Cd64 apc h7

Manufactured by BD

Sourced in Germany

The CD64 APC-H7 is a laboratory instrument used for the detection and quantification of the CD64 antigen on the surface of cells. It utilizes flow cytometry technology to provide accurate and reliable measurements of CD64 expression.

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CD64-APC (10 citations)

3 protocols using cd64 apc h7

Multicolor Flow Cytometry Immunophenotyping

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Antibodies CD11b V450, CD16 PE-Cy7, CD18 PE, CD32PerCP-efluor 710, CD54 APC and CD64 APC-H7 were purchased from BD Bioscience (Heidelberg, Germany) and eBioscience (Frankfurt, Germany). As lineage markers, CD66b-FITC (granulocytes) from Beckman Coulter (Krefeld, Germany), CD3-eFluor 450 (T cells) from eBioscience, CD14-APC-Cy7 (monocytes) from BD Bioscience and CD56-BV510 (NK cells) from BioLegend (Fell, Germany) were used. Appropriate isotype antibodies from eBioscience, BD Bioscience and BioLegend were used as controls (see also S1 Table). Cells were incubated with antibodies for 30 min at 4°C and acquired on a BD FACS Canto II flow cytometer. Analysis and calculations were performed with BD FACS DIVA software.

Franklin C., Cesko E., Hillen U., Schilling B, & Brandau S. (2015). Modulation and Apoptosis of Neutrophil Granulocytes by Extracorporeal Photopheresis in the Treatment of Chronic Graft-Versus-Host Disease. PLoS ONE, 10(8), e0134518.

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Immune Phenotyping of Cryopreserved PBMCs

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Cryopreserved peripheral blood mononuclear cells (PBMCs) from COBRA participants and BBD were used for immune phenotyping. The PBMCs were thawed and subsequently stained with monoclonal antibodies (mAbs) for 30 minutes at 4°C in the dark, to determine expression of different surface molecules. The following directly conjugated mAbs were used for cell surface marker staining: CD14 PE-Cy7, CD16 eFluor 450, CD32 PerCP-eFluor 710, and CD11c APC (eBioscience, San Diego, CA); CD163 AlexaFluor 488 and CD86 PerCP (R&D Systems, Minneapolis, MN); CX3CR1 PerCP-Cy5.5 (BioLegend, San Diego, CA); HLA-DR V500, CD3 V500, CD4 PE-Cy7, HLA-DR fluorescein isothiocyanate (FITC), and CD38 PE (BD Biosiences, San Jose, CA); CD38 PE and CD91 PE (BD); and CD40 APC-H7, CD64 APC-H7, and CD8 Pacific Blue (BD Pharmingen, San Diego, CA). Fluorescence was measured with the FACS Canto II (BD Biosciences). The proportion of cells and the mean fluorescence intensity (MFI) of the markers was determined using FlowJo 7.6 (TreeStar, Ashland, OR).

Booiman T., Wit F.W., Maurer I., De Francesco D., Sabin C.A., Harskamp A.M., Prins M., Garagnani P., Pirazzini C., Franceschi C., Fuchs D., Gisslén M., Winston A., Reiss P, & Kootstra N.A. (2017). High Cellular Monocyte Activation in People Living With Human Immunodeficiency Virus on Combination Antiretroviral Therapy and Lifestyle-Matched Controls Is Associated With Greater Inflammation in Cerebrospinal Fluid. Open Forum Infectious Diseases, 4(3), ofx108.

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Immunohistochemistry and Flow Cytometry Analysis

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(i)

Immunohistochemistry. Immunohistochemical analysis was performed on 4 μm, formalin-fixed, paraffin-embedded sections in all cases. A broad immunohistochemical panel was utilized in the evaluation of the cases. Primary antibodies included CD34 (QBEnd/10, Leica (Novocastra)), CD117 (YR145, Cell Marque), CD71 (MRQ-48, Cell Marque), E-Cadherin (4A2C7, Life Technologies), Myeloperoxidase (Dako), Hemoglobin (Cell Marque), Glycophorin A (GA-R2, Ventana), CD61 and TP53 (DO-7, Ventana).

(ii)

Flow cytometry. After isotonic erythrocyte lysis, flow cytometric immunophenotyping was performed on anticoagulated bone marrow aspirate specimens using previously described methods [10 (link)]. Samples were examined with flow cytometric immunophenotyping using two eight-color tubes containing antibodies from BD Biosciences (Tube1: CD13 PE, CD15 V450, CD16 APC-H7, CD33 PE-Cy7, CD34 PerCP-Cy5.5, CD45 V500, CD117 APC, HLA-DR FITC and Tube2: CD2 FITC, CD7 PE, CD34 PerCP-Cy5.5, CD36 APC, CD38 V450, CD45 V500, CD56 PE-Cy7, CD64 APC-H7). A total of 100, 000 events were collected per case. The data were analyzed using Kaluza software (Beckman-Coulter, Brea, CA) and/or Diva software (BD Biosciences).

Reichard K.K., Tefferi A., Abdelmagid M., Orazi A., Alexandres C., Haack J, & Greipp P.T. (2022). Pure (acute) erythroid leukemia: morphology, immunophenotype, cytogenetics, mutations, treatment details, and survival data among 41 Mayo Clinic cases. Blood Cancer Journal, 12(11), 147.

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