Sodium chloride (NaCl), calcium chloride (CaCl2) potassium phosphate monobasic (KH2PO4), potassium phosphate dibasic (K2HPO4), sodium hydroxide (NaOH), sodium phosphate dibasic (Na2HPO4), sodium hypochlorite (NaClO) solution 10% w/v, dimethyl sulfoxide (DMSO), acetonitrile (ACN), hydrochloric acid (HCl), methanol (MeOH), trifluoroacetic acid (TFA) and tert-butyl hydroperoxide (tBuOOH) were purchased from Panreac (Barcelona, Spain). Agar, cholesterol, sodium ampicillin, nystatin, bacto-yeast, 5-fluoro-2′-deoxyuridine (FUdR), phosphate buffered saline and 2’,7’-dichlorofluorescein diacetate (DFCH-DA) were purchased from Sigma-Aldrich (Madrid, Spain). Peptone enzymatic digest from soybean, bacto-tryptone and trichloroacetic acid (TCA) were provided by Fluka (Madrid, Spain). Magnesium sulphate, tween 20 (poly-oxy-ethyl-ensorbitan-monolaurate) and acetic acid were acquired from Merck (Darmstadt, Germany). Water (H2O) was purified by using a Milli-Q Plus185 system from Millipore (Milford, MA, USA). Reversed-phase C18 resin (12–20 µm) was purchased from Sigma-Aldrich.
Sodium phosphate dibasic
Manufactured by ITW Reagents
Sourced in Spain
Sodium phosphate dibasic is a chemical compound with the formula Na2HPO4. It is a white, crystalline solid that is soluble in water. Sodium phosphate dibasic is commonly used as a buffer in various laboratory applications.
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Lab products found in correlation
Sodium hydroxide (naoh), by ITW Reagents (2 mentions)
Magnesium sulphate, by Merck Group (1 mentions)
Sodium chloride (nacl), by ITW Reagents (1 mentions)
Nystatin, by Merck Group (1 mentions)
Ampicillin sodium, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
5 fluoro 2 deoxyuridine, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Potassium phosphate monobasic, by ITW Reagents (1 mentions)
Sodium hypochlorite, by ITW Reagents (1 mentions)
Bacto yeast, by Merck Group (1 mentions)
Milli q plus185 system, by Merck Group (1 mentions)
Lb agar, by Merck Group (1 mentions)
Magnesium sulfate heptahydrate, by Merck Group (1 mentions)
Tryptone, by Merck Group (1 mentions)
Cobalt 2 chloride hexahydrate, by Merck Group (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Dnase, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
4 protocols using sodium phosphate dibasic
1
Analytical Reagents and Solutions Protocol
2
Preparation of Ultrapure Reagents
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Ultrapure reagent-grade water was obtained from a Mili-Q system (Millipore/Waters). Carbenicillin disodium salt, calcium chloride dihydrate, magnesium sulfate heptahydrate, lysozyme, cobalt(II) chloride hexahydrate, dithiothreitol (DTT), SAM chloride salt, DNase, epinephrine (bitartrate salt), disodium ethylenediamine tetraacetic acid (EDTA), sodium octyl sulfate (OSA), bovine serum albumin (BSA), LB-Agar, IPTG, tryptone, glycerol and propidium iodide (PI) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Potassium chloride, sodium chloride, boric acid were supplied by Fluka (Buchs, Switzerland). Sodium phosphate dibasic and potassium dihydrogen phosphate monobasic were obtained from Panreac (Barcelona, Spain). Bis-(1,3-dibutylbarbituric acid)trimethine oxonol (BOX) was obtained from Molecular Probes®, Invitrogen, part of Life Technologies (Carlsbad, CA, USA). All other chemicals were of analytical grade and used without further purification.
3
Synthesis of Silver Nanoparticles
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Acetone was obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium ascorbate was obtained from Fluka. Dibasic sodium phosphate, monobasic sodium phosphate and sodium nitrite were obtained from Panreac. Silver conductive paint was purchased from RS Components. All reagents were of analytical grade. Solutions were prepared with deionized water (≥18 MΩ cm) from a Millipore Milli-Q purification system.
4
Characterization of β-D-Galactosidase Enzymatic Activity
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β-D-Galactosidase, with an enzymatic activity of 73654 acid lactase units per gram (ALU/g), was donated by Laboratorios Salvat S.A. (Spain). α-Cyclodextrin (α-CD), from Wacker Chemie AG (water content approximately 8%), was used as received. The poloxamine Tetronic 90R4 (Sigma-Aldrich) was a yellowish, viscous liquid (3870 cP at 25 °C) with an average molecular weight M90R4 ≈ 7200 g/mol (as given by the manufacturer). The chemical composition was determined by 1H NMR: PO16EO18 per arm (Figure 6 ).
Dissolution media were prepared using type I deionized water (Millipore Elix 3, minimum resistivity at 25 °C, 18 MOhm, electrical conductivity <0.1 µS/cm), hydrochloric acid (37%, Panreac, Spain), dibasic sodium phosphate and monobasic potassium phosphate (99% purity, Panreac, Spain).
In order to determine the activity of the β-D-galactosidase (lactase), 2-nitrophenyl-galactopyranoside (ONPG) (Sigma-Aldrich, ≥98% purity, 301.3 g/mol) was used as a substrate to simulate the action of the enzyme on lactose and its dissociation. The ONPG substrate requires a slightly acidic medium for enzyme lactase activity, therefore a buffer (pH 4.5) was used according to USP. Sodium hydroxide, acetic acid, and sodium carbonate (Panreac, Spain) were used as reagents.
Dissolution media were prepared using type I deionized water (Millipore Elix 3, minimum resistivity at 25 °C, 18 MOhm, electrical conductivity <0.1 µS/cm), hydrochloric acid (37%, Panreac, Spain), dibasic sodium phosphate and monobasic potassium phosphate (99% purity, Panreac, Spain).
In order to determine the activity of the β-D-galactosidase (lactase), 2-nitrophenyl-galactopyranoside (ONPG) (Sigma-Aldrich, ≥98% purity, 301.3 g/mol) was used as a substrate to simulate the action of the enzyme on lactose and its dissociation. The ONPG substrate requires a slightly acidic medium for enzyme lactase activity, therefore a buffer (pH 4.5) was used according to USP. Sodium hydroxide, acetic acid, and sodium carbonate (Panreac, Spain) were used as reagents.
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