Anti cyclin d1

Ten patients were subjected to conventional H&E preparation and immunohistochemical staining. Microscopy was performed by two pathology doctors (WJ and JNC) according to the 2008 WHO classification of lymphoma neoplasms, and then two senior doctors (QL and CKS) rechecked the microscopy and made the final diagnosis. Antibodies CK, LCA, CD3, CD45RO, CD20, CD79a, CD5, Cyclin D1, CD10, MUM1, Bcl2, Bcl6, CD30, and Ki-67 were obtained from Abcam USA and DAKO Denmark. The processes were as follows: immunohistochemical staining for 4-μm tissues were performed with anti-CK (1:100), anti-LCA (1:400), anti-CD3 (1:100), anti-CD45RO (1:500), anti-CD20 (1:250), anti-CD79a (1:100), anti-CD5 (1:100), anti-Cyclin D1 (RTU), anti-CD10 (1:50), anti-MUM1 (1:50), anti-Bcl2 (1:100), anti-Bcl6 (1:100), anti-CD30 (1:40), and anti-Ki-67 (1:100) and were detected using the DAKO En-Vision System (Dako Diagnostics, Zug, Switzerland) according to the manufacturer’s instructions.

Anti-p53, anti-p21^waf1, anti-cyclin D1, and anti-bcl2 antibodies were purchased from Dako (Copenhagen, Denmark). Anti-HNF-1β, anti-GSK-3β, anti-Rb, anti-p27^kip1, anti-XIAP, anti-bax, and anti-integrin β1 antibodies were obtained from BD Biosciences (San Jose, CA, USA). Anti-ARID1A, anti-cyclin B1, and anti-MDM2 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-Snail, anti-Akt, anti-phospho(p) Akt Serine473, anti-pGSK-3β Serine9, anti-pRb Serine807/811, anti-cleaved caspase-3, and anti-integrin β3 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-fibronectin (FN), anti-E-cadherin, and anti-β-actin antibodies were obtained from Abcam (Cambridge, MA, USA), Takara (Shiga, Japan), and Sigma-Aldrich Chemicals (St. Louis, MO, USA), respectively. Anti-cyclin A2 and anti-integrin β2 antibodies were from Novocastra (Newcastle, UK) and Merck KGaA (Darmstadt, Germany), respectively. FN (catalog number #F2006) and cisplatin (CDDP: #479306) were purchased from Sigma-Aldrich Chemicals.

Anti-Nodal and anti-β-actin antibodies as well as CDDP, doxorubicin (Dox), MG132, and lithium chloride (LiCl) were purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Anti-X-linked inhibitor of apoptosis (XIAP), anti-Bax, anti-Glycogen Synthase Kinase (GSK)-3β, and anti-p27^Kip1 antibodies were bought from BD Biosciences (San Jose, CA, USA). Anti-p21^waf1, anti-Cyclin D1, anti-Bcl2, and anti-Ki-67 antibodies were purchased from Dako (Copenhagen, Denmark). Anti-cleaved caspase-3 and anti-phospho(p) GSK-3β at Ser9 (pGSK-3β) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-Smad2 and anti-pSmad2 at Serine 255 (pSmad2) antibodies were from Abcam (Cambridge, MA, USA). Anti-Cyclin A2 antibody was from Novocastra (Newcastle, UK). Recombinant transforming growth factor (TGF)-β1 was purchased from R&D Systems (Minneapolis, MN, USA).

