Ferrous chloride tetrahydrate

Manufactured by Merck Group

Sourced in Germany, United States, China

Ferrous chloride tetrahydrate is an inorganic compound with the chemical formula FeCl2·4H2O. It is a crystalline solid that appears green in color. The compound's primary function is as a source of ferrous (Fe2+) ions, which are commonly used in various industrial and laboratory applications.

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Lab products found in correlation

Ferric chloride hexahydrate, by Merck Group (79 mentions) Ammonium hydroxide, by Merck Group (19 mentions) Hydrochloric acid (hcl), by Merck Group (14 mentions) Sodium hydroxide (naoh), by Merck Group (13 mentions) Ethanol, by Merck Group (10 mentions) Dimethyl sulfoxide (dmso), by Merck Group (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Methanol, by Merck Group (6 mentions) Ammonium hydroxide solution, by Merck Group (5 mentions) Sodium chloride (nacl), by Merck Group (5 mentions) Potassium permanganate, by Merck Group (5 mentions) Chitosan, by Merck Group (5 mentions) Acetonitrile, by Merck Group (4 mentions) Acetic acid, by Merck Group (4 mentions) Oleic acid, by Merck Group (4 mentions) Sodium nitrate, by Merck Group (4 mentions) Tetraethyl orthosilicate teos, by Merck Group (4 mentions) Ammonia solution, by Merck Group (4 mentions) Formic acid, by Merck Group (4 mentions) Polyvinyl alcohol (pva), by Merck Group (4 mentions)

111 protocols using ferrous chloride tetrahydrate

Extraction and Characterization of Medicinal Plant Oils

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All chemicals, i.e. as ferrous chloride tetrahydrate (FeCl₂·4H₂O ≥ 99%), ferric chloride hexahydrate (FeCl₃·6H₂O, 97%), ammonium hydroxide (25%), isopropanol and n-hexane were obtained from Aldrich Chemical Co. (Missouri, USA) and used without further purification. Saudi Arabia-based heavy crude oil was supplied from the Riyadh refinery unit: Aramco Co. (Riyadh, KSA) and the seawater was collected from the Arabian Gulf along the Saudi coast.
The aerial parts of M. aurea and O. baccatus were collected from the natural area of Rowdah Khuraim (Riyadh, KSA) during March 2016. A taxonomist in the herbarium division of King Saud University identified the species. The plant’s aerial parts were chopped into small pieces and air-dried under shade. After drying, the materials were separately soaked in n-hexane for 72 h thrice. The n-hexane extracts of M. aurea (MAE) and O. baccatus (OBE) were filtered and concentrated under reduced pressure using rotary evaporator.

Abdullah M.M., Atta A.M., Al-Lohedan H.A., Alkhathlan H.Z., Khan M, & Ezzat A.O. (2019). Synthesis of Green Recyclable Magnetic Iron Oxide Nanomaterials Coated by Hydrophobic Plant Extracts for Efficient Collection of Oil Spills. Nanomaterials, 9(10), 1505.

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Biobased Adsorbent Synthesis and Characterization

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Leaves of E. globulus were collected
from precincts of the University of Agriculture, Faisalabad for the
synthesis of extract. Sugar cane bagasse was collected from a local
marketplace of Faisalabad for the preparation of biomass and biochar.
In this research, we employed specific chemicals, namely, ferric chloride
hexahydrate (FeCl₃·6H₂O), ferrous chloride
tetrahydrate (FeCl₂·4H₂O), and sodium hydroxide
(NaOH), which were procured from Aldrich Chemicals. To ensure the
purity of our reagents, we used sterile distilled water sourced from
the biochemistry laboratory, with a conductivity measurement of 1
μS/cm. All the chemicals used in this study met analytical grade
standards and did not require further purification. We meticulously
prepared our solutions using deionized water, and all dilutions were
carried out using double-distilled water.

Zulfiqar N., Nadeem R, & Musaimi O.A. (2024). Photocatalytic Degradation of Antibiotics via Exploitation of a Magnetic Nanocomposite: A Green Nanotechnology Approach toward Drug-Contaminated Wastewater Reclamation. ACS Omega, 9(7), 7986-8004.

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Synthesis of Iron Oxide Nanoparticles

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Ferric chloride hexahydrate (FeCl₃·6H₂O), ferrous chloride tetrahydrate (FeCl₂·4H₂O), Calcium chloride dihydrate (CaCl₂.2H₂O), diammonium hydrogen phosphate ((NH₄)₂(HPO₄)), Cetyl trimethylammonium bromide (CTAB), ammonium hydroxide (NH₄OH) and the other regents all were purchased locally from Sigma-Aldrich Chemicals and used as received.

