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Pe rat igm

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PE rat IgM is a fluorescent-labeled antibody specific for the rat IgM immunoglobulin. It is a primary detection reagent used in flow cytometry and other immunoassays to identify and quantify IgM-expressing cells.

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Lab products found in correlation

Pe cy7 anti mouse cd24, by BioLegend (4 mentions) Percp cy5.5 rat igga, by BioLegend (4 mentions) Dnase 1, by Merck Group (3 mentions) Facsaria flow cytometer, by BD (3 mentions) Hyaluronidase, by Merck Group (3 mentions) Tryple, by Thermo Fisher Scientific (3 mentions) Collagenase, by Merck Group (3 mentions) Dispase, by Roche (3 mentions) Pe cy7 anti mouse epcam, by BioLegend (2 mentions) Alexa fluor 700, by BioLegend (2 mentions) Pe cy7 rat igg2a, by BioLegend (2 mentions) Apc cy7 rat igg2a, by BioLegend (2 mentions) Apc anti mouse cd45, by BioLegend (2 mentions) Apc anti mouse cd31, by BioLegend (2 mentions) Apc anti mouse ter119, by BioLegend (2 mentions) Biotin anti cd133, by BioLegend (2 mentions) Percp cy5.5 anti mouse sca 1, by BioLegend (2 mentions) Alexafluor700 anti mouse rat cd29, by BioLegend (2 mentions) Pe anti mouse cd49b, by BioLegend (2 mentions)

Spelling variants of this product

IgM-PE (9 citations)

4 protocols using pe rat igm

1

Single-cell Isolation and Flow Cytometry

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Single cell dissociation was performed through enzymatic digestion with 600 U ml-1 collagenase (Sigma) and 200 U ml-1 hyaluronidase (Sigma) for 90 min at 37 °C. Cells were further dissociated in TrypLE (Gibco) for 3 min, in 5 mg ml-1 dispase (Roche) and 0.1 mg ml-1 DNase I (Sigma) for 5 min, and then in 0.63% NH4Cl and filtered through a 40 μm cell strainer to obtain a single cell preparation for FACS. Cell labelling and flow cytometry were performed as described previously (Koren et al., 2015 (link)) using LSRII or FACS ARIA flow cytometers (BD). Dead cells (DAPI+), and CD45+/CD31+/Ter119+ (Lin+) non-epithelial cells were excluded. The following antibodies were all purchased from Biolegend and were used at a 1:100 final concentration: PE/Cy7 anti-mouse CD24, PE/Cy7 anti-mouse Epcam, AlexaFluor700 anti-mouse/rat CD29, APC/Cy7 anti-mouse CD49f, lineage markers: APC anti-mouse CD31, APC anti-mouse Ter119, APC anti-mouse CD45, isotype controls: PE rat IgM, PerCP/Cy5.5 rat IgGa, PE/Cy7 rat IgG2a, APC/Cy7 rat IgG2a, APC rat IgG2b. The purity of sorted populations was approximately 95%. The results were analyzed using FlowJo software (v10, BD).
Lloyd-Lewis B., Gobbo F., Perkins M., Jacquemin G., Huyghe M., Faraldo M.M, & Fre S. (2022). In vivo imaging of mammary epithelial cell dynamics in response to lineage-biased Wnt/β-catenin activation. Cell reports, 38(10), 110461.
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2

Multimarker Identification of Stem Cells

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Single cells were labeled with the required antibodies for 20 min at 4°C, washed, and re-suspended in DMEM/F12 medium containing 10 Mm HEPES, 5% FBS, and 1 mg/mL DAPI before analysis. The antibodies used were: biotin anti-CD133 (1:100; BioLegend), PE/Cy7 anti-mouse CD24 (1:100, BioLegend), PerCP/Cy5.5 anti-mouse Sca-1 (1:100, BioLegend), AlexaFluor700 anti-mouse/rat CD29 (1:100, BioLegend), PE anti-mouse CD49b (1:50, BioLegend); lineage markers: APC anti-mouse CD31 (1:100, BioLegend), APC anti-mouse Ter119 (1:100, BioLegend), APC anti-mouse CD45 (1:100, BioLegend); APC/Cy7 Streptavidin; isotype controls: PE rat IgM (1:50; BioLegend), PerCP/Cy5.5 rat IgGa (1:100, BioLegend). Single live cells were gated by DAPI exclusion and analyzed or sorted on LSRII, FACS Vantage or FACS Aria flow cytometers. The purity of sorted populations was about 95%.
Rodilla V., Dasti A., Huyghe M., Lafkas D., Laurent C., Reyal F, & Fre S. (2015). Luminal Progenitors Restrict Their Lineage Potential during Mammary Gland Development. PLoS Biology, 13(2), e1002069.
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3

