HEK293T, Vero, Huh7, and BHK-21 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies), supplemented with 10% by volume heat inactivated Fetal Bovine Serum (FBS, Life Technologies), and 1% by volume Penicillin-Streptomycin-Glutamine (PSG, Life Technologies).
Drosophila Schneider S2 cells were grown in Schneider’s Drosophila Medium at 27°C to generate stable cell lines. Insect-XPRESS protein-free medium with L-glutamine (Lonza) supplemented with puromycin (6 μg/mL) (InvivoGen) was used to grow S2 cells at 27°C for protein expression.
Drosophila Schneider S2 cells were grown in Schneider’s Drosophila Medium at 27°C to generate stable cell lines. Insect-XPRESS protein-free medium with L-glutamine (Lonza) supplemented with puromycin (6 μg/mL) (InvivoGen) was used to grow S2 cells at 27°C for protein expression.