Spectra por

CAL-B (Novozyme 435), which is a lipase derived from Candida Antarctica type B and immobilized on acrylic resin, was purchased from Sigma Aldrich, St. Louis, MO, USA. Prior to its use, it was first vacuum-dried over Phosphorous pentoxide for 24 h. Sorbitol (98%) and divinyl adipate (96%) were purchased from Sigma Aldrich (Steinheim, Germany) and TCI GmbH (Eschborn, Germany), respectively. Phosphorous pentoxide (≥99%), 4-(dimethylamino)pyridine (DMAP), anhydrous N,N-dimethylformamide (DMF, 99.8%), anhydrous tetrahydrofuran (THF, 99.9%), acetonitrile (anhydrous, 99.8%), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), dialysis membranes with 1000 g·mol⁻¹ molecular weight cut off (MWCO), and 10,000 g·mol⁻¹ MWCO (Spectra/Por^®, made from regenerated cellulose) were purchased from Carl Roth, Karlsruhe, Germany. α,ω-bis-hydroxy poly(ethylene glycol)_n (where n = 9, 23, and 45) and α- methoxy,ω-hydroxy poly(ethylene glycol)_n (where n = 12) (mPEG 550) were purchased from Alfa Aesar, Kandel, Germany. DY-781 Amine (molecular weight: 781 g·mol⁻¹) and DY-784 NHS-ester (molecular weight: 1188 g·mol⁻¹) were purchased from Dyomics GmbH (Jena, Germany), while BSA-TMR was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA).

Novozyme 435, Lipase derived from Candida Antarctica type B (CAL-B) and immobilized on acrylic resin, was purchased from Sigma Aldrich, St. Louis, MO, USA. It was vacuum dried over Phosphorous pentoxide for 24 h prior to use. Sorbitol (98%) and divinyl adipate were purchased from Sigma Aldrich (Steinheim, Germany) and TCI GmbH (Eschborn, Germany), respectively. Phosphorous pentoxide (≥99%), 4-(dimethylamino)pyridine (DMAP), anhydrous N,N-dimethylformamide (DMF, 99.8%), anhydrous tetrahydrofuran (THF, 99.9%), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl), dialysis membranes with 1000 g·mol⁻¹ molar mass cut off (MWCO) and 10,000 g·mol⁻¹ MWCO (Spectra/Por^®, made from regenerated cellulose) were purchased from Carl Roth, Karlsruhe, Germany. Deuterated chloroform (CDCl₃) and deuterated dimethyl sulfoxide (DMSO-d₆) were purchased from Armar (Europa) GmbH (Leipzig, Germany). α,ω-bis-hydroxy poly(ethylene glycol)_n (OH-PEG_n-OH, with n = 9, 23, and 45) and α-methoxy,ω-hydroxy poly(ethylene glycol)₁₂ (mPEG₁₂-OH) were purchased from Alfa Aesar (Kandel, Germany).

The poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (M_n = 250,000 g/mol; dispersity index M_w/M_n = 2.5; the 4HB unit content 8.8 mol %) was purchased from Tianjin Green Bio-Science (Tianjin, China); the high molecular weight poly-γ-glutamic acid (M_n = 150,000 g/mol; dispersity index M_w/M_n = 2.05) and ultra-low molecular weight poly-γ-glutamic acid (M_n = 2000 g/mol; dispersity index M_w/M_n = 1.43) were purchased from Shandong Freda Biotechnology Co., Ltd. (Shandong, China). Tetradecyltrimethylammonium bromide, 4-toluenesulfonic acid monohydrate, and [R,S]-β-butyrolactone were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Dimethyl sulfoxide (DMSO) and N,N-dimethylformamide (DMF) were purchased from POCH SA (Gliwice, Poland). Dialysis membrane Spectra/Por (MWCO 1000) was purchased from Carl Roth (Karlsruhe, Germany).