5-Fluorodeoxyuridine was purchased from Alfa Aesar (Shanghai, China). 4,4ʹ-Dimethoxytrityl Chloride (DMT-Cl, 97%) N,N-Diisopropylethylamine (DIPEA, 97%) and 2-Cyanoethyl N,N,N’,N’-tetraisopropylphosphordiamidite (95%) were obtained from Macklin Inc. (Shanghai, China). N-(ε-malemidocaproyloxy) succinimide ester (EMCS) was purchased from Aladdin (Shanghai, China). Ni-NTA agarose, and Sephadex G-25 were obtained from GE Healthcare (Piscataway, USA). Imidazole, sodium chloride, sodium acetate, polyacrylamide, tris base, acetic acid, ethylenediaminetetraacetic acid (EDTA), magnesium chloride, ethanol, and DAPI were obtained from Sangon Biotech (Shanghai) Co., Ltd (China). Amicon ultracentrifugal filters were purchased from Merck Millipore Ltd (Darmstadt, Germany). RPMI 1640 medium, DMEM medium, MEBM medium, trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), antibiotic-antimycotic (100×) and fetal bovine serum (FBS) were purchased from Wisent Biotechnology (Nanjing) Co. Ltd (China). All chemicals were used without further purification.
Magnesium chloride
Manufactured by Sangon
Sourced in China
Magnesium chloride is a chemical compound with the formula MgCl2. It is a colorless, crystalline solid that is highly soluble in water. Magnesium chloride is commonly used as a source of magnesium ions in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Sangon (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Sangon (2 mentions)
Sephadex g 25, by GE Healthcare (2 mentions)
Amicon ultra centrifugal filter, by Merck Group (2 mentions)
Sodium chloride (nacl), by Sangon (2 mentions)
Imidazole, by Sangon (2 mentions)
Sodium acetate, by Sangon (2 mentions)
Ni nta agarose, by GE Healthcare (2 mentions)
Acetic acid, by Sangon (2 mentions)
Maltose, by Merck Group (2 mentions)
Sorbitol, by Merck Group (2 mentions)
Soluble starch, by Merck Group (2 mentions)
Sucrose, by Merck Group (2 mentions)
Fructose, by Merck Group (2 mentions)
Mannitol, by Merck Group (2 mentions)
Lactose, by Merck Group (2 mentions)
Copper chloride, by Sangon (2 mentions)
Bagasse, by Merck Group (2 mentions)
Manganese chloride, by Sangon (2 mentions)
Cobalt chloride, by Sangon (2 mentions)
5 protocols using magnesium chloride
1
Reagents for Biomolecular Modification
2
Isolation and Characterization of GXDK6 from Mangrove Sediments
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GXDK6 was isolated from the subtropical mangrove sediments in Beibu Gulf of South China Sea (21°29′25.74″N, 109°45′49.43″E) and deposited in the China General Microbiological Culture Collection Center (CGMCC) under CGMCC No. 16007.
The chemical reagents were analytical grade. Glucose, fructose, maltose, sorbitol, lactose, sucrose, mannitol, soluble starch, bran, cassava flour, and bagasse were purchased from Sigma-Aldrich, Inc. (Darmstadt, Germany). Yeast powder, agar powder, peptone, HCl, NaOH, NaCl, and KCl were purchased from Novagen (Darmstadt, Germany). Magnesium chloride, copper chloride, manganese chloride, and cobalt chloride were purchased from Sangon Biotech (Shanghai) Co., Ltd., (Shanghai, China).
The chemical reagents were analytical grade. Glucose, fructose, maltose, sorbitol, lactose, sucrose, mannitol, soluble starch, bran, cassava flour, and bagasse were purchased from Sigma-Aldrich, Inc. (Darmstadt, Germany). Yeast powder, agar powder, peptone, HCl, NaOH, NaCl, and KCl were purchased from Novagen (Darmstadt, Germany). Magnesium chloride, copper chloride, manganese chloride, and cobalt chloride were purchased from Sangon Biotech (Shanghai) Co., Ltd., (Shanghai, China).
3
Synthesis and Characterization of EMCS-Pt Conjugate
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The DNAs were synthesized from Sangon Biotech (Shanghai) Co., Ltd. N-(ε-maleimidocaproyloxy)succinimide ester (EMCS) and cis-platinum was purchased from Aladdin (Shanghai, China). Ni-NTA agarose and Sephadex G-25 were obtained from GE Healthcare (Piscataway, NJ, USA). Imidazole, sodium chloride, sodium acetate, polyacrylamide, trizma base, acetic acid, ethylenediaminetetraacetic acid (EDTA), magnesium chloride, ethanol, sodium diethyldithiocarbamate (DDTC) and DAPI were obtained from Sangon Biotech (Shanghai) Co., Ltd. Amicon® ultracentrifugal filters were purchased from Merck Millipore Ltd. (Darmstadt, Germany). Gibco® RPMI 1640 medium, trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), dimethylsulfoxide (DMSO), antibiotic–antimycotic (100×), fetal bovine serum (FBS), and DAPI were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All chemicals were purchased and used without further purification.
4
Cellular Endocytosis Pathway Inhibitors
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Hoechst 33342 purchased from Beyotime (Shanghai, China). β-Galactosidase, potassium ferricyanide, potassium ferrocyanide, magnesium chloride and X-gal were obtained from Sangon Biotech (Shanghai, China). Chlorpromazine (CPZ, a clathrin-mediated endocytosis inhibitor [9 (link)], methylated-β-cyclodextrin (MβCD, a caveolae-mediated endocytosis inhibitor, a cholesterol depletion agent [10 (link)]), 5-(N-ethyl-N-isopropyl) amiloride (EIPA, a macropinocytosis inhibitor, selectively blocks the Na+/H+ antiporter [11 (link)]) and LY294002 (a macropinocytosis inhibitor, a strong phosphatidylinositol-3 kinase inhibitor [12 (link)]) were obtained from Aladdin Industrial (Shanghai, China).
5
Isolation and Characterization of GXDK6
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GXDK6 was isolated from the subtropical mangrove sediments in the Beibu Gulf of South China Sea (21°29′25.74″N, 109°45′49.43″E) and deposited in the China General Microbiological Culture Collection Center (CGMCC) under CGMCC No. 16007.
The chemical reagents were of analytical grade. Glucose, fructose, maltose, sorbitol, lactose, sucrose, mannitol, soluble starch, bran, cassava our, and bagasse were purchased from Sigma-Aldrich, Inc. (Darmstadt, Germany). Yeast powder, agar powder, peptone, HCl, NaOH, NaCl, and KCl were purchased from Novagen (Darmstadt, Germany). Magnesium chloride, copper chloride, manganese chloride, and cobalt chloride were purchased from Sangon Biotech (Shanghai) Co., Ltd., (Shanghai, China).
The chemical reagents were of analytical grade. Glucose, fructose, maltose, sorbitol, lactose, sucrose, mannitol, soluble starch, bran, cassava our, and bagasse were purchased from Sigma-Aldrich, Inc. (Darmstadt, Germany). Yeast powder, agar powder, peptone, HCl, NaOH, NaCl, and KCl were purchased from Novagen (Darmstadt, Germany). Magnesium chloride, copper chloride, manganese chloride, and cobalt chloride were purchased from Sangon Biotech (Shanghai) Co., Ltd., (Shanghai, China).
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