Electroporator
The Eppendorf Electroporator is a device used for the electroporation of cells, a technique that introduces foreign molecules into cells by creating temporary pores in their membranes using electrical pulses. The device allows users to set and control the electrical parameters, such as voltage, pulse duration, and number of pulses, to optimize the electroporation process for different cell types and applications.
Lab products found in correlation
13 protocols using electroporator
Heterologous expression of epothilone in M. xanthus
Genetic Transformation of Sugary-1 Mutant Rice
Recombinant BCG Vaccine Production
Fluorinated Tyrosine Incorporation in GFP
Fluorotyrosine Incorporation in GFP
Expression and Purification of cHSPA6
Construction and Screening of Aa113 BAC Library
Somatic Cell Nuclear Transfer in Oocytes
Plasmid Transformation of M. extorquens AM1
Optimizing GNPs Uptake in HT-29 Cells
400 µl cell suspensions incubated with 60 µM of GNPs and was added into a sterile electroporation cuvette (Eppendorf, Netherlands) with a 4-mm gap between the electrodes.
The cell/GNPs mixture of the cuvette was incubated for 10 min at 4 °C on ice and then transferred to an electroporation chamber.
Number of four electric pulses of different voltages 200, 400, 600, 800, 1000 and 1200 with duration of 100 µs and pulse number
of 2 were delivered using an Electroporator (Eppendorf). Field amplitudes higher than 1200 V/cm are not normally tested as it is known that they are highly
toxic for the cells (induction of irreversible electroporation and, thus, of cell death). Immediately after electroporation, the cuvette was taken
out from the chamber and incubated for 2 min at 4 °C on ice and then cells were stored at humidified atmosphere at 37 °C with 5% CO2for 3-4 hr before viability and uptake assay processes using GFAAS analysis as described above.
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