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IRE1α is a core component of the unfolded protein response (UPR) pathway, a cellular stress response mechanism. It functions as a transmembrane protein that senses the accumulation of unfolded proteins in the endoplasmic reticulum (ER) and initiates a signaling cascade to restore ER homeostasis.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions) Phospho eif2α, by Abcam (2 mentions) Phospho ire1α, by Thermo Fisher Scientific (2 mentions) Phospho irf3, by Abcam (2 mentions) Sting, by Abcam (2 mentions) Tollip, by Abcam (2 mentions) Sting agonists, by InvivoGen (2 mentions) Mavs pathway agonist, by InvivoGen (2 mentions) Irak1, by Thermo Fisher Scientific (2 mentions) Hmgb1, by Abcam (2 mentions) Goat anti mouse igg, by Bio-Rad (2 mentions) Dmxaa, by InvivoGen (2 mentions) 2 3 cgamp, by InvivoGen (2 mentions) Goat anti rabbit igg, by Bio-Rad (2 mentions)

2 protocols using ire1α

1

ER Stress and Immune Signaling Pathways

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Herring testis DNA was used as dsDNA for immune stimulations (Sigma). 2’3’-cGAMP and DMXAA were used for STING agonists (Invivogen). PolyI:C was transfected as a MAVS-pathway agonist (Invivogen). Lipofectamine 2000 was used as transfection reagent for intracellular stimulations (Thermo Fisher).
Antibodies: ATF4 (CST D4B8), ATF6 (CST D4Z8V), BIP (CST C50B12), CHOP (CST L63F7), phospho-EIF2α (CST D968), HMGB1 (Abcam 18256), IRAK1 (CST D5167), IRE1α (CST 14C10), phospho-IRE1α (Thermo Fisher PA1-16927), IRF3 (CST D83B9), phospho-IRF3 (CST 4D4G), MAVS (CST 4983), STING (CST D2P2F), TOLLIP (Abcam ab187198), goat anti-rabbit IgG (Biorad 1706515), goat anti-mouse IgG (Biorad 1706516).
Pokatayev V., Yang K., Tu X., Dobbs N., Wu J., Kalb R.G, & Yan N. (2020). Homeostatic regulation of STING protein at the resting-state by stabilizer TOLLIP. Nature immunology, 21(2), 158-167.
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2

ER Stress and Immune Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Herring testis DNA was used as dsDNA for immune stimulations (Sigma). 2’3’-cGAMP and DMXAA were used for STING agonists (Invivogen). PolyI:C was transfected as a MAVS-pathway agonist (Invivogen). Lipofectamine 2000 was used as transfection reagent for intracellular stimulations (Thermo Fisher).
Antibodies: ATF4 (CST D4B8), ATF6 (CST D4Z8V), BIP (CST C50B12), CHOP (CST L63F7), phospho-EIF2α (CST D968), HMGB1 (Abcam 18256), IRAK1 (CST D5167), IRE1α (CST 14C10), phospho-IRE1α (Thermo Fisher PA1-16927), IRF3 (CST D83B9), phospho-IRF3 (CST 4D4G), MAVS (CST 4983), STING (CST D2P2F), TOLLIP (Abcam ab187198), goat anti-rabbit IgG (Biorad 1706515), goat anti-mouse IgG (Biorad 1706516).
Pokatayev V., Yang K., Tu X., Dobbs N., Wu J., Kalb R.G, & Yan N. (2020). Homeostatic regulation of STING protein at the resting-state by stabilizer TOLLIP. Nature immunology, 21(2), 158-167.
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