Rat anti myelin basic protein mbp

Lipopolysaccharide (LPS; Escherichia coli 0111:B4) was obtained from InvivoGen. Creatine monohydrate (C3630), 3-guanidinopropionoic acid (GPA; G6878), and carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP; 370–86-5) were obtained from Sigma-Aldrich. MitoTracker Red FM (M22425), tetramethylrhodamine, ethyl ester (TMRE; T669), and propidium iodide (PI) (P3566) were obtained from Thermo Fisher Scientific. Primary antibodies for immunohistochemistry (IHC) were as follows: rat anti-CD11b (1:100; AbD Serotec), rabbit anti-cleaved caspase-3 (1:100; Cell Signaling Technology), rabbit anti-Olig2 (1:300; Millipore), rat anti-myelin basic protein (MBP, 1:200; AbD Serotec), mouse anti-CC1 (1:300; Millipore), and mouse anti-Nkx2.2 (1:100; Developmental Studies Hybridoma Bank). Primary antibodies for immunocytochemistry (ICC) were as follows: rabbit anti-Olig2 (1:500; Millipore), rat anti-MBP (1:500; AbD Serotec), anti-GFAP (1:500; Sigma-Aldrich), mouse anti-CC1 (1:200; Millipore), and anti-PDGFRα (1:300; BD PharMingen). Alexa Fluor 488 or 594 secondary antibodies (Thermo Fisher Scientific) were used at a concentration of 1:500. To label nuclei, cell and tissues were labeled with 1 μg/ml Hoechst in PBS for 5 min at room temperature (RT) (33342; Thermo Fisher Scientific).

Primary antibodies used for rodent experiments were rabbit anti-Caspr (Abcam, UK), mouse CC1 monoclonal antibody (Abcam, UK), rabbit anti-GAPDH (Sigma, UK), mouse anti-myelin-associated glycoprotein (MAG, Millipore, UK), rabbit anti-Kv1.1 (Abcam, UK), rat anti-myelin basic protein (MBP, Serotec, UK), chicken anti-neurofilament heavy chain (NFH, Encor Biotechnologies, Florida, United States), rabbit anti-Olig2 (Sigma, UK), mouse anti-O4 (Immunosolv, UK), mouse anti-NG2 (Millipore, UK), mouse anti-proteolipid protein (PLP, Millipore, UK), goat anti-Scribble (C20, Santa Cruz Biotechnology, US), mouse anti-pan sodium channel (Sigma, UK). Secondary antibodies used were Alexa 488-conjugated goat anti-rabbit IgG, Alexa 488-conjugated donkey anti-goat IgG, Alexa 568-conjugated donkey anti-rat IgG, Alexa 568-conjugated donkey anti-mouse IgG, and Alexa 568-conjugated goat anti-rabbit IgG (all from Invitrogen, UK), DyLight 405-conjugated donkey anti-rat IgG, and DyLight 405-conjugated donkey anti-chicken IgG (Stratech, UK).