Alexafluor488 and cy3 labeled secondary antibodies

Manufactured by Jackson ImmunoResearch

Sourced in Cameroon

AlexaFluor488- and Cy3-labeled secondary antibodies are fluorescently-conjugated secondary antibodies used in various immunoassay techniques. These antibodies specifically bind to the primary antibodies, allowing for the detection and visualization of target molecules or proteins.

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Lab products found in correlation

Chelex 100 resin, by Merck Group (4 mentions) Chelex 100 resin 50 100 mesh, by Merck Group (3 mentions) Dodium citrate dehydrate, by Merck Group (2 mentions) Absolute ethanol, by Thermo Fisher Scientific (2 mentions) Hematoxylin staining solution, by Merck Group (2 mentions) Scm peg5k mal, by Creative PEGWorks (2 mentions) Pd 10 column, by GE Healthcare (2 mentions) Nhs fluorescein, by Merck Group (2 mentions) P scn bn nota, by Macrocyclics (2 mentions) Pd 10 desalting column, by GE Healthcare (1 mentions) Rat anti mouse cd31 primary antibody, by BD (1 mentions) Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions) Petrace cyclotron, by GE Healthcare (1 mentions) S 2 4 isothiocyanatobenzyl 1 4 7 triazacyclononane 1 4 7 triacetic acid p scn bn nota, by Macrocyclics (1 mentions) Dapi nuclear counterstain, by Thermo Fisher Scientific (1 mentions) G3bp1, by Proteintech (1 mentions)

5 protocols using alexafluor488 and cy3 labeled secondary antibodies

Synthesis and Characterization of Functionalized Nanoparticles

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TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). p-SCN-Bn-NOTA (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, hematoxylin staining solution, and Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-Aminopropyl)triethoxysilane (APS), Cetyltrimethylammonium chloride solution (CTAC, 25 wt %), copper(II) chloride (CuCl₂), sodium sulfide nonahydrate (Na₂S·9H₂O) and dodium citrate dehydrate were purchased from Sigma-Aldrich (St. Louis, MO). AlexaFluor488- and Cy3-labeled secondary antibodies were purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, sodium chloride (NaCl), doxorubicin hydrochloride (DOX) were purchased from Fisher Scientific. SCM-PEG_5k-Mal was obtained from Creative PEGworks. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.

Chen F., Hong H., Goel S., Graves S.A., Orbay H., Ehlerding E.B., Shi S., Theuer C.P., Nickles R.J, & Cai W. (2015). In Vivo Tumor Vasculature Targeting of CuS@MSN Based Theranostic Nanomedicine. ACS Nano, 9(4), 3926-3934.

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Radiolabeling of CD31 Antibody with Deferoxamine

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ALT-836 was kindly supplied by Altor Bioscience Corp. 1-(4-isothiocyanatophenyl)-3-[6,17-dihydroxy-7,10,18,21-tetraoxo-27-(N-acetylhydroxylamino)- 6,11,17, 22- tetraazaheptaeicosine] thiourea (p-SCN -Bn-Deferoxamine or Df) was purchased form Macrocyclics, Incorporation (Plano, TX) and Chelex 100 resin (50–100 mesh) was acquired from Sigma Aldrich (St. Louis, MO). Primary rat anti-mouse CD31 mAb was purchased for Novus Biologicals (Littleton, CO) and Alexafluor488 and Cy3-labeled secondary antibodies were procured by Jackson ImmunoResearch Laboratories (West Grove, PA). Milli-Q water (resistivity > 18.2 MΩ•cm) was employed in the preparation of all buffers and solutions. Buffers used for radiochemistry were treated with Chelex 100. The rest of the materials and reagents were purchase from Thermo Fisher Scientific Incorporation (Waltham, MA).

Hernandez R., England C.G., Yang Y., Valdovinos H.F., Liu B., Wong H.C., Barnhart T.E, & Cai W. (2017). ImmunoPET imaging of tissue factor expression in pancreatic cancer with 89Zr-Df-ALT-836. Journal of controlled release : official journal of the Controlled Release Society, 264, 160-168.

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Radiolabeling and Imaging of CD31

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ALT-836 was provided by Altor Bioscience Corporation (Miramar, FL). S-2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA) was purchased from Macrocyclics, Inc. (Dallas, TX). Chelex 100 resin (50-100 mesh) and fluorescein isothiocyanate (FITC) were acquired from Sigma-Aldrich (St. Louis, MO). Rat anti-mouse CD31 primary antibody was purchased from BD Biosciences (San Diego, CA). AlexaFluor488- and Cy₃-labeled secondary antibodies were purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). PD-10 desalting columns were purchased from GE Healthcare (Piscataway, NJ). ⁶⁴Cu was produced by a GE PETrace cyclotron using the ⁶⁴Ni(p,n)⁶⁴Cu reaction, which has specific activity of > 5 Ci/μmol at the end of bombardment. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All other reaction buffers and chemicals were from Thermo Fisher Scientific.

Shi S., Hong H., Orbay H., Graves S.A., Yang Y., Ohman J.D., Liu B., Nickles R.J., Wong H.C, & Cai W. (2015). ImmunoPET of Tissue Factor Expression in Triple-Negative Breast Cancer with a Radiolabeled Antibody Fab Fragment. European journal of nuclear medicine and molecular imaging, 42(8), 1295-1303.

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Bioconjugation of TRC105 with NOTA

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TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). p-SCN-Bn-NOTA (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7- triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, hematoxylin staining solution, and Chelex 100 resin (50~100 mesh), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-Aminopropyl)triethoxysilane (APS), Cetyltrimethylammonium chloride solution (CTAC, 25 wt%), copper(II) chloride (CuCl₂), sodium sulfide nonahydrate (Na₂S·9H₂O) and dodium citrate dehydrate were purchased from Sigma-Aldrich (St. Louis, MO). AlexaFluor488- and Cy3-labeled secondary antibodies were purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, sodium chloride (NaCl), doxorubicin hydrochloride (DOX) were purchased from Fisher Scientific. SCM-PEG_5k-Mal was obtained from Creative PEGworks. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.

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Immunofluorescence Analysis of ALS/FTLD Pathology

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Formalin-fixed, paraffin-embedded human hippocampus and cervical spinal cord sections of ALS/FTLD subjects retrieved from the Neurodegenerative Brain Bank at the University of Pittsburgh, following protocols approved by the University of Pittsburgh Committee for Oversight of Research and Clinical Training Involving Decedents (CORID). Following antigen retrieval with Target Retrieval Solution, pH 9 (Dako Agilent), immunofluorescence staining was performed using the following primary antibodies: TIA1 (1:1000, Abeam), Ataxin 2 (1:50, Proteintech), G3BP1 (1:100, Proteintech) and pTDP-43 (1D3, 1:500, kindly provided by Manuela Neumann, Helmholtz Zentrum, Munich, Germany). Immunofluorescence signal was visualized using Alexa Fluor 488 and Cy3 labeled secondary antibodies (both 1:200, Jackson ImmunoResearch) and DAPI nuclear counterstain (1:1000, Thermo Scientific).

Mann J.R., Gleixner A.M., Mauna J.C., Gomes E., DeChellis-Marks M.R., Needham P.G., Copley K.E., Hurtle B., Portz B., Pyles N.J., Guo L., Calder C.B., Wills Z.P., Pandey U.B., Kofler J.K., Brodsky J.L., Thathiah A., Shorter J, & Donnelly C.J. (2019). RNA binding antagonizes neurotoxic phase transitions of TDP-43.. Neuron, 102(2), 321-338.e8.

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