Kermanian M., Naghibi M, & Sadighian S. (2020). One-pot hydrothermal synthesis of a magnetic hydroxyapatite nanocomposite for MR imaging and pH-Sensitive drug delivery applications. Heliyon, 6(9), e04928.

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Functionalized Alginate Hydrogels for Tissue Engineering

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Sodium alginate (LVG, 100 kDa, >65% guluronic acid) was obtained from NovaMatrix FMC Biopolymers (Drammen, Norway). Ferric chloride hexahydrate, ferrous chloride tetrahydrate, D-gluconic acid, hemicalcium salt and sodium hydroxide were obtained from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffered solution (PBS) and Dulbecco’s modified eagle’s medium (DMEM) supplemented with L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate was obtained from MediaTech (Manassas VA, USA), BenchMark^TM heat inactivated fetal bovine serum (FBS) was obtained from Gemini Bio-Products (West Sacramento, CA USA). Deionized water used in all experimental procedures was obtained using a Milli-Q water purification system. Alginate was modified with functional peptides RGD and heparin binding peptide (HBP) as was previously described^{28 (link)}.

Sapir-Lekhovitser Y., Rotenberg M.Y., Jopp J., Friedman G., Polyak B, & Cohen S. (2016). Magnetically actuated tissue engineered scaffold: insights into mechanism of physical stimulation. Nanoscale, 8(6), 3386-3399.

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Synthesis and Characterization of Iron Compounds

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Ferric chloride hexahydrate (FeCl₃.6H₂O, ≥99%), Ferrous chloride tetrahydrate (FeCl₂.4H₂O, 99.99%), Ammonium hydroxide (NH₄OH, 28.0–30.0%), Sodium chloride ≥99.5%, Hydrochloric acid 37%, Sodium nitrate (NaNO₃, ≥99.0%), Potassium permanganate (KMnO₄, ≥99.0%) were purchased from Sigma-Aldrich.

Thapa B., Diaz-Diestra D., Badillo-Diaz D., Sharma R.K., Dasari K., Kumari S., Holcomb M.B., Beltran-Huarac J., Weiner B.R, & Morell G. (2019). Controlling the transverse proton relaxivity of magnetic graphene oxide. Scientific Reports, 9, 5633.

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Synthesis of Multifunctional Nanoparticles

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Ferric chloride hexahydrate (98%), ferrous chloride tetrahydrate, and triethoxy-3-(2-imidazoline-1-yl)propylsilane (TIP; 97%) were purchased from Sigma-Aldrich (USA). Ammonia solution (25%), ethanol (98%), CA (Ph Eur), tetraethyl orthosilicate (TEOS, 98%), cisplatin (Cis-Pt), dimethylformamide, toluene, tetrahydrofuran, acetic acid, sodium acetate, sodium chloride, potassium dihydrogen phosphate, and dipotassium hydrogen phosphate were supplied by Merck (Germany). Hexadecyltrimethylammonium bromide (CTAB) was purchased from Daejung Chemicals (South Korea). All chemicals were of reagent grade and used as received.

Abedi M., Abolmaali S.S., Abedanzadeh M., Farjadian F., Mohammadi Samani S, & Tamaddon A.M. (2020). Core–Shell Imidazoline–Functionalized Mesoporous Silica Superparamagnetic Hybrid Nanoparticles as a Potential Theranostic Agent for Controlled Delivery of Platinum(II) Compound. International Journal of Nanomedicine, 15, 2617-2631.

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Membrane Fabrication and Alloy Synthesis

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The polymers used for membrane preparation included cellulose acetate (average Mn ~50,000 by GPC), obtained from Aldrich (Munich, Germany), and poly(methylemethacrylate), purchased from Lanxess (Liyang, China). The solvent mixture used included 1,4-dioxan (99.5%), purchased from Adwic, El-Nasr Pharmaceutical Chemicals Company (Qalyubia, Egypt); acetone (99%), obtained from El-salam for Chemical Industries (Giza, Egypt); methanol (99.8%, HPLC grade), obtained from Fisher (Loughborough, UK); and acetic acid (99.8% for biochemistry), purchased from Acros Organics (New Jersey, USA). Diethyle phthalate (99%) was obtained from Loba Chemie (Mumbai, India). Lithium chloride anhydrous was purchased from Fisher (Loughborough, UK). The chemicals used for alloy synthesis included nickel chloride hexahydrate (NiCl₂·6H₂O, 98%) and ferrous chloride tetrahydrate (FeCl₂·4H₂O, 99.99%) as sources of metal ion, which were purchased from Sigma (Darmstadt, Germany). Hydrazine hydrate (N₂H₄·H₂O, 99%) was obtained from Fisher (Horsham, UK) as a reducing agent. Sodium hydroxide (NaOH, 98%) catalyst was purchased from Trading Dynamic Co. (TDC, Cairo, Egypt). Distilled water was used as a solvent.