Anti-ALK, Anti-Rb phospho-Ser807/811 (pRb), anti-CD56, anti-synaptophysin (Syn), and anti-vimentin, anti-Slug, anti-cleaved caspase-3, and anti-cleaved poly (ADP-ribose) polymerase 1 (PARP1) antibodies were purchased from Cell Signaling (Danvers, MA, USA). Anti-Sox7, anti-ZEB1, and anti-β-actin antibodies were obtained from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Anti-Snail, anti-Nestin, and anti-Sox2 antibodies were from Abcam (Cambridge, MA, USA). Anti-Rb, anti-p27^kip1, anti-N-cadherin, anti-BAX, anti-X-linked inhibitor of apoptosis (XIAP), and anti-aldehyde dehydrogenase (ALDH)1 antibodies were from BD Biosciences (San Jose, CA, USA). Anti-Ki-67, anti-p21^waf1, anti-cyclin D1, anti-p53, anti-BCL2, and anti-CD44s antibodies were from Dako (Glostrup, Denmark). Anti-cyclin A2, anti-cyclin B1, and anti-CD133 antibodies were from Novocastra (Newcastle, UK), Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Miltenyi Biotechnology (Bergish Gladbach, Germany), respectively. Adriamycin (ADR: Catalog No. #D1515) was purchased from Sigma-Aldrich Chemicals.

Anti-LEFTY, anti-Smad2, and anti-phospho(p)-Smad2 at serine 255 (pSmad2) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-p27^Kip1 and anti-glycogen synthase kinase (GSK)-3β antibodies were bought from BD Biosciences (San Jose, CA, USA). Anti-p21^waf1, anti-cyclin D1, and anti-Ki-67 antibodies were purchased from Dako (Copenhagen, Denmark). Anti-cyclin A2, anti-estrogen receptor α (ERα), and anti-progesterone receptor (PR) antibodies were from Novocastra (Newcastle, UK). Anti-pGSK-3β at Ser9 (pGSK-3β), anti-Akt, anti-pAkt at serine 473 (pAkt), and anti-ubiquitin antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin antibody, nocodazole, 17β-estradiol (E2), medroxyprogesterone 17-acetate (MPA), MG132, and lithium chloride (LiCl) were purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Rapamycin and aphidicolin were obtained from Calbiochem (Cambridge, MA, USA). Recombinant TGF-β1 was purchased from R&D Systems (Minneapolis, MN, USA).

Anti-β-catenin and anti-p27^kip1 antibodies were purchased from BD Biosciences (San Jose, CA, USA). Anti-Sox4, anti-Sox6, anti-Sox7, anti-Sox9, anti-Sox11, and β-actin antibodies were obtained from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Anti-Snail and anti-Slug antibodies were from Cell Signaling (Danvers, MA, USA). Anti-p21^waf1, anti-cyclin D1, and anti-CD44s antibodies were purchased from Dako (Copenhagen, Denmark). Anti-Sox2 and anti-cyclin A antibodies were from Abcam (Cambridge, MA, USA) and Novocastra (Newcastle, UK), respectively. Anti-HA and anti-E-cadherin antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and Takara (Shiga, Japan) respectively. Anti-CD133 antibody was from Miltenyi Biotechnology (Bergisch Gladbach, Germany).
STK2, which is a serum-free culture medium for mesenchymal stem cells, [22 (link)] was obtained from DS Pharma Biomedical (Osaka, Japan).

Anti-FLAG M2 and anti-β-actin antibodies were purchased from Sigma-Aldrich Chemicals. Anti-p21^waf1, anti-cyclin D1, anti-p53, anti-BCL2, and anti-Ki-67 antibodies were obtained from Dako (Glostrup, Denmark). Anti-p27^kip1 and anti-BAX antibodies were from BD Biosciences (San Jose, CA, USA). Anti-MDM2 and anti-S100A1 were from Abcam (Cambridge, MA, USA). Anti-cleaved caspase-3 and anti-cleaved poly (ADP-ribose) polymerase 1 (PARP1)(Asp214)(D64E10) were from Cell Signaling Technology (Danvers, MA, USA). Anti-cyclin B1 and anti-cyclin A2 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Novocastra (Newcastle, UK), respectively.
Rapamycin, aphidicolin, and nocodazole for synchronization of cells at G1, early S, and G2/M phases, respectively, were obtained from Calbiochem (Cambridge, MA, USA). Adriamycin (ADR) and Nutlin-3A were from Sigma-Aldrich Chemicals.