Hp1022

Manufactured by Valley Biomedical

The HP1022 is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 15,000 RPM and a maximum relative centrifugal force of 21,382 g. The centrifuge accommodates rotor sizes up to 6 x 50 mL.

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Lab products found in correlation

D16 1, by Thermo Fisher Scientific (2 mentions) Bp3101, by Merck Group (2 mentions) Sx03201, by Thermo Fisher Scientific (2 mentions) Ss266 1, by Thermo Fisher Scientific (2 mentions) A11481 06, by Corning (2 mentions) Cd14 microbead, by Miltenyi Biotec (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Ascorbic acid, by Thermo Fisher Scientific (1 mentions) Human il 2, by Thermo Fisher Scientific (1 mentions) Ethanolamine, by Merck Group (1 mentions) Sodium selenite, by Merck Group (1 mentions) E0135, by Merck Group (1 mentions) X vivo 15, by Lonza (1 mentions) Neutralized n acetyl l cysteine, by Merck Group (1 mentions)

4 protocols using hp1022

Plasmodium falciparum 3D7 Culture Protocol

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P. falciparum 3D7 parasites were cultured as previously described (101 (link)). In short, parasites were cultured in human AB⁺ erythrocytes (Interstate Blood Bank, Memphis, TN, USA) at 3%–10% parasitemia in a complete culture medium (5% hematocrit). Complete culture medium consisted of RPMI 1,640 medium (Gibco #32404014) supplemented with gentamicin (45 µg/mL final concentration; Gibco #15710064), HEPES (40 mM; Fisher #BP3101), NaHCO₃ (1.9 mg/mL; Sigma #SX03201), NaOH (2.7 mM; Fisher #SS266-1), hypoxanthine (17 µg/mL; Alfa Aesar #A11481-06), L-glutamine (2.1 mM; Corning #25005 Cl), D-glucose (2.1 mg/mL; Fisher #D16-1), and 10% (vol/vol) human AB⁺ serum (Valley Biomedical #HP1022). Parasites were cultured at 37°C in an atmosphere of 5% O₂, 5% CO₂, and 90% N₂.

McLellan J.L., Sausman W., Reers A.B., Bunnik E.M, & Hanson K.K. (2023). Single-cell quantitative bioimaging of P. berghei liver stage translation. mSphere, 8(6), e00544-23.

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Isolation and Culture of Human NK Cells

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Human subjects were used as per the Institutional Review Board approval and with their consent. Adult peripheral blood was obtained from healthy donors. PBMCs were isolated by centrifugation using a Ficoll–Paque Premium. NK cells were negatively selected from PBMCs using Clini MACS CD3 Reagent (273-01, Miltenyi Biotec), and cultured at 37°C with 5% CO₂ in a 3.5:1 (v:v) mix of DMEM (11995; Life Technologies) and F-12 (11765; Life Technologies) containing 10% human heat-inactive serum (HP1022; Valley Biomedical), 25 µM 2-ME (21985-023; Invitrogen), 50 µM ethanolamine (E0135; Sigma), 1.7 µg/l sodium selenite (214485; Sigma), 20 mg/l ascorbic acid, and 1000 U/ml human IL-2 (200-02; Peprotech). Monocytes were isolated by positive magnetic selection using CD14 microbeads (Miltenyi Biotech) according to the manufacturer’s instructions. B, T, and NK cells were all isolated by negative magnetic bead selection according to the manufacturer’s instructions.

Iizuka Y., Cichocki F., Sieben A., Sforza F., Karim R., Coughlin K., Vogel R.I., Gavioli R., McCullar V., Lenvik T., Lee M., Miller J, & Bazzaro M. (2015). UNC-45A Is a Nonmuscle Myosin IIA Chaperone Required for NK Cell Cytotoxicity via Control of Lytic Granule Secretion. Journal of immunology (Baltimore, Md. : 1950), 195(10), 4760-4770.

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Culturing P. falciparum 3D7 Parasites

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P. falciparum 3D7 parasites were cultured as previously described (101 (link)). In short, parasites were cultured in human AB⁺ erythrocytes (Interstate Blood Bank, Memphis, TN, USA) at 3 – 10% parasitemia in complete culture medium (5% hematocrit). Complete culture medium consisted of RPMI 1640 medium (Gibco #32404014) supplemented with gentamicin (45 µg/ml final concentration; Gibco #15710064), HEPES (40 mM; Fisher #BP3101), NaHCO₃ (1.9 mg/ml; Sigma #SX03201), NaOH (2.7 mM; Fisher #SS266-1), hypoxanthine (17 µg/ml; Alfa Aesar #A11481-;06), L-glutamine (2.1 mM; Corning #25005Cl), D-glucose (2.1 mg/ml; Fisher #D16-1), and 10% (vol/vol) human AB⁺ serum (Valley Biomedical #HP1022). Parasites were cultured at 37°C in an atmosphere of 5% O₂, 5% CO₂, and 90% N₂.

McLellan J.L., Sausman W., Reers A.B., Bunnik E.M, & Hanson K.K. (2023). Single-cell quantitative bioimaging of P. berghei liver stage translation. bioRxiv.

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Isolation and Culture of Primary T Cells

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Primary CD4+ and CD8+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 & 15063). Blood was obtained from Blood Centers of the Pacific (San Francisco, CA) as approved by the University Institutional Review Board. T cells were cryopreserved in RPMI-1640 (UCSF cell culture core) with 20% human AB serum (Valley Biomedical Inc., #HP1022) and 10% DMSO. After thawing, T cells were cultured in human T cell medium consisting of X-VIVO 15 (Lonza #04-418Q), 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository) for all experiments.

Roybal K.T., Rupp L.J., Morsut L., Walker W.J., McNally K.A., Park J.S, & Lim W.A. (2016). Precision Tumor Recognition by T Cells With Combinatorial Antigen Sensing Circuits. Cell, 164(4), 770-779.

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