Log In Sign up
The largest database of trusted experimental protocols

Ab194697

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

Ab194697 is a primary antibody product offered by Abcam. It is a recombinant monoclonal antibody derived from human tissue. The antibody is designed to detect a specific target protein.

Automatically generated - may contain errors

Lab products found in correlation

Ab32063, by Abcam (1 mentions) Ab170904, by Abcam (1 mentions) Ym3029, by Immunoway (1 mentions) Df6433, by Affinity Biosciences (1 mentions) Sc 5770, by Santa Cruz Biotechnology (1 mentions) Polyvinylidene fluoride pvdf membrane, by Merck Group (1 mentions) Ab194113, by Abcam (1 mentions) Ecl chemiluminescence system, by Merck Group (1 mentions) Bca assay kit, by Thermo Fisher Scientific (1 mentions) Ab3568, by Abcam (1 mentions) Hrp conjugated anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Ab33586, by Abcam (1 mentions) Nitrocellulose membrane, by Pall Corporation (1 mentions) β actin, by Proteintech (1 mentions)

5 protocols using ab194697

1

Western Blot and Co-Immunoprecipitation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The proteins were dealt with SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) gel, then transferring of membranes was performed. Moreover, membranes were blocked with skim milk and probed using the primary antibody. The following antibodies were used in this study: β-actin (Abcam; ab194697: WB 1:2000), UGT1A1 (Abcam; ab194697: WB 1:2000; IF 1:400), and anti-Estrogen Receptor alpha (ERα) antibody (Abcam; ab32063: WB 1:1000; IF 1:200), respectively. Then the chemiluminescence-based detection was conducted according to the secondary antibody conjugated horseradish peroxidase with exposed film. The assays of co-immunoprecipitation (CO-IP) were immunoprecipitated with Suspension Agarose beads (Protein G Plus/Protein A).
Li Y., Zhou Y., Mao F., Shen S., Zhao B., Xu Y., Lin Y., Zhang X., Cao X., Xu Y., Chen C., Zhang J, & Sun Q. (2020). miR-452 Reverses Abnormal Glycosylation Modification of ERα and Estrogen Resistance in TNBC (Triple-Negative Breast Cancer) Through Targeting UGT1A1. Frontiers in Oncology, 10, 1509.
+ Open protocol
+ Expand
2

Quantitative Analysis of Hepatic and Intestinal Transporters

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The livers and jejunum tissue were homogenized in ice-cold radio immunoprecipitation assay (RIPA) buffer [50 mM Tris HCl, 1 mM EDTA, 150 mM NaCl, 0.1% sodium dodecyl sulfate, 0.5% sodium deoxycholate, 1.0% Nonidet P-40, and phenylmethane sulfonyl fluoride (PMSF), pH 8.0]. The homogenate was centrifuged at 12,000 rpm for 20 min at 4°C. The supernatants were collected for Western blot analysis, which was performed as described previously (Kong et al., 2015 (link)). The antibodies used in the present study included anti-Mrp-2 (1:500; sc-5770, Santa Cruz; California, USA), anti-glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) (1:20,000; YM3029, Immunoway; Texas, USA), anti-UGT1A1 (1:4,000; ab194697, Abcam; Cambs, UK), anti-P glycoprotein (1:1,000; ab170904, Abcam; Cambs, UK), anti-CES2 (1:1,000; DF6433, Affinity; Cambs, UK), and anti-Bcrp (1:200; sc-69988, Santa Cruz; California, USA). The gray intensities of the bands were quantified using ImageJ software (National Institute of Health, MD, USA).
Guan H.Y., Li P.F., Wang X.M., Yue J.J., He Y., Luo X.M., Su M.F., Liao S.G, & Shi Y. (2017). Shengjiang Xiexin Decoction Alters Pharmacokinetics of Irinotecan by Regulating Metabolic Enzymes and Transporters: A Multi-Target Therapy for Alleviating the Gastrointestinal Toxicity. Frontiers in Pharmacology, 8, 769.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of UGT1A1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were fixed with neutral formalin (10%), then dehydrated and embedded with paraffin. Moreover, sections (5 μm) were cut from these tissues; primary antibody of UGT1A1 (Abcam, ab194697, 1:200) and the matched secondary antibody were further used to incubate after antigen retrieval, respectively. Besides, after staining with DAB substrate kit (Pierce, USA), these sections were observed under Olympus microscope to obtain a photograph of the expression status, including subcellular location, expression level, and expression density.
Li Y., Zhou Y., Mao F., Shen S., Zhao B., Xu Y., Lin Y., Zhang X., Cao X., Xu Y., Chen C., Zhang J, & Sun Q. (2020). miR-452 Reverses Abnormal Glycosylation Modification of ERα and Estrogen Resistance in TNBC (Triple-Negative Breast Cancer) Through Targeting UGT1A1. Frontiers in Oncology, 10, 1509.
+ Open protocol
+ Expand
4