Nady N., Salem N., Elmarghany M.R., Salem M.S, & Kandil S.H. (2022). Novel Magnetic Mixed Cellulose Acetate Matrix Membranes with Oxygen-Enrichment Potential. Membranes, 12(12), 1259.

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Synthesis of Graphene Oxide and Nanoparticles

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All chemicals were of analytical grade and used as received without further purification. Graphite flakes (~105 µm flakes), potassium permanganate (KMnO₄), aspartic acid, sodium nitrate (NaNO₃), gold(III) chloride trihydrate (HAuCl₄.₃H₂O) Sodium hydroxide (NaOH), Ferrous chloride tetrahydrate (FeCl₂·4H₂O) were supplied by Sigma Aldrich.

Bharath G., Anwer S., Mangalaraja R.V., Alhseinat E., Banat F, & Ponpandian N. (2018). Sunlight-Induced photochemical synthesis of Au nanodots on α-Fe2O3@Reduced graphene oxide nanocomposite and their enhanced heterogeneous catalytic properties. Scientific Reports, 8, 5718.

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Synthesis of Magnetic Polymer Nanoparticles

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All reagents were of analytical grade and used as received without any purification: Ferric chloride hexahydrate (FeCl₃.6H₂O, 97%), ferrous chloride tetrahydrate (FeCl₂.4H₂O, 99%), ammonium hydroxide (NH₄OH_(ag), 28–30%), tetraethylorthosilicate (TEOS, 98%), vinyltrimethoxysilane (VTMS, 98%), toluene (99.9%), magnesium sulfate (MgSO₄, 99.5%), N-vinylcaprolactam (NVCL, 98%), methacrylic acid (MAA, 99%), ammonium persulfate (APS, 98%), N, N’-Methylenebisacrylamide (NMBS, 99%), Doxorubicin hydrochloride (DOX, 98–102%) Alsever´s solution, and phosphate buffered saline solution (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol (99%) and isopropyl alcohol (99%) were purchased from JT Baker (Phillipsburg, NJ, USA).

Sánchez-Orozco J.L., Meléndez-Ortiz H.I., Puente-Urbina B.A., Rodríguez-Fernández O.S., Martínez-Luévanos A, & García-Cerda L.A. (2023). Synthesis and Characterization of a pH- and Temperature-Sensitive Fe3O4-SiO2-Poly(NVCL-co-MAA) Nanocomposite for Controlled Delivery of Doxorubicin Anticancer Drug. Polymers, 15(4), 968.

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Multifunctional Fe3O4@Silica Nanoparticles for RNA Extraction

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To synthesize the Fe₃O₄@Silica for RNA extraction, the following chemicals of analytical reagent grade were received and used without further purification. Ferric chloride hexahydrate (FeCl₃·6H₂O), ferrous chloride tetrahydrate (FeCl₂·4H₂O), styrene, glycidyl methacrylate (GMA), divinylbenzene (DVB), azobisisobutyronitrile (AIBN), polyacrylic acid, guanidine hydrochloride (>99%) and ethanol anhydrous were obtained from Sigma Aldrich. Ammonia in water 28–30% w/w, sodium citrate tribasic dihydrate, nitric acid (90%) were obtained from BDH. Tetraethyl Orthosilicate (TEOS), 3-glycidyloxypropyltrimethoxysilane (GTMOS), (3-Aminopropyl)triethoxysilane (APTES) were purchased from TCI (USA). All aqueous solutions were prepared with deionized water obtained from a Milli-Q Integral system (resistivity = 18 MΩ cm, EMD Millipore). Zymo MagBinding® Beads was obtained from Zymo Research, USA for comparison in RNA binding. Dynabeads® MyOne^TM, M280 from Thermofisher were used as the microscopic reference sizing materials, employed at 800x magnification.

Lee A.H., Gessert S.F., Chen Y., Sergeev N.V, & Haghiri B. (2018). Preparation of iron oxide silica particles for Zika viral RNA extraction. Heliyon, 4(3), e00572.

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