To determine the invasiveness of pCMV/HCT-116 or AKT/HCT-116 cells, invasion assays were performed and evaluated by employing Boyden chambers equipped with polyethylene terephthalate membranes with 8-μm pores (BD Biosciences, San Jose, CA, USA). The cells were cultured in complete medium for 24 h prior to detachment with trypsin EDTA. Subsequently, 5 × 104 cells per chamber were re-suspended in culture medium and layered on the Matrigel, and after an additional 24 h, the invaded cells were counted using an AMG EVOS digital inverted microscope (Life Technologies, Carlsbad, CA, USA) as described earlier [24 (link)].
Amg evos digital inverted microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The AMG EVOS digital inverted microscope is a versatile imaging system designed for a variety of cell culture and microscopy applications. It features a high-resolution digital camera, advanced optics, and user-friendly software for capturing and analyzing images.
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Lab products found in correlation
3 protocols using amg evos digital inverted microscope
1
Assessing Cell Invasion Potential
2
Evaluating Invasive Behavior in Cell Lines
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The invasive behavior of HCT 116, SW-620, pCMV/HCT 116, and AKT/HCT 116 was evaluated in Boyden chambers equipped with polyethylene terephthalate membranes with 8 μm pores. Approximately 5 × 104 cells per chamber were resuspended in culture medium with vehicle (DMSO) or VJ and layered on the Matrigel (Corning BioCoat™ Matrigel Invasion Chamber, Bedford, MA, USA). After an additional 24 h, the invading cells were counted using an AMG EVOS digital inverted microscope (Life Technologies, Carlsbad, CA, USA) as previously described [35].
3
Evaluating Invasiveness of Lung Cancer Cells
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To determine the invasiveness of H460 and A549 cells, we performed invasion assays and evaluated them by employing Boyden chambers equipped with polyethylene terephthalate membranes with 8-μm pores (Corning, Bedford, MA, USA). The cells were cultured in complete medium for 24 h prior to detachment with trypsin EDTA. Subsequently, 5 × 104 cells per chamber were resuspended in culture medium and layered on the Matrigel; after 24 h, the invaded cells were counted with an AMG EVOS Digital Inverted Microscope (Life Technologies, Carlsbad, CA, USA), as described earlier.
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