Anti f4 80 antibody

Manufactured by Novus Biologicals

The Anti-F4/80 antibody is a laboratory reagent used for the detection and identification of the F4/80 antigen, which is expressed on the surface of macrophages and related cell types. This antibody can be used in various immunological techniques, such as flow cytometry and immunohistochemistry, to study the presence and distribution of macrophages in biological samples.

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Lab products found in correlation

Histofine simple stain max po, by Nichirei Biosciences (1 mentions) Anti il 1β antibody, by Abcam (1 mentions) Anti il 18 antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

F4/80 (8 citations) Anti-F4/80 (5 citations)

2 protocols using anti f4 80 antibody

Adipose Tissue Histological Analysis

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Inguinal subcutaneous adipose tissue soaked in 4% paraformaldehyde was embedded in paraffin and cut into thin sections. The sections were mounted on slides, deparaffinized in xylene, and stained with an anti-F4/80 antibody (Novus Biologicals, Littleton, CO). After incubation with Histofine Simple Stain MAX-PO (Nichirei, Tokyo, Japan), color was developed with 3,3′-diaminobenzidine tetrahydrochloride. Sections were rinsed and counterstained with Mayer’s hematoxylin. Skin samples were collected and fixed with 4% paraformaldehyde, embedded in paraffin, and cut into thin sections. Paraffin sections were analyzed by hematoxylin and eosin (H/E) staining.

Aoki M, & Murase T. (2019). Obesity-associated insulin resistance adversely affects skin function. PLoS ONE, 14(10), e0223528.

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Kidney Tissue Immunohistochemistry Protocol

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Kidneys were embedded with paraffin; 5-μm sections were cut and mounted onto microscope slides. After heat-induced antigen retrieval, washing with 3% hydrogen peroxide, and 30-min blocking with fetal bovine serum, slides were incubated with anti-IL-1β antibody (1:100; Abcam Biotechnology, Cambridge, MA), anti-IL-18 antibody (1:100; Santa Cruz Biotechnology, Dallas, TX), and anti-F4/80 antibody (1:100; Novus Biologicals, Littleton, CO) and then diluted in PBS with 4% fetal bovine serum overnight. The sections were washed with PBS and incubated with biotinylated IgG (1:200) for 1 h at room temperature, then with streptavidin-HRP for 30 min. Each kidney section was then stained with DAB for 1 min followed by counterstaining with hematoxylin for 5 min. The slides were mounted and observed under a microscope [47 (link)].

Huang D., Kidd J.M., Zou Y., Wu X., Gehr T.W., Li P.L, & Li G. (2023). Regulation of NLRP3 Inflammasome Activation and Inflammatory Exosome Release in Podocytes by Acid Sphingomyelinase During Obesity. Inflammation, 46(5), 2037-2054.

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