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Horseradish peroxidase hrp conjugated igg secondary antibody

Manufactured by Santa Cruz Biotechnology
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Horseradish peroxidase (HRP)-conjugated IgG secondary antibody is a laboratory reagent used in immunoassays and other applications. The HRP enzyme is covalently linked to an immunoglobulin G (IgG) antibody, which can bind to a primary antibody and generate a detectable signal.

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Lab products found in correlation

Bca kit, by Thermo Fisher Scientific (3 mentions) Bca protein assay kit, by Beyotime (2 mentions) Anti sirt1 antibody, by Santa Cruz Biotechnology (1 mentions) Qiaprep spin kit, by Qiagen (1 mentions) Anti human mac 1 antibody, by Thermo Fisher Scientific (1 mentions) Anti mouse igg isotype control antibody, by Thermo Fisher Scientific (1 mentions) R phycoerythrin pe conjugated mouse anti human mac 1 clone icrf44 antibody, by BD (1 mentions) Pe conjugated mouse igg isotype control clone mopc 21 antibody, by BD (1 mentions) Dneasy tissue kit, by Qiagen (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Mouse anti cdkl3 antibody, by Merck Group (1 mentions) Rabbit anti lc3a b antibody, by Cell Signaling Technology (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Thymidine t9250, by Merck Group (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) P akt, by Abcam (1 mentions) P pi3k, by Abcam (1 mentions) Pentobarbital sodium, by Solarbio (1 mentions) Dab developer, by Solarbio (1 mentions)

Close spelling variants of this product

Goat anti-mouse IgG horseradish peroxidase (HRP)conjugated secondary antibody Horseradish peroxidase (HRP)–conjugated immunoglobulin G (IgG) secondary antibody Horseradish peroxidase (HRP) conjugated secondary antibody against rabbit IgG Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody Horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody Horseradish peroxidase (HRP)-conjugated secondary antibodies Horseradish peroxidase-conjugated IgG secondary antibody Horseradish peroxidase-conjugated secondary antibodies Horseradish peroxidase (HRP)-conjugated antibody Peroxidase-conjugated secondary antibodies

5 protocols using horseradish peroxidase hrp conjugated igg secondary antibody

1

Molecular Mechanisms of Macrophage Activation

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Pam3CSK4 was purchased from InvivoGen Co. (San Diego, CA). The anti-human Mac-1 antibody (cat no. 16-0113) and anti-mouse IgG isotype control antibody (cat no. 16-4714) were purchased from eBioscience (San Diego, CA). The R-phycoerythrin (PE)-conjugated mouse anti-human Mac-1 (clone ICRF44; BD) antibody (cat no. 555388) and PE-conjugated mouse IgG isotype control (clone MOPC-21) antibody (cat no. 555749) were obtained from BD (San Diego, CA). Calcein AM (an acetomethoxy (AM) derivative of calcein) and luciferase reporter constructs containing NF-κB (cat no. 219078) and CREB (cat no. 219076) consensus-binding sites were purchased from Agilent Technologies (Santa Clara, CA, USA). The anti-SIRT1 antibody and horseradish peroxidase (HRP)-conjugated IgG (secondary antibody) were obtained from Santa Cruz Biotechnology Inc. (Beverly, MA). ImProm-II Reverse Transcription Kits were supplied by the Promega Corporation (Madison, WI). DNeasy Tissue Kits and QIAprep Spin Kits were purchased from Qiagen (GmbH, Germany).
Lee S.J., Baek S.E., Jang M.A, & Kim C.D. (2019). SIRT1 inhibits monocyte adhesion to the vascular endothelium by suppressing Mac-1 expression on monocytes. Experimental & Molecular Medicine, 51(4), 47.
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2

Protein Expression Analysis in ESCC Cells

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Briefly, the total protein in ESCC cells was extracted, followed by protein quantification using BCA Protein Assay Kit (Beyotime, Shanghai, China). 10% SDS-PAGE was used to separate protein samples. After transferring onto PVDF membranes (Millipore, Burlington, MA, United States), followed by blocking with 5% skim milk for 1 h and then separately incubated with mouse anti-CDKL3 antibody (Sigma-Aldrich, St. Louis, MO, United States) or mouse anti-ATG5 antibody (Santacruz, Santa Cruz, CA, United States) or rabbit anti-LC3A/B antibody (Cell Signaling Technology, Danvers, MA, United States) at dilutions of 1:1000 or 1:500 or 1:1000 overnight. Mouse anti-GAPDH (Santacruz, Santa Cruz, CA, United States) antibody (1:2000) was used as an internal control at 4°C overnight. Lastly, the visualization of protein bands was realized using a horseradish-peroxidase (HRP)-conjugated IgG secondary antibody (Santacruz, Santa Cruz, CA, United States).
Zhou S., Zhang M., Zhou C., Wang W., Yang H, & Ye W. (2020). CDKL3 Targets ATG5 to Promote Carcinogenesis of Esophageal Squamous Cell Carcinoma. Frontiers in Oncology, 10, 1602.
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3

HMGB1 and Transcription Factor Regulation

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Recombinant human HMGB1 (1690-HMB-050) was purchased from R&D System Inc. (Minneapolis, MN, United States). OPN (ab8448) antibody was purchased from Abcam (Cambridge, MA, United States). Activator protein 1 (AP-1; sc-12632) and β-actin (sc-47778) antibodies were purchased from Santa Cruz Biotechnology Inc. (Beverly, MA, United States). CCAAT/enhancer-binding protein beta (C/EBPβ; 3082S) antibody was purchased from Cell Signaling Technology (Beverly, MA, United States). Horseradish peroxidase (HRP)-conjugated IgG secondary antibody was purchased from Santa Cruz Biotechnology Inc. Thymidine (T9250) was purchased from Sigma-Aldrich (St. Louis, MO, United States).
Jeon E.Y., Baek S.E., Kim J.O., Choi J.M., Jang E.J, & Kim C.D. (2021). A Pivotal Role for AP-1-Mediated Osteopontin Expression in the Increased Migration of Vascular Smooth Muscle Cells Stimulated With HMGB1. Frontiers in Physiology, 12, 775464.
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4

Biochemical Assay Protocol for Alzheimer's Disease

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The 3,3-diaminobenzidine (DAB) reagent kit was obtained from Hubei Zhong Xin Biological Technology Co., Ltd. (Wuhan, China). All primers were obtained from Sunny Biological Science and Technology Co., Ltd. (Shanghai, China). Donepezil was purchased from Eisai Pharmaceutical Co., Ltd. (Suzhou, China) (Number: 100223A). Primary antibodies against PI3K, p-PI3K, Akt, p-Akt, GSK-3α, IDE, and Aβ were purchased from Abcam (Cambridge, UK). Primary antibody against β-actin was obtained from Bioworld Technology, Inc. (St. Louis Park, MN, USA). Horseradish peroxidase (HRP)-conjugated IgG secondary antibody was purchased from Santa Cruz Biotechnology Inc (Delaware, USA), and a BCA protein assay kit was obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China).
Lin S.Y., Wang T.Q., Xu L.T., Lai X.X., Shen Y., Lin J.W., Gao S.Y, & Hu H.Y. (2020). Qingxin Kaiqiao Recipe Improves Cognitive Performance, Inhibits Apoptosis, and Reduces Pathological Deposits in APP/PS1 Double Transgenic Mice via the PI3K/Akt Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 3019674.
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5

Antibody-Based Protein Expression Analysis

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Antibodies were from the following companies: rabbit monoclonal anti-GAPDH (AP0063; Biogot Technology, Shanghai, People's
Republic of China); rabbit monoclonal anti-Cadherin5 (A02632-2; Boster Biological Technology, Wuhan, People's
Republic of China); rabbit monoclonal anti-SOD1, anti-MMP9, anti-HO1, anti-VEGF, anti-Caspase3 (CAPS3) (12,452-1, 19,003-1, 10,375-2, 10,269-1, 10,701-1; Proteintech Group, Chicago, IL, USA); rabbit monoclonal anti-endothelial anti-cytochrome c (CYC), eNOS and anti-Bax (14,796, 11940S and 32,027; Cell Signaling Technology, Beverly, MA, USA); horseradish peroxidase (HRP)-conjugated IgG secondary antibody (Santa Cruz Biotechnology, Dallas, TX, USA). Pentobarbital sodium, DAB developer, and H&E staining kit were acquired from Solarbio Science & Technology (Beijing, People's Republic of China). SOD, GSH, and MDA assay kits were from Jiancheng Technology (Nanjing, People's
Republic of China). The BCA Kit was purchased from Thermo Fisher Scientific (Rockford, IL, USA). The ECL Plus Reagent Kit was acquired from PerkinElmer Life Sciences (Waltham, MA, USA).
Lin J., Jia C., Wang Y., Jiang S., Jia Z., Chen N., Sheng S., Li S., Jiang L., Xu H., Zhou K, & Chen Y. (2019). Therapeutic potential of pravastatin for random skin flaps necrosis: involvement of promoting angiogenesis and inhibiting apoptosis and oxidative stress. Drug Design, Development and Therapy, 13, 1461-1472.
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