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Rodent diet 5001

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Sourced in United States, Sao Tome and Principe

Rodent Diet 5001 is a laboratory animal feed designed for rodents. It provides a complete and balanced nutrition to support the growth and health of rodents in a research setting. The diet is formulated to meet the nutritional requirements of rodents and is available in various forms, such as pellets or extruded nuggets.

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Lab products found in correlation

D12492, by Research Diets (4 mentions) Sprague dawley rats, by Taconic Biosciences (3 mentions) Formulab diet 5008, by LabDiet (2 mentions) Sprague dawley rats, by BioLASCO (1 mentions) Female c57bl 6 mice, by Jackson ImmunoResearch (1 mentions) C57bl 6 male mice, by Jackson ImmunoResearch (1 mentions) C57bl 6 mice, by Taconic Biosciences (1 mentions) Sprague dawley rats, by Inotiv (1 mentions) D12079bi, by Research Diets (1 mentions) Polycarbonate cages, by Ancare (1 mentions) Fructose, by Bio Basic (1 mentions)

Spelling variants of this product

Laboratory Rodent Diet 5001 (83 citations) Rodent 5001 (5 citations) 23 Rodent Diet 5001 (4 citations) Diet 5001 (3 citations) Rodent Lab Chow diet 5001 (3 citations) Lab Rodent Diet 5001 (2 citations)

43 protocols using rodent diet 5001

1

Aging Mice Housing and Feeding

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The experimental procedures were performed according to the National Institutes of Health guidelines and approved by the Institutional Animal Care and Use Committee at the Nathan Kline Institute. Mice were housed in standard mouse cages, with a 12 h light/dark cycle. Mice had food (Rodent diet 5001; LabDiet) and water ad libitum. During gestation and until weaning, mice were fed chow formulated for breeding (Formulab diet 5008; LabDiet). Mice were weaned at 23–25 d of age and then were fed a standard rodent chow after weaning (Rodent diet 5001, LabDiet). After weaning mice were housed with littermates of the same sex (maximum four mice per cage). For the experimental procedures, two ages were selected: 2–3 months (mean 98.5 ± 5.4 d; range 70–101, n = 12) and 11–20 months (mean 451.8 ± 23 d; range 337–611, n = 20). Ages in the Results that are in months were calculated by dividing the age in days by 30.3 because the average number of days/month is 30.3.
Criscuolo C., Chartampila E., Ginsberg S.D, & Scharfman H.E. (2024). Dentate Gyrus Granule Cells Show Stability of BDNF Protein Expression in Mossy Fiber Axons with Age, and Resistance to Alzheimer’s Disease Neuropathology in a Mouse Model. eNeuro, 11(3), ENEURO.0192-23.2023.
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2

Investigating Murine Aging Across Lifespan

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The experimental procedures were performed according to National Institutes of Health guidelines and approved by the Institutional Animal Care and Use Committee at the Nathan Kline Institute. Mice were housed in standard mouse cages, with a 12 hr light-dark cycle. Mice had food (Rodent diet 5001; LabDiet) and water ad libitum. During gestation and until weaning, mice were fed chow formulated for breeding (Formulab diet 5008; LabDiet). Mice were weaned at 23–25 days of age and then were fed a standard rodent chow after weaning (Rodent diet 5001, LabDiet). After weaning mice were housed with littermates of the same sex (maximum four mice per cage). For the experimental procedures, two ages were selected: 2–3 months (mean 98.5 ± 5.4 days; range 70–101, n = 12) and 11–20 months (mean 451.8 ± 23 days; range 337–611, n = 20). Ages in the Results that are in months were calculated by dividing the age in days by 30.3 because the average number of days/month is 30.3.
Criscuolo C., Chartampila E., Ginsberg S.D, & Scharfman H.E. (2023). Stability of dentate gyrus granule cell mossy fiber BDNF protein expression with age and resistance of granule cells to Alzheimer’s disease neuropathology in a mouse model. bioRxiv.
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3