Single-cell Isolation and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell dissociation was performed through enzymatic digestion with 600 U ml-1 collagenase (Sigma) and 200 U ml-1 hyaluronidase (Sigma) for 90 min at 37 °C. Cells were further dissociated in TrypLE (Gibco) for 3 min, in 5 mg ml-1 dispase (Roche) and 0.1 mg ml-1 DNase I (Sigma) for 5 min, and then in 0.63% NH4Cl and filtered through a 40 μm cell strainer to obtain a single cell preparation for FACS. Cell labelling and flow cytometry were performed as described previously (Koren et al., 2015 (link)) using LSRII or FACS ARIA flow cytometers (BD). Dead cells (DAPI+), and CD45+/CD31+/Ter119+ (Lin+) non-epithelial cells were excluded. The following antibodies were all purchased from Biolegend and were used at a 1:100 final concentration: PE/Cy7 anti-mouse CD24, PE/Cy7 anti-mouse Epcam, AlexaFluor700 anti-mouse/rat CD29, APC/Cy7 anti-mouse CD49f, lineage markers: APC anti-mouse CD31, APC anti-mouse Ter119, APC anti-mouse CD45, isotype controls: PE rat IgM, PerCP/Cy5.5 rat IgGa, PE/Cy7 rat IgG2a, APC/Cy7 rat IgG2a, APC rat IgG2b. The purity of sorted populations was approximately 95%. The results were analyzed using FlowJo software (v10, BD).
Lloyd-Lewis B., Gobbo F., Perkins M., Jacquemin G., Huyghe M., Faraldo M.M, & Fre S. (2022). In vivo imaging of mammary epithelial cell dynamics in response to lineage-biased Wnt/β-catenin activation. Cell reports, 38(10), 110461.
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4

Single-cell isolation and flow cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell dissociation was performed through enzymatic digestion with 600 U/mL collagenase (Sigma) and 200 U/mL hyaluronidase (Sigma) for 1 h at 37°C. Cells were further dissociated in TrypLE (Gibco) for 3 min, in 5 mg/mL dispase (Roche) and 0.1 mg/mL DNase I (Sigma) for 5 min, and then in 0.63% NH4Cl and filtered through a 40 μm cell strainer to obtain a single cell preparation for FACS. Cell labelling, and flow cytometry were performed as previously described in Rodilla et al., 2015. Dead cells (DAPI+), and CD45+/CD31+/Ter119+ (Lin+) non-epithelial cells were excluded before analysis using LSRII or FACS ARIA flow cytometers (BD). The following antibodies were used in 1:100 final concentration: biotin anti-CD133 (BioLegend), PE/Cy7 anti-mouse CD24 (BioLegend), PerCP/Cy5.5 anti-mouse Sca-1 (BioLegend), AlexaFluor700 anti-mouse/rat CD29 (BioLegend), PE anti-mouse CD49b (BioLegend); lineage markers: APC anti-mouse CD31 (BioLegend), APC anti-mouse Ter119 (BioLegend), APC anti-mouse CD45 (BioLegend); APC/Cy7 Streptavidin; isotype controls: PE rat IgM (BioLegend), PerCP/Cy5.5 rat IgGa (BioLegend). The purity of sorted populations was about 95%. The results were analyzed using FlowJo software and the data processing with Prism-graphpad.
Lilja A.M., Rodilla V., Huyghe M., Hannezo E., Landragin C., Renaud O., Leroy O., Rulands S., Simons B.D, & Fre S. (2018). Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain long-term plasticity in the embryonic mammary gland. Nature cell biology, 20(6), 677-687.
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