Prfp c rs scrambled shrna

β₁-integrin antibody (AIIB2) was obtained from the Developmental Studies Hybridoma Bank at the University of Iowa (1:200); antibodies to β₁-integrin-12G10 (1:200 IF, 1:1000 WB), CD9 (1:1000 WB, 1:50 IP) and HE4 (1:600) were purchased from Abcam; MT1-MMP antibody was from Millipore (1:100 IF, 1:1000 WB); anti-RFP antibody was from Rockland Immunochemicals (1:1200); α-tubulin antibody was obtained from Sigma (1:1000); antibodies to BAP31 (1:1000), VAMP3 (1:750), and CA-125 (1:1000) were purchased from Pierce (Rockford, IL.); MHC-I antibody was a gift from Dr. Janice Blum (IUPUI, Indianapolis, IN.); antibodies to ARF6 were described previously ^{48 (link)}. FITC-phalloidin, AF647-phalloidin, TexasRed and Cy5 secondary antibodies were from Jackson ImmunoResearch (1:300); HRP conjugated secondary antibodies were purchased from Cell Signaling (1:5000). GFP-VAMP3 and GFP-VAMP7 were a gift from T. Galli (Institut Jacques Monod, Paris). MT1-MMP-mCherry was a gift from P. Chavrier (Institut Curie, Centre de Recherche, Paris). pRFP-C-RS-VAMP3 shRNA containing sequence 3764 and pRFP-C-RS-scrambled shRNA were purchased from OriGene. Sequence 3764 is AAACGAGCGAGCCAAGTTGAAGAGGAAA.