Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher, USA) respectively supplemented with proteinase and phosphatase inhibitors (Sigma, USA) according to standard protocol. Protein concentrations were determined using the BCA method followed by standard western immunoblotting of proteins using different primary antibodies: Anti-METTL3 (#195352, abcam, USA), Anti-METTL14 (#HPA038002, Sigma, USA), Anti-FTO (#ab124892, abcam, USA), Anti-P21 (#ab109199, abcam, USA), Anti-Phospho-AKT (#9271, Cell Signaling, USA), Anti-AKT (#9272, Cell Signaling, USA), Anti-Phospho ATM (#4526, Cell Signaling, USA), Anti-ATM (#2873, Cell Signaling, USA), Anti-Phospho-IR/IGF1R (#3021, Cell Signaling, USA), Anti-IGF1Rβ (#9750, Cell Signaling, USA), Anti-IRβ (#3025, Cell Signaling, USA), Anti-PDX1 (#5579, Cell Signaling, USA), Anti-Pan-Calcineurin A (#2614, Cell Signaling, USA), Anti-SHP-2 (#3397, Cell Signaling, USA), Anti-β-Actin (#4970, Cell Signaling, USA), Anti-αTubulin (#7291, abcam, USA). (Please see Reporting Summary for further details on antibodies used). The blots were developed using chemiluminescent substrate ECL (ThermoFisher, USA) and quantified using Image studio Lite Ver. 5.2 software (LICOR, USA).
Anti phospho ir igf1r
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-Phospho-IR/IGF1R is a laboratory equipment product that detects phosphorylated insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R) proteins. It is used to monitor the activation status of these signaling receptors in cellular assays.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho akt, by Cell Signaling Technology (2 mentions)
Proteinase and phosphatase inhibitors, by Merck Group (2 mentions)
M per protein extraction reagent, by Thermo Fisher Scientific (2 mentions)
Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (2 mentions)
Anti akt, by Cell Signaling Technology (2 mentions)
Anti mettl14, by Merck Group (2 mentions)
Anti α tubulin, by Abcam (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Anti mettl3, by Abcam (2 mentions)
Anti fto, by Abcam (2 mentions)
Anti p21, by Abcam (2 mentions)
Anti phospho atm, by Cell Signaling Technology (2 mentions)
Anti igf1rβ, by Cell Signaling Technology (2 mentions)
Anti irβ, by Cell Signaling Technology (2 mentions)
Anti pdx1, by Cell Signaling Technology (2 mentions)
Anti pan calcineurin a, by Cell Signaling Technology (2 mentions)
Anti shp2, by Cell Signaling Technology (2 mentions)
Image studio lite ver 5, by Thermo Fisher Scientific (2 mentions)
2 protocols using anti phospho ir igf1r
1
Quantitative Protein Analysis in Cell Lines
2
Quantitative Protein Analysis in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher, USA) respectively supplemented with proteinase and phosphatase inhibitors (Sigma, USA) according to standard protocol. Protein concentrations were determined using the BCA method followed by standard western immunoblotting of proteins using different primary antibodies: Anti-METTL3 (#195352, abcam, USA), Anti-METTL14 (#HPA038002, Sigma, USA), Anti-FTO (#ab124892, abcam, USA), Anti-P21 (#ab109199, abcam, USA), Anti-Phospho-AKT (#9271, Cell Signaling, USA), Anti-AKT (#9272, Cell Signaling, USA), Anti-Phospho ATM (#4526, Cell Signaling, USA), Anti-ATM (#2873, Cell Signaling, USA), Anti-Phospho-IR/IGF1R (#3021, Cell Signaling, USA), Anti-IGF1Rβ (#9750, Cell Signaling, USA), Anti-IRβ (#3025, Cell Signaling, USA), Anti-PDX1 (#5579, Cell Signaling, USA), Anti-Pan-Calcineurin A (#2614, Cell Signaling, USA), Anti-SHP-2 (#3397, Cell Signaling, USA), Anti-β-Actin (#4970, Cell Signaling, USA), Anti-αTubulin (#7291, abcam, USA). (Please see Reporting Summary for further details on antibodies used). The blots were developed using chemiluminescent substrate ECL (ThermoFisher, USA) and quantified using Image studio Lite Ver. 5.2 software (LICOR, USA).
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