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Triton x 100

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Triton X-100 is a non-ionic detergent commonly used in laboratory settings. It is a clear, viscous liquid that functions as a surfactant, reducing the surface tension of aqueous solutions. Triton X-100 is primarily utilized for the solubilization and extraction of membrane proteins, as well as for cell lysis and protein purification applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (4 mentions) Dimethyl sulfoxide (dmso), by HiMedia (4 mentions) Ethylenediaminetetraacetic acid, by HiMedia (3 mentions) Disodium hydrogen phosphate, by HiMedia (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Sodium chloride (nacl), by HiMedia (3 mentions) Sodium hydroxide (naoh), by HiMedia (3 mentions) Fetal bovine serum (fbs), by HiMedia (3 mentions) Trypsin edta solution, by HiMedia (2 mentions) Tween 20, by Merck Group (2 mentions) Paraformaldehyde (pfa), by HiMedia (2 mentions) Ethanol, by HiMedia (2 mentions) Potassium dihydrogen phosphate, by HiMedia (2 mentions) Trypsin, by HiMedia (2 mentions) Hydrogen peroxide, by HiMedia (2 mentions) Lead citrate, by Merck Group (1 mentions) Uranyl acetate, by Merck Group (1 mentions) Atp determination kit, by Thermo Fisher Scientific (1 mentions) Osmium tetroxide, by Merck Group (1 mentions) Epoxy embedding medium kit, by Merck Group (1 mentions)

31 protocols using triton x 100

1

Immunofluorescence Staining of ELF3 in Cells

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Approximately 3X10 3 cells were seeded in chamber vials and grown for 48 hours in humidified 5% CO2. Cells were fixed with 4% Paraformaldehyde (PFA) (Merk, India) and then permeabilised with 0.5% Triton-X100 (HiMedia, India). Cells were then blocked with 0.1% Triton-X100 in 3% BSA (HiMedia, India). Samples were stained with primary antibody ELF3 (R&D systems), and secondary antibodies -Alexa Fluor® 568-labeled donkey anti-goat IgG (A11057, Invitrogen, CA, USA) and DAPI (D1306, Invitrogen, CA, USA). Images were acquired under inverted epifluorescent microscope (Olympus IX73, USA).
Gondkar K., Patel K., Krishnappa S., Patil A., Nair B., Sundaram G.M., Zea T.T, & Kumar P. (2019). E74 like ETS transcription factor 3 (ELF3) is a negative regulator of epithelial- mesenchymal transition in bladder carcinoma. Cancer biomarkers : section A of Disease markers, 25(2).
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2

Comprehensive Cell Culture Protocols

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Chemicals such as Dulbecco’s Modified Eagle’s Medium (DMEM), l-Glutamine, Fetal Bovine Serum (FBS), Trypsin-EDTA solution, Antibiotic-Antimycotic solution (10000 U/mL Penicillin, 10 mg/mL Streptomycin and 25 μg/mL Amphotericin B in 0.9 % normal saline for 100 X), MTT dye, Dimethyl Sulfoxide (DMSO), Trypan blue, Dulbecco’s Phosphate Buffered Saline (DPBS), Tris-EDTA, Propidium iodide (PI), Ribonuclease A (RNase A) and Triton X-100 were purchased from Himedia, India. Molecular probes, Fluorescein isothiocyanate-Phalloidin (FITC-Phalloidin), and 4′,6- diamidino-2-phenylindole (DAPI) were obtained from ThermoFisher Scientific, USA. DCFDA, 3,3′-dihexyloxacarcocyanine iodide (DiOC6), Rotenone, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, disodium phosphate (Na2HPO4) 25 % (v/v) glutaraldehyde, paraformaldehyde, osmium tetroxide, uranyl acetate, and lead citrate were purchased from Sigma Aldrich, USA. The kits used in this study such as the SOD Assay kit and Epoxy Embedding Medium Kit were procured from Sigma Aldrich, USA (Cat. No.:19106 and Cat. No.: 45359-1EA-F respectively), and the ATP Determination kit was purchased from Thermo Scientific, USA (Cat. No.: A22066). The absolute ethanol used in this study is of HPLC grade (Commercial Alcohols, Greenfield Global, Canada). All the reagents and chemicals are of analytical grade.
Kar N., Gupta D, & Bellare J. (2021). Ethanol affects fibroblast behavior differentially at low and high doses: A comprehensive, dose-response evaluation. Toxicology Reports, 8, 1054-1066.
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3

