C57bl 6j

All procedures using animals were approved by the Cleveland Clinic Institutional Animal Care and Use Committee. Mlkl^−/− mice were purchased from Taconic Biosciences (#TF2780) and backcrossed to a C57BL/6J background. WT controls were either C57BL/6J purchased from the Jackson Laboratory or WT littermates. WT and Mlkl^−/− mice were lethally irradiated at 5 weeks of age and then transplanted with bone marrow from Mlkl^−/− or WT mice and allowed to recover for 4 weeks. Mice were then treated with clodronate containing liposomes to deplete resident macrophages in the liver 7 days prior to the start of the Gao‐binge protocol. Mice were allowed free access to a Lieber‐DeCarli liquid diet (Dyets; Cat#710260) containing ethanol at 5% (vol/vol)/28% of total calories or pair‐fed control diet that isocalorically substituted maltose dextrin for ethanol for 10 days. On the final day of the experiment, pair‐fed mice were gavaged with 5 g/kg maltose and ethanol‐fed mice were gavaged with 5 g/kg ethanol in water. Mice were euthanized 6 h after gavage. Additional details of the feeding protocol and tissue collection can be found in Supporting Materials.