Methyl alcohol

The chemicals e.g. methyl alcohol, 2-propyl alcohol, ethyl alcohol, Sodium borohydride, resazurin sodium salt powder (resazurin) and tri-sodium citrate, were supplied by Sigma-Aldrich (St. Louis, MO) unless otherwise stated. Tetraethyl-orthosilicate (TEOS), cetyl-trimethyl-ammonium bromide (CTAB), Hydrogen tetrachloroaurate (III) trihydrate (HAuCl4·3H2O, 99%) was obtained from Acros Organics (Geel, Belgium). N-(amino-ethyl)-amino-propyl trimethoxysilane (TSD) was supplied by ABCR GmbH y Co.KG. All other chemicals were of the uppermost quality and used as received. Milli-Q water was used in all experiments.

For the synthesis of GO, graphite powders (carbon basis: 99.0%, particle size: ~325 mesh), potassium persulfate (99.0%), and phosphorus pentoxide (99.0%) were purchased from Sigma-Aldrich (St. Louis, MO, US) and potassium permanganate (99.3%) was purchased from Junsei Chemical (Tokyo, Japan). Sulfuric acid (95.0%), hydrogen peroxide (30.0%), and hydrochloric acid (35.0%) were purchased from Daejung Chemical (Si-heung, Korea). DCX (98.0%) and methyl alcohol (99.5%), purchased from Sigma-Aldrich (St. Louis, MO, US) and Samchun (Seoul, Korea), respectively, were employed for the crosslinking of the GO laminates. All of the organic dyes (rose bengal (RB, 1017 g mol⁻¹), methyl blue (MB, 800 g mol⁻¹), acid fuchsin (AF, 585.5 g mol⁻¹), rhodamine B (RhB, 479 g mol⁻¹), and methyl orange (MO, 327 g mol⁻¹)) and ionic salts (sodium sulfate anhydrous (Na₂SO₄, >99.0%), sodium chloride (NaCl, >99.5%), magnesium sulfate anhydrous (MgSO₄, >99.0%), and magnesium chloride hexahydrate (MgCl₂·6H₂O, >98.0%)) were purchased from Daejung Chemical (Si-heung, Korea). A nylon membrane filter was purchased from SciLab Korea Co., Ltd (Seoul, Korea) to synthesize GO. Other nylon filter (diameter: 47 mm, Millipore filters with a pore size of 0.2 μm; Saint-Quentin, France) was used as the support layers of the GO membranes.

AFB₁ (2, 3, 6α, 9α-tetrahydro-4-methoxycyclopenta [c] furo [2, 3:45] furo [2, 3-h] chromene-1, 11-dione; purity >98%) was obtained from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Methyl alcohol, dimethyl sulfoxide (DMSO), and other reagents are analytical reagents and purchased from Sinopharm (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) Peanut oil was purchased from Wal-Mart supermarket in Wuhan, China.
For high-performance liquid chromatography (HPLC) and TQEF-MS/MS analysis, chromatographic grade acetonitrile and Methyl alcohol were purchased from Sigma (St. Louis, MO, USA). The deionized water was obtained from a Milli-Q SP Reagent Water system (Millipore, Bedford, MA, USA).
For cytotoxicity tests, human embryo hepatocytes (L-02 cells) were purchased from Procell (Procell Co. Ltd., Wuhan, China). Culture medium RPMI-1640 and fetal calf serum were purchased from HyClone ((GE-Healthcare, Logan, UT, USA). The 0.05% trypsin solution was from Thermo Fisher (Thermo Fisher Scientific, Waltham, MA, USA).

In this work, UV absorbance spectra of the naproxen acid, naproxen sodium salt, and pharmaceutical preparations containing NAS (NA1-NA4) and NSS (NS1-NS5) as the active pharmaceutical ingredients were registered. For this purpose, 10 mg of each of the samples were dissolved in 100 mL of 96% methyl alcohol (Sigma-Aldrich Company, St. Louis, MO, USA). The samples were then mixed and poured into a quartz cuvette placed in a UV-Vis spectrophotometer. UV absorbance spectra were obtained in the wavelength range from 245 nm to 345 nm. The UV-Vis spectrophotometer Thermo Genesys 10S produced by Thermo Scientific (Waltham, MA, USA) was used. To obtain and analyze UV spectra, VisionLite Software (Thermo Scientific Company, Waltham, MA, USA) and Origin 2016 (OriginLab Company, Northampton, MA, USA) were used.

AFM₁ standard solutions (50 µg mL⁻¹ in acetonitrile) were obtained from (Sigma-Aldrich, Dorset, UK). HPLC-grade acetonitrile and methyl alcohol were purchased from (Sigma-Aldrich, Steinheim, Germany). Raw milk samples were acquired from local farms in various regions of Alexandria Governorate, Egypt. The preliminary milk sample was prepared in aliquots of 50 mL and then stored at −20 °C for further analysis.

Phosphoric acid (85 wt. % in H₂O), urea (99.0–100.5%), hydrochloric acid (37%), sodium thiosulfate (99.9%), iodine (≥99.99%), methyl alcohol (≥99.9%), ethyl alcohol (95.0%) and isopropyl alcohol (≥99.5%) used in this study were of reagent grade and purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium chlorite (80%, pure) and chlorophenol red (pure) were also of reagent grade and acquired from Acros Organics (Waltham, MA, USA). All solutions were prepared in deionized, triply distilled water (specific resistance of 18.0 Ω m).