Evaluation of Antioxidant Enzymes in Olive Oil-treated Rats

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Vitamin C purified/ascorbic acid (Merck Specialities Private Limited., Mumbai, India), carbon tetrachloride (CCl4) (Rankem Fine Chemicals Limited, New Delhi, India), Refined olive oil (SOS Cuetara, S. A, Madrid, Spain) were obtained. Other chemicals used were 2-thiobarbituric acid, 5, 5’-dithio bis (2-nitro-benzoic acid) (Sigma — Aldrich Co., USA), bovine albumin fraction-V, ethylene diamine tetra acetic acid, di-sodium salt, Copper (II) sulfate, pentahydrate, potassium sodium tartarate, tetrahydrate, triton X-100 (HiMedia Laboratories Pvt. Ltd., Mumbai, India), pyrogallol, potassium dihydrogen orthophosphate (Qualigens Fine Chemicals, Mumbai India), tris buffer, hydrogen peroxide solution 30%, potassium dihydrogen phosphate, di-sodium hydrogen orthophosphate, hydrochloric acid, trichloro acetic acid, and methanol (Ranbaxy Fine Chemicals Limited, New Delhi, India). All other chemicals and reagents used were of analytical grade.
Joseph J.A., Radhakrishnan U., Mutyala S., Goudar K.S., Ayyappan U.P, & Agarwal A. (2015). Antioxidant and protective effects of Phytocee™ against carbon tetrachloride-induced oxidative stress. Journal of Natural Science, Biology, and Medicine, 6(1), 183-187.
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4

Immunofluorescence Imaging of Mammalian Cells

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For imaging the mammalian cells, after appropriate treatments, coverslips containing cells were fixed using 4% paraformaldehyde (PFA; Sigma) and then permeabilized using Triton X-100 (0.2%, HiMedia). Coverslips were mounted on slide using antifade, Vectashield mounting medium (Vector Laboratories). For antibody staining, coverslips were blocked using 5% BSA for 1 h at room temperature, then incubated with primary antibody at 4°C, overnight and then subsequently probed with corresponding fluorescent dye conjugated secondary antibody.
Images were acquired using DeltaVision Elite widefield microscope (API, GE) with following filters: FITC (490/20 and 529/38), TRITC (542/27 and 594/45) and Cy5 (632/22 and 676/34). Acquired images were processed using DV softWoRX software.
Suresh S.N., Chavalmane A.K., Pillai M., Ammanathan V., Vidyadhara D.J., Yarreiphang H., Rai S., Paul A., Clement J.P., Alladi P.A, & Manjithaya R. (2018). Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective. Frontiers in Molecular Neuroscience, 11, 109.
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5

Methylglyoxal-Based Immunoassay Protocol

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Methylglyoxal (MG), anti-human IgG alkaline phosphatase conjugates, p-nitrophenyl phosphate, sodium dodecyl sulfate, Tween-20, Protein A-agarose (2.5 ml pre-pack column) and dialysis tubing were purchased from Sigma Chemical Company, U.S.A. Dihydroxy acetone (DHA) was from Merck, Germany. Lysine was from Sisco research laboratory. Triton X-100 was procured from Hi–Media. Trizma base was from Spectrochem, Mumbai, India. ELISA plates (96 wells) were purchased from NUNC, Denmark. Acrylamide, bisAcrylamide, ammonium persulphate and N,N,N′,N′-tetramethylethylenediamine (TEMED) were from Bio-Rad Laboratories, U.S.A. EDTA, (disodium salt), silver nitrate, sodium carbonate and sodium nitrite were from Qualigens, India. All other reagents/chemicals were of the highest analytical grade available.
Ahmad S., Uddin M., Habib S., Shahab U., Alam K, & Ali A. (2014). Autoimmune response to AGE modified human DNA: Implications in type 1 diabetes mellitus. Journal of Clinical & Translational Endocrinology, 1(3), 66-72.
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6

Cytotoxicity Evaluation of Cell Lines

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Dulbecco's Modified Eagle Medium (DMEM) and Minimum Essential Medium (MEM), Dimethyl Sulphoxide (DMSO), Fetal Bovine Serum (FBS), Phosphate Buffer Saline (PBS), (3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT), Propidium Iodide (PI) and Triton X-100 were procured from Himedia (Mumbai, India). Rhodamine123 (Rh123) and 2,7-dichloroflourescin diacetate (DCFDA) were from Sigma Aldrich. 4′,6diamidino-2-phenylindole (DAPI) from Thermo fisher scientific. Primary antibodies, purified anti-caspase-3, PARP1 (F-2, sc-8007) and alpha-actinin were purchased from Biolegend and Santa Cruz Biotechnology, respectively. Lung adenocarcinoma (A549), cervical adenocarcinoma (HeLa) and prostate carcinoma (PC-3) cell lines were procured from the National Centre for Cell Sciences, Pune, India. The cells were cultured in DMEM medium containing 10% heat-inactivated FBS and penicillin/ streptomycin solution (100 units/ml and 100 mg/ml respectively) at 37°C in a humidified, 5% CO2 atmosphere.
Hira I., Kumari R., Saini A.K., Gullilat H., Saini V., Kumar A., & Saini R.V. (2021). Apoptotic Cell Death Induction Through Pectin, Guar Gum and Zinc Oxide Nanocomposite in A549 Lung Adenocarcinomas. Biointerface Research in Applied Chemistry, 12(2), 1856-1869.
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7

