Stock ssttm2.1 cre zjh j

All experiments involving living animals were approved by the National Council on Animal Care of the Italian Ministry of Health (authorization # 34/2015-PR and 125/2012-B) and carried out in accordance with the guidelines established by the European Communities Council Directive. The mouse strain Tg(Rbp4-cre)KL100Gsat/Mmcd (otherwise called Rbp4-cre), identification number 031125-UCD, was obtained from the Mutant Mouse Regional Resource Center, a NCRR-NIH funded strain repository, and was donated to the MMRRC by the NINDS-funded GENSAT BAC transgenic project. B6;129S6-Gt(ROSA)26Sor^{tm14(CAG-TdTomato)Hze}/J, id #007908 (otherwise called TdTomato line), B6;129P2-Pvalb^tm1(cre)Arbr/J, id #008069 (PV-cre line), STOCK Sst^{tm2.1(cre)Zjh}/J, id #013044 (SST-cre line) and B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J line 18, id #007612 (Thy1-ChR2 line) were purchased from the Jackson Laboratory (Bar Harbor, USA). All data were collected from mice of either sex. From postnatal days 30, animals were separated from the original cage and housed in group of up to four littermates per cage with ad libitum access to food and water in a 12:12 light-dark cycle. Age of animals used for each experimental dataset is specified in Method Details. The number of animals used for each experimental dataset is specified in the text or in the corresponding Figure legend.

The Swiss Federal Veterinary Office and the Canton of Vaud Veterinary Office (license number VD1628) approved all experimental procedures for animal usage. All experiments were performed according to the guidelines of the Swiss Federal Act on Animal Protection and Swiss Animal Protection Ordinance. We used PV-Cre mice (The Jackson Laboratory, #008069, B6;129P2-Pvalb^tm1(cre)Arbr/J) (Hippenmeyer et al. 2005 ) and SST-Cre mice (The Jackson Laboratory, #013044, STOCK Sst^{tm2.1(cre)Zjh}/J) (Taniguchi et al. 2011 ) crossed with ROSA-LSL-tdTomato mice (Ai14, The Jackson Laboratory, #007914, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J) (Madisen et al. 2010 (link)) to label PV⁺ and SST⁺ neurons with tdTomato (PV::tdTomato and SST::tdTomato). To label a subset of SST⁺ neurons, we used GIN mice (The Jackson Laboratory, #003718, FVB-Tg(GadGFP)45704Swn/J) (Oliva Jr. et al. 2000 (link)).

All experiments were approved by the Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences Research Committee, and all experimental procedures involving animals were carried out in strict accordance with the Research Committee’s animal use guidelines. Surgery was performed under full anesthesia, and every effort was made to minimize animal suffering. Male and female mice (6–14 weeks) were used in this study. We used the following mouse lines: PV-Cre (B6;129P2-Pvalbtm1(cre)Arbr/J, Jackson Laboratory, stock No.008069), D1-Cre (B6.Cg-Tg(Drd1a-cre)262Gsat/Mmcd, MMRRC, 030989-UCD), D2-Cre (B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmcd, MMRRC,032108-UCD), and SOM-Cre (STOCK Ssttm 2.1(cre) Zjh/J, Jackson Laboratory, stock No.013044). C57BL/6J wild-type mice were also used.