodman Z.D., Kanwar B., Myers R., Subramanian M., Hadigan C., Masur H., Kleiner D.E., Heller T., Kottilil S., Kovacs J.A, & Morse C.G. (2016). Simtuzumab treatment of advanced liver fibrosis in HIV and HCV-infected adults: Results of a 6-month open-label safety trial. Liver international : official journal of the International Association for the Study of the Liver, 36(12), 1783-1792.
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7

Multiplex Assay for Biomarker CSF Levels

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Meso Scale Discovery (MSD) multiplex assay was used to determine CSF levels for TNF-α (Cat. No. K15049G, MSD, Rockville, MD, USA).
Contreras J.A., Aslanyan V., Sweeney M.D., Sanders L.M., Sagare A.P., Zlokovic B.V., Toga A.W., Han S.D., Morris J.C., Fagan A., Massoumzadeh P., Benzinger T.L, & Pa J. (2019). Functional connectivity among brain regions affected in Alzheimer’s disease is associated with CSF TNF-α in APOE4 carriers. Neurobiology of aging, 86, 112-122.
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8

ELISA Quantification of Complex I, II, and TNFα

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Complex I, Complex II (Abcam, Cambridge, MA, USA), and TNFα (Meso Scale Discovery, Rockville, MD, USA) assays were measured by ELISA. All samples were analyzed in duplicate and run in a single assay with intra-assay and percent coefficients of variability of less than 10% for all assays.
Thorwald M.A., Godoy-Lugo J.A., Rodriguez R., Stanhope K.L., Graham J.L., Havel P.J., Forman H.J, & Ortiz R.M. (2022). Cardiac NF-κB Acetylation Increases While Nrf2-Related Gene Expression and Mitochondrial Activity Are Impaired during the Progression of Diabetes in UCD-T2DM Rats. Antioxidants, 11(5), 927.
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9

BALF Cytokine Profiling via MSD

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The BALF supernatant samples were analysed using MSD pro-inflammatory panel V-Plex including the following analytes: IFN-γ, IL-10, IL-12p70, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO and TNF-α (Meso Scale Diagnostics, Rockville, MD). The analysis was performed according to the manufacturer’s instruction.
Wronski S., Dannenmaier J., Schild S., Macke O., Müller L., Burmeister Y., Seilheimer B, & Müller M. (2018). Engystol reduces onset of experimental respiratory syncytial virus-induced respiratory inflammation in mice by modulating macrophage phagocytic capacity. PLoS ONE, 13(4), e0195822.
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