Western Blot Analysis of Hepatic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extracted from liver tissue were obtained using Radio Immunoprecipitation Assay (RIPA) lysis buffer (1 mM phenylmethanesulfonyl fluoride, PMSF) according to the manufacturer’s instruction (Beyotime Biotechnology Co. Ltd, China). Protein concentration were measured by the BCA assay kit (ThermoFisher Scientific, Allentown PA). Forty µg of each lysate sample was boiled for 10 min, separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, USA). Nonspecific reactivity was blocked in 5% nonfat dry milk in Tris-buffered saline (0.05% Tween-20) (TBST) for 1h at room temperature. The membranes were incubated overnight with primary antibodies including anti-SULT2A1 (ab194113, Abcam, Cambridge, MA), anti-UGT1A1 (ab194697, Abcam, Cambridge, MA) and β-actin (Jinqiao Technology Co. Ltd, China) followed by reaction with a secondary horseradish peroxidase-conjugated (HRP) anti-rabbit IgG antibody (Jinqiao Technology Co. Ltd, China). Specific bands were detected by use of the ECL Chemiluminescence System (Merck Millipore, USA).
Wang H., Fang Z.Z., Meng R., Cao Y.F., Tanaka N., Krausz K.W, & Gonzalez F.J. (2017). Glycyrrhizin and glycyrrhetinic acid inhibits alpha-naphthyl isothiocyanate-induced liver injury and bile acid cycle disruption. Toxicology, 386, 133-142.
+ Open protocol
+ Expand
5

Liver Protein Extraction and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cellular protein of liver tissues was extracted by RIPA lysis buffer (150 M of NaCl, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, and 50 mM Tris (pH 7.4)) containing protease inhibitors. 20 μg soluble proteins were separated by 8 ~ 12% SDS-PAGE and were transferred onto a nitrocellulose membrane (Pall Corporation, NY, USA). After being blocked with 5% fat free milk, the membranes were incubated with primary antibodies against caspase3 (#9662), cleaved caspase 3 (#9664), CYP1A2 (#14719) (Cell Signaling Technology, MA, USA), CYP1A1 (#ab3568), CYP1B1 (#ab33586), GSTT1 (#ab199337), GSTM1 (#ab113432), UGT1A1 (#ab194697) (Abcam, Cambridge, UK), and β-actin (#60008; Proteintech Group, AL, USA). Immunolabeling was visualized with HRP-conjugated anti-rabbit IgG or anti-mouse IgG (Santa Cruz Biotechnology, CA, USA). The density of the specific bands was quantified using ImageJ software.
Li D., Chen S., Li Q., Chen L., Zhang H., Li H., Yu D., Zhang R., Niu Y., Lu S., Ye L., Zeng X., Dong G., Chen R., Aschner M., Zheng Y, & Chen W. (2020). Caloric restriction attenuates C57BL/6 J mouse lung injury and extra-pulmonary toxicity induced by real ambient particulate matter exposure. Particle and Fibre Toxicology, 17, 22.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!