Anti galectin 3

Whole cell lysates were obtained by re-suspending cell pellets in RIPA buffer with freshly added protease inhibitor tablet (Roche). Immunoprecipitation was performed essentially as previously described (Yang et al., 2018 (link)). Briefly, anti-Brg1 (Santa Cruz, sc-17796), or pre-immune IgGs (P.I.I.) were added to and incubated with cell lysates overnight before being absorbed by Protein A/G-plus Agarose beads (Santa Cruz). Precipitated immune complex was released by boiling with 1X SDS electrophoresis sample buffer. Western analyses were performed with anti-β-actin (Sigma), anti-Brg1 (Santa Cruz, sc-17796), anti-TET1 (Active Motif, 61443), anti-c-Jun (Santa Cruz, sc-1694), anti-c-Fos (Santa Cruz, sc-52), or anti-Galectin-3 (Proteintech, 14979-1). Uncropped full blots are included in the Supplementary Material (Supplementary Figures S1–S4).

Formaldehyde- or paraformaldehyde-fixed tumor tissues were embedded in paraffin, and sections were stained with H&E using standard techniques. The following primary antibodies were used: anti-FLAG M2 (Sigma-Aldrich), anti-Sox9 (Sigma-Aldrich), anti-Hey1 (Abcam), anti-Runx2 (MBL), anti-CK18 (Proteintech), anti-Galectin 3 (Proteintech), anti-Ki-67 (Abcam), and anti-NCOA2/SRC2 (Cell Signaling).
Immunoblotting was performed using whole-cell lysates. The following primary antibodies were used: anti-FLAG M2 (Sigma-Aldrich), anti-Runx2 (MBL), anti-Runx3 (Abcam), anti–α-tubulin (Sigma-Aldrich), and anti-Cas9 (Novus, Centennial, CO).