Evaluation of Anti-inflammatory Potential

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Triton X 100, amphotericin (5 mg/mL), and gentamycin (4 mg/mL) were purchased from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Fetal bovine serum (FBS), Pen Strep (a mixture of penicillin and streptomycin), and Dulbecco's modified Eagle medium (DMEM) were procured from Gibco Life Technologies (Bangalore, India); MTT (methylthiazolyl diphenyl-tetrazolium bromide), DMSO (dimethyl sulfoxide), Gallic acid, Quercetin, FeSO4·7H2O, F–C reagent (Folin Ciocalteu), 2,4,6-Tris (2-pyridyl)-1,3,5-triazine (TPTZ) were purchased from HiMedia Laboratories Pvt. Ltd. The cancer cell line MDA-MB-231 and HT1080 was obtained from National Centre for Cell Science (NCCS), Pune, Maharashtra, India. All other chemicals used were of analytical grade. UV–Visible spectrophotometer (SHIMADZU spectrophotometer UV-1800) was used for detection of absorbance. The anti-inflammatory activity was assessed using electrophoresis assembly (BioBee Tech, Bangalore) and gel documentation (Syngene, G Box, United Kingdom).
Kulkarni S.S., Mishra S., Patil S.B., Nambiar J, & Math A. (2021). Unifloral ajwain honey ameliorates differential inhibition of matrix metalloproteinases 2 and 9 protein, cytotoxicity, and antioxidant potential. Journal of Ayurveda and Integrative Medicine, 12(4), 633-639.
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8

Comprehensive Reagents and Cell Lines

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Unless otherwise mentioned all chemicals used are of the highest quality available and purchased from Sigma Aldrich (St. Louis, MO, USA). agarose, agar, ethidium bromide, catalase, methanol, ethanol, isopropanol, DMSO, NaCl, EDTA, Tris-HCl, Triton-X 100, NaOH, paraformaldehyde, bovien serum albumin (BSA), biotin, histidine, ampicillin, oxoid nutrient broth No. 2, and protease inhibitor cocktail were obtained from HiMedia (Mumbai, India), sodium fluoride and orthovandate from MP Biomedicals, LLC (Solon Ohio, USA), CellROX Deep Red and Nuc Blue (Hoechst 33342) from ThermoFisher Scientific (Santa Clara, CA), Salmonella typhimurium TA100 from Microbial Type Culture Collection and Gene Bank (MTCC, Chandigarh, India), thalidomide and DMEDA from Tokyo Chemical Industry (Tokyo, Japan), nitrocellulose membranes from MDI Membrane Technologies (Ambala. India), LMPA and LE agarose from Lonza (Rockland, ME, USA), γ-H2AX Alexa Fluor 488 from Biolegend (San Diego, CA, USA), and PAD-PARP antibody from Abcam (MA, USA). The 293T cell line was purchased from National Centre for Cell Culture (Pune, India) and authenticated by Short Tandem Repeat DNA profiling from Life Code Technology, Delhi, India. HepG2 cells were a generous gift from Dr. Soumya Sinha Roy at the CSIR Institute of Genomics and Integrative Biology, New Delhi. HUVEC was procured from HiMedia (Mumbai, India).
Wani T.H., Chakrabarty A., Shibata N., Yamazaki H., Guengerich F.P, & Chowdhury G. (2017). The Dihydroxy Metabolite of the Teratogen Thalidomide Causes Oxidative DNA Damage. Chemical research in toxicology, 30(8), 1622-1628.
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9

Synthesis of CIS Nanoparticles

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The synthesis of
CIS NPs is done using cupric chloride dihydrate (CuCl2·2H2O) [minimum assay 98%, Astron Chemicals, India], indium(III)
chloride tetrahydrate (InCl3·4H2O) [minimum
assay 99.99%, Sisco Research Laboratories (SRL) Pvt. Ltd., India],
thioacetamide (C2H5NS) [minimum assay 99%, Sisco
Research Laboratories (SRL) Pvt. Ltd., India], and Triton X-100 [minimum
hydroxyl value 85%, HiMedia Laboratories Pvt. Ltd., Mumbai, India].
All chemicals are used without any processing or purification.
Giri R.K., Chaki S., Khimani A.J., Vaidya Y.H., Thakor P., Thakkar A.B., Pandya S.J, & Deshpande M.P. (2021). Biocompatible CuInS2 Nanoparticles as Potential Antimicrobial, Antioxidant, and Cytotoxic Agents. ACS Omega, 6(40), 26533-26544.
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10

Formulation and Characterization of PLGA Nanoparticles

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PLGA (75:25), DSF and Pluronic 188® was purchased from Sigma Aldrich® (Bangalore, India). Polysorbate 80 and Triton X 100® were purchased from Himedia® (Mumbai, India). All other chemicals used were of analytical grade.
Hoda M., Sufi S.A., Cavuturu B, & Rajagopalan R. (2017). Stabilizers influence drug–polymer interactions and physicochemical properties of disulfiram-loaded poly-lactide-co-glycolide nanoparticles. Future Science OA, 4(2), FSO263.
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