Alexa fluor 568 anti goat igg

Manufactured by Thermo Fisher Scientific

Sourced in United States

Alexa Fluor 568 anti-Goat IgG is a secondary antibody conjugated with the Alexa Fluor 568 fluorescent dye. It is designed for the detection and visualization of goat primary antibodies in various immunoassays and imaging applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Alexa Fluor 568 goat anti-rabbit IgG (H+L) Goat anti-rabbit IgG (H + L) Alexa Fluor® 488 and 568 Goat anti-mouse IgG (H + L) Alexa Fluor® 488 and 568 Alexa Fluor 568 goat anti-rabbit immunoglobulin G (IgG; Alexa Fluor® 568 conjugated goat anti-rabbit IgG secondary antibody Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody Alexa Fluor 568 goat anti-mouse IgG (A11031) Alexa Fluor 568 donkey anti-goat IgG Alexa Fluor 568-conjugated goat anti-rabbit IgG(H+L) Donkey anti-Goat IgG (H+L) Alexa Fluor 568

7 protocols using alexa fluor 568 anti goat igg

Multimodal Analysis of Brain Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thick (100-200um) brain sections were prepared with vibratome and stored
in tissue freezing media (25% Glycerol, 30% Ethylene glycol, 1.38g/L
NaH₂PO₄, 5.48g/L Na₂HPO₄). The
primary antibodies used in this study as follows; anti-RFP/ tdTomato (MBS448092;
MyBioSource), anti-GFAP (Z0334; DAKO), anti-NeuN (MAB377, MilliporeSigma),
anti-Olig2 (AB9610, MilliporeSigma), anti-Sox2 (14-9811-80; eBioscience). The
secondary antibodies were as follows (all from Invitrogen): Alexa Fluor 568
anti-goat IgG, Alexa Fluor 647 anti-rat IgG, Alexa Fluor 647 anti-rabbit IgG,
Alexa Fluor 647 anti-mouse IgG. The nucleus was stained with DAPI.

Hara T, & Verma I.M. (2019). Modeling Gliomas Using Two Recombinases. Cancer research, 79(15), 3983-3991.

+ Open protocol

+ Expand

Lung Tissue Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

To stain the lung tissues, the lungs were inflated with Tissue-Tek optimal cutting temperature (OCT) compound (Sakura, Tokyo, Japan) diluted 1:4 in PBS, subsequently embedded in OCT compound, and frozen at −80°C. Frozen sections (12 μm) of the isolated lung specimens were prepared, fixed, and permeabilized with the BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit (BD Biosciences) in accordance with the manufacturer's instructions. The lung specimens were incubated overnight at 4°C with purified anti-mouse IL-33 antibodies (clone Poly5165; BioLegend) and anti-pro surfactant protein C (proSP-C) antibodies (EMD Millipore; Merck KGaA, Darmstadt, Germany). After that, we stained them with Alexa Fluor®568 anti-goat IgG and Alexa Fluor®647 anti-rabbit IgG (Invitrogen, San Diego, CA, USA) for 1 h and co-stained them with DAPI (Sigma Aldrich, St. Louis, MO, USA) for 20 min. Images were analyzed with a FluoView (FV10i) confocal microscope (Olympus, Tokyo, Japan).

Kobari S., Kusakabe T., Momota M., Shibahara T., Hayashi T., Ozasa K., Morita H., Matsumoto K., Saito H., Ito S., Kuroda E, & Ishii K.J. (2020). IL-33 Is Essential for Adjuvant Effect of Hydroxypropyl-β-Cyclodexrin on the Protective Intranasal Influenza Vaccination. Frontiers in Immunology, 11, 360.

+ Open protocol

+ Expand

Whole-Mount Immunohistochemistry for Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-mount immunohistochemistry was performed using a modification of
the protocol from Ahnfelt-Rønne et al.
(2007) as previously described (Havrilak and Shannon, 2015b (link)). Optical sections were captured on a
Nikon AiRsi inverted laser microscope, and 3D images were created by a composite
of z-stacks using Bitplane Imaris software to process the fluorescent images. To
calculate the volume of tissue stained for ALB, we created isosurfaces using
smoothing thresholds and intensity values that allowed filtering out antibody
aggregates or any non-specific staining.
The primary antibodies used were: rabbit anti-NKX2-1 (1:3000, Seven
Hills Bioreagents), guinea pig anti-NKX2-1 (1:500, Seven Hills Bioreagents),
goat anti-endomucin (1:500, R & D Systems), rat anti-E-cadherin (1:2000,
R & D Systems), rabbit anti-PAX8 (1:500, Protein Tech), rat anti-PECAM-1
(1:500, BD Pharmigen), goat anti-HNF4α (1:200, Santa Cruz), goat
anti-PDX1 (1:5000, Abcam), goat anti-ALB (1:1000, Bethyl Laboratories), goat
anti-FOXA2 (1:500, Santa Cruz), and rat anti-LIV2 (1:200, MBL). Secondary
antibodies used were: Alexa Fluor 647 donkey anti-rabbit IgG, Alexa Fluor 568
anti-goat IgG, Alexa Fluor 488 donkey anti-rat IgG, Alexa Fluor 488 donkey
antimouse IgG (1:500, all from Life Technologies).

Havrilak J.A., Melton K.R, & Shannon J.M. (2017). Endothelial cells are not required for specification of respiratory progenitors. Developmental biology, 427(1), 93-105.

+ Open protocol

+ Expand

Whole-Mount Immunohistochemistry for Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Havrilak J.A., Melton K.R, & Shannon J.M. (2017). Endothelial cells are not required for specification of respiratory progenitors. Developmental biology, 427(1), 93-105.

+ Open protocol

+ Expand

Multimodal Analysis of A2B5 and OLIG2

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed using MACSQuant^® 10 (Miltenyi Biotec SAS, Paris, France) on dissociated cells using A2B5 (mouse APC-IgM, clone 105, Miltenyi Biotec SAS) following the manufacturer’s instructions. Data were analyzed using FlowJo software (10.8.1, Becton Dickinson & Company, Tree Star, Inc., Ashland, OR, USA).
For immunofluorescence microscopy, U87-ST8sia3 cells were grown as a monolayer on glass coverslips in 24-well plates in DMEM with 10% FCS. The primary antibodies A2B5 (mouse IgM, clone 105, 1/1000, kindly provided by G. Rougon, Marseille, France) and OLIG2 (goat IgG, 1/50, Bio-Techne SAS, Noyal Châtillon sur Seiche, France) were incubated for 1 h at room temperature followed by fluorochrome-conjugated secondary antibodies Alexa fluor 568 anti-mouse IgM and Alexa fluor 568 anti-goat IgG (Molecular Probes, Eugene, OR, USA) incubated at 2 μg/mL for 1 h at room temperature together with Hoechst 33342 (1/1000, Merck, Saint Quentin Fallavier, France). All the images were obtained using a Zeiss AXIO-Observer Z1 microscope (Carl Zeiss SAS, Marly-le-Roi, France).

Baeza-Kallee N., Bergès R., Hein V., Cabaret S., Garcia J., Gros A., Tabouret E., Tchoghandjian A., Colin C, & Figarella-Branger D. (2023). Deciphering the Action of Neuraminidase in Glioblastoma Models. International Journal of Molecular Sciences, 24(14), 11645.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Glioblastoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

U251-MG and U87-MG cell lines were grown as a monolayer on glass coverslips in 24-well plates in DMEM with 10% FCS. For GBM CSCs, immunofluorescence experiments were performed on 8-day-old spheres or on cells cultured on poly-DL-ornithin as explained above. Primary antibodies A2B5 (mouse IgM, clone 105, 1/1000, kindly provided by G. Rougon, Marseille, France), anti-ST8SIA3 (rabbit IgG, 1/1000, Sigma-Aldrich), and anti-Ki67 (mouse IgG, clone Mib1, Dako, Les Ulis, France) were incubated for 1 h at room temperature. Fluorochrome-conjugated secondary antibodies Alexa fluor 568 anti-mouse IgM, Alexa fluor 488 anti-rabbit IgG, Alexa fluor 568 anti-mouse IgG, or Alexa fluor 568 anti-goat IgG (Molecular Probes, Eugene, OR, USA) were incubated at 2 µg/mL for 1 h at room temperature together with Hoechst 33342. All the images were obtained using a Zeiss AXIO-Observer Z1 microscope (Carl Zeiss SAS, Marly-le-Roi, France). Conventional immunohistochemistry for cleaved caspase-3 (C92-605 clone, 1/500, BD Biosciences, Franklin Lake, NJ, USA ) and Ki67 (30-9 clone, Ventana Medical Systems, Illkirch, France) was performed using the Benchmark XT automate (Ventana Medical Systems) according to the manufacturer’s instructions. Sections without primary antibody served as a negative control.

Baeza-Kallee N., Bergès R., Soubéran A., Colin C., Denicolaï E., Appay R., Tchoghandjian A, & Figarella-Branger D. (2019). Glycolipids Recognized by A2B5 Antibody Promote Proliferation, Migration, and Clonogenicity in Glioblastoma Cells. Cancers, 11(9), 1267.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells grown on a 35 mm Glass Base dish (IWAKI, Chiba, Japan) were washed twice with D-PBS and fixed with 100% methanol for 20 min at room temperature. Cells were permeabilized with detector blocking solution for CTL1 detection or iBind^TM Flex Solution for CTL2 detection overnight at 4°C. Cells were incubated with anti-CTL1 polyclonal antibody (2 μg/ml), anti-CTL2 monoclonal antibody (2 μg/ml), anti-COX IV polyclonal antibody (2 μg/ml) and anti-Na⁺/K⁺-ATPase monoclonal antibody (5 μg/ml) in new blocking solution for 4 h at room temperature. After being washed with wash solution, the cells were incubated with 2 μg/ml Alexa Fluor 488 anti-rabbit IgG (Molecular Probes Inc.) and 2 μg/ml Alexa Fluor 568 anti-goat IgG for 1 h at room temperature. After excess antibody was washed out with wash solution, the specimens were mounted using VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA). Fluorescence images were obtained with a Confocal Laser Scanning Biological Microscope (FV10i-DOC, Olympus, Tokyo, Japan).

Nagashima F., Nishiyama R., Iwao B., Kawai Y., Ishii C., Yamanaka T., Uchino H, & Inazu M. (2017). Molecular and Functional Characterization of Choline Transporter-Like Proteins in Esophageal Cancer Cells and Potential Therapeutic Targets. Biomolecules & Therapeutics, 26(4), 399-408.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!