Phosphatase inhibitor cocktail 2 p5726

Immediately following the final LMA test session, the mice were euthanized by cervical dislocation, and brains were removed and stored at -80°C until microdissection to obtain striatal tissue samples. Trunk blood samples from the BEC-Test cohort were also collected and centrifuged at 5,000 rpm for 10 min to obtain plasma, which was stored at -80°C until use. Brains from the Immuno-Home cohort were sliced into 2 mm sections using a rodent brain slicer matrix (Zivic Instruments, Pittsburgh, PA), and unilateral 1.5 mm tissue punches (Miltex, Inc., York, PA) from the left anterior-dorsal striatum at 1.5 to -0.5 mm anterior to bregma were taken and stored at -80°C until immunoassay. A unilateral punch was then homogenized for approximately 20 seconds in lysis buffer (20 mM Tris pH 7.5, 150 mM NaCl, 2mM EDTA, 1% triton X-100, 10% glycerol with Phosphatase Inhibitor Cocktail 2 P5726, Phosphatase Inhibitor Cocktail 3 P0044 and Protease Inhibitor P8340 from Sigma Aldrich, St. Louis, MO), then centrifuged at 4°C for 10 min at 10,000 x g, and the protein was quantified using a Pierce 660 nm protein assay using a bovine serum albumin standard (Thermo Scientific, Rockford, IL).