Prism 5 data analysis software

Results were analyzed for statistical significance (defined as p<0.05 and indicated by asterisks in figures) by performing unpaired, two-sided Student's t-test with GraphPad Prism 5 Data Analysis Software (GraphPad Software, Inc., La Jolla, CA). Mean and standard error of mean (SEM) values were calculated from at least three biologically and technically independent experiments.

Growth curves were performed as previously described^{23 (link)}. Briefly, the indicated cell lines were grown exponentially in 8-mL tubes with different μM concentrations of bLF or bLFcin (described in the corresponding figure legends) and cellular proliferation/viability measured at 6-, 12-, 24- and 48-h after treatment. The number of viable cells was determined by counting with a haemocytometer after addition of 0.4% Trypan blue, according to the instructions from the manufacturer. Results were analysed for statistical significance by two-way ANOVA with GRAPHPAD Prism 5 Data Analysis Software (GRAPHPAD Software Inc., La Jolla, CA, USA). Mean and standard mean values were calculated from at least three biologically and technically independent experiments. p < 0.001 was considered significant and was indicated by asterisks in the figures.