Chronic Food Restriction in Rats

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All subjects were male Sprague–Dawley rats (Taconic Farms, Germantown, NY, USA) weighing 350–400 g at the time of arrival in the central animal facility where they were housed in individual plastic cages with free access to standard pelleted lab chow (Rodent Diet #5001, Lab Diet, St. Louis, MO) and water unless otherwise noted. The animal room was maintained on a 12-h light/dark cycle, with lights on at 0600 h. Half the subjects in each experiment were placed on a chronic food restriction regimen whereby daily food allotment was initially limited to 10 g of chow, delivered at 1700 h, until a 20% decrease in body weight was attained. Attainment of the target body weight typically took 15-20 days. Daily feeding was then titrated to clamp body weight at that value for 1-2 additional weeks until brain harvesting in the biochemistry and electrophysiology experiments, or 1 week until initiation, and an additional 3 weeks through completion, of the behavioral experiment.
All experimental procedures were approved by the New York University School of Medicine Institutional Animal Care and Use Committee and were performed in accordance with the “Principles of Laboratory Animal Care” (NIH publication number 85-23). All efforts were made to minimize animal suffering, to reduce the number of animals used and to utilize alternatives to in vivo techniques.
Ouyang J., Carcea I., Schiavo J.K., Jones K.T., Rabinowitsch A., Kolaric R., Cabeza de Vaca S., Froemke R.C, & Carr K.D. (2017). Food Restriction Induces Synaptic Incorporation of Calcium-Permeable AMPA Receptors in Nucleus Accumbens. The European journal of neuroscience, 45(6), 826-836.
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4

Sprague–Dawley Rat Housing and Feeding

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Sprague–Dawley rats were obtained from BioLASCO Co., Ltd, Taipei City, Taiwan. They were housed in animal facility at University of Taipei. All animals were maintained in a temperature-controlled room (21 ± 2° C) under a 12-h light–dark cycle (6:00 a.m.–6:00 p.m.) and a relative humidity of 45-55%. Every 2 animals were housed for each cage. They were fed with standard rat chow (Rodent Diet 5001, LabDiet, St. Louis, MO, USA) and normal tap water in feeding bottle, recorded the amount of food intake and body weight at the same time twice a day. Food and water intake were recorded.
Lin Y.H., Tsai S.C., Chuang S.J., Harris M.B., Masodsai K., Chen P.N., Hsieh C.C., Killian T., Huang C.Y, & Kuo C.H. (2021). Whole-life body composition trajectory and longevity: role of insulin. Aging (Albany NY), 13(7), 9719-9731.
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5

Anxiety-like Behavior in Male and Female Mice

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Male C57Bl/6 mice (n = 60), 7 weeks of age, were acquired from The Jackson Laboratory (Bar Harbor, ME). Each animal was singly housed in a colony room with a 12/12 h light-dark cycle (lights off at 7:00 p.m.) at an ambient temperature of 22–23 °C. Additional feMale C57Bl/6 mice, 4 weeks of age (n = 16), were acquired from the Jackson Laboratory for the Female Urine Sniffing Test (FUST). Adult male Long Evans rats (n = 30; 400+ grams upon arrival) purchased from Harlan (Indianapolis, IN, USA) served as stimulus (predator) animals. Rats were pair-housed and had free access to standard lab chow (Rodent Diet 5001; Lab Diet, Brentwood, MO, USA) and water. Rats were housed in a separate room from mice and maintained on a 12/12 h reverse light/dark cycle (lights off at 07:00 a.m.) at an ambient temperature of 22–23 °C. Upon arrival at the animal facility, mice and rats were allowed seven days of acclimatization prior to experimentation during which they were all fed standard rodent diet (Lab Diet 5001). All studies and animal protocols were approved and guided by the Institutional Animal Care and Use Committee at Emory University (#2002041).
Elizabeth de Sousa Rodrigues M., Bekhbat M., Houser M.C., Chang J., Walker D.I., Jones D.P., Oller do Nascimento C.M., Barnum C.J, & Tansey M.G. (2016). Chronic psychological stress and high-fat high-fructose diet disrupt metabolic and inflammatory gene networks in the brain, liver, and gut and promote behavioral deficits in mice. Brain, behavior, and immunity, 59, 158-172.
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6

Rat Housing and Feeding Protocol

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The rats were adult male Sprague–Dawley rats born in our lab or purchased from Taconic Farms (Germantown, NY) or Charles River (Wilmington, MA) weighing 345–423 g (10–12 weeks old) at the start of the experiments. They were maintained in a central animal facility with a 12-h light/ dark period (lights on at 6 am). The rats were singly housed in plastic cages with bedding and free access to standard lab pellets (Rodent Diet #5001, Lab Diet, St. Louis, MO) and water, except at least one hour before and during the time spent in IG infusion chambers, behavioral conditioning and testing sessions or after assigned a diet regime.
Woods C.A., Guttman Z.R., Huang D., Kolaric R.A., Rabinowitsch A.I., Jones K.T., Cabeza de Vaca S., Sclafani A, & Carr K.D. (2016). Insulin receptor activation in the nucleus accumbens reflects nutritive value of a recently ingested meal. Physiology & behavior, 159, 52-63.
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7

Generation and Characterization of Angptl8 Knockout Mice

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Angptl8−/− mice (75% C57BL/6NTac and 25% 129/SvEvTac background) were generated by homologous recombination using Regeneron's VelociGene® technology as previously described (Wang et al., 2013 (link)). Mice were housed (male; 1-5 per cage) in a controlled environment (12-h light/dark cycle, 22±1 °C, 60–70% humidity) and fed ad libitum with standard chow (Purina Laboratory Rodent Diet 5001, LabDiet). Some mice were fed a high-fat diet (Research Diets, D12492; 60% fat by calories). For all studies reported in this paper, comparisons were made between wild-type and knock-out littermates. C57Bl/6 mice (males, 24-28 g; Taconic) were used in the hydrodynamic tail vein overexpression study. All animal procedures were conducted in compliance with protocols approved by the Regeneron Pharmaceuticals Institutional Animal Care and Use Committee.
Gusarova V., Alexa C.A., Na E., Stevis P.E., Xin Y., Bonner-Weir S., Cohen J.C., Hobbs H.H., Murphy A.J., Yancopoulos G.D, & Gromada J. (2014). ANGPTL8 (Betatrophin) Does Not Control Pancreatic Beta Cell Expansion. Cell, 159(3), 691-696.
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8

Estrous Stage Effects on MCH Eating in Males

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Based on studies that have shown that the endogenous estrous stage is a critical determinant of MCH effects on eating in females17 (link),33 , we have chosen to use male rats for this manuscript. For all experiments, male Sprague-Dawley rats (Envigo, Indianapolis, IN) weighing 300–400 g were used and individually housed in shoebox cages. Except where noted, rats were given ad libitum access to chow (Rodent Diet 5001, LabDiet, St. Louis, MO) and water. Rats were housed in a 12 h:12 h reverse light/dark cycle (lights off at 10:00 a.m.) or a 12 h:12 h light/dark cycle (lights off at 6:00 p.m.). All experiments were performed in accordance with NIH Guidelines for the Care and Use of Laboratory Animals, and all procedures were approved by the Institutional Animal Care and Use Committee of the University of Southern California.
Subramanian K.S., Lauer L.T., Hayes A.M., Décarie-Spain L., McBurnett K., Nourbash A.C., Donohue K.N., Kao A.E., Bashaw A.G., Burdakov D., Noble E.E., Schier L.A, & Kanoski S.E. (2023). Hypothalamic melanin-concentrating hormone neurons integrate food-motivated appetitive and consummatory processes in rats. Nature Communications, 14, 1755.
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9

Dietary Regulation in Rat Experiments

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Experimental procedures were approved by the Institutional Animal Care and Use Committee at the New York University School of Medicine and were consistent with the Principles of Laboratory Care (NIH Publication no. 85-23). All subjects were mature male Sprague-Dawley rats (Taconic Farms, Germantown, NY) weighing 350-400 g (10-12 weeks old) at the start of each experiment. Rats were housed on a 12-hour light:dark photoperiod with lights on between 6am and 6pm in a central animal facility. All subjects were singly housed in individual plastic cages with bedding and free access to water.
Ad libitum-fed animals had free access to standard lab pellets (Rodent Diet #5001, Lab Diet, St. Louis, MO). Food restricted animals followed a feeding regimen described previously (e.g.,Cabeza de Vaca and Carr 1998 (link)), in which rats received 40% of ad libitum intake of chow (10 g) daily, delivered to the home cage at 5 pm, until body weight was reduced by 20% (~2 weeks). Daily feeding was then titrated to maintain the new body weight for one week before resuming behavioral testing. Restricted feeding and body weight were maintained until the end of experimentation.
Zheng D., Cabeza de Vaca S., Jurkowski Z, & Carr K.D. (2015). Nucleus Accumbens AMPA Receptor Involvement in Cocaine Conditioned Place Preference under Different Dietary Conditions in Rats. Psychopharmacology, 232(13), 2313-2322.
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10

Effects of Diet on Glucose and Saccharin Lickometry in Rats

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Rats in Experiment 1 (effect of NAc InsAb on glucose lickometry), Experiment 2 (persistence of NAc InsAb effect) and Experiment 3 (effect of NAc InsAb on saccharin lickometry) had free access to standard lab pellets (Rodent Diet #5001, Lab Diet, St. Louis, MO) in the home cage. Rats in Experiment 4 (effect of ‘Western’ diet on glucose lickometry) were maintained for twelve weeks on either a ‘Western’ (D12079Bi, Research Diets Inc., New Brunswick NJ) or control (D14042701i) diet. The ‘Western’ diet is 35% sucrose and 20% butter fat by weight. The control diet is matched for protein, vitamin, and mineral content but contains 58% cornstarch and 3.5% milk fat by weight.
Carr K.D, & Weiner S.P. (2022). Effects of Nucleus Accumbens Insulin Inactivation on Microstructure of Licking for Glucose and Saccharin in Male and Female Rats. Physiology & behavior, 249, 113769.
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