Uc6 cm1950

The Foc TR4 was inoculated on the PDA plate, which contained a final concentration of 500 μg/mL extracts. After being cultured at 28°C for 5 days and collected from the edge of the inhibition zone for the Foc TR4 mycelia, the same volume of sterile water was used as a negative control. Samples were fixed, dehydrated, and embedded into Epon 812 resin according to the methods of Cao et al. (2022 (link)). The Foc TR4 mycelia were sliced by an ultra-thin slicer (Leica, UC6 CM1950, Germany). The ultrastructure of the mycelial sample was observed using a transmission electron microscope (TEM, HT7700, Hitachi, Ibaraki, Japan).

Foc TR4 was inoculated on the PDA medium with 500 μg/ml of strain 5–10 extracts at 28°C for 5 days. The Foc TR4 mycelia were fixed with glutaraldehyde (2.5%, v/v) overnight at 4°C and postfixed using osmium tetroxide (1%, v/v). After washing three times with PBS (0.1 mol/L, pH 7.0), the samples were dehydrated with different gradients of ethanol solution and embedded in the Epon 812 resin at 37°C for 12 h, 45°C for 12 h, and 60°C for 24 h, respectively (Xing et al., 2014 ). The Foc TR4 mycelia were sliced by an ultra microtome (Leica, UC6 CM1950, Germany) and stained with uranyl acetate and citric acid for 30 min, respectively. The ultrastructure of transverse Foc TR4 mycelia was detected by TEM (JEM-1400 Flash, Hitachi Limited